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Soil analysis makes for developing precision agriculture and monitoring land quality, while the models available for spectroscopy-based chemometrics are constrained by limited samples from small areas. The paper proposed sample expansion and model construction based on spectral difference and content difference, realizing data augmentation and deep learning applied to original samples with limited numbers. The spectral subtraction based on maximum or minimum values exploited the maximum or minimum values to acquire the spectral difference and content difference, which provided a new data form for model construction. Keeping enhanced samples whose spectral difference and content difference were all zero was useful for improving model performance. Augmentation of all data or training data based on maximum or minimum values-based spectral subtraction, which sorted the contents and made them the maximum or minimum values in sequence, achieved sample expansion by the spectral difference and content difference. The model utilized the random vector functional link (RVFL) network, extreme learning machine (ELM), and one-dimensional convolutional neural network (1D CNN), which could predict the content of new samples through ensemble averaging when predicting content difference. The experimental result showed the model of the spectral subtraction based on maximum or minimum values had a similar performance to that of the original samples. Augmentation of all data improved model performance by only RVFL and ELM. Augmentation of training data verified 1D CNN was better than RVFL and ELM. The paper implements a new data augmentation method and applies CNN to original samples with inadequate numbers, which lays the foundation for an improved model and applying spectral preprocessing., Competing Interests: Declaration of competing interest Lushan Wan, Dong Xiao, Zhizhong Mao, Zhenni Li has patent #202311576119.9 pending to Northeastern University. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)