1. Comparative pharmacokinetic study of paeoniflorin and albiflorin after oral administration of Radix Paeoniae Rubra in normal rats and the acute cholestasis hepatitis rats.
- Author
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Jiang F, Zhao Y, Wang J, Wei S, Wei Z, Li R, Zhu Y, Sun Z, and Xiao X
- Subjects
- Acute Disease, Administration, Oral, Animals, Anti-Inflammatory Agents, Non-Steroidal administration & dosage, Anti-Inflammatory Agents, Non-Steroidal chemistry, Benzoates administration & dosage, Benzoates chemistry, Bridged-Ring Compounds administration & dosage, Bridged-Ring Compounds chemistry, Cholestasis drug therapy, Drugs, Chinese Herbal administration & dosage, Drugs, Chinese Herbal chemistry, Glucosides administration & dosage, Glucosides chemistry, Hepatitis drug therapy, Humans, Male, Monoterpenes, Random Allocation, Rats, Rats, Sprague-Dawley, Sensitivity and Specificity, Time Factors, Anti-Inflammatory Agents, Non-Steroidal pharmacokinetics, Benzoates pharmacokinetics, Bridged-Ring Compounds pharmacokinetics, Drugs, Chinese Herbal pharmacokinetics, Glucosides pharmacokinetics, Paeonia chemistry
- Abstract
A comparative study was designed and conducted to compare the pharmacokinetic difference of paeoniflorin and albiflorin after oral administration of Radix Paeoniae Rubra to normal rats and the acute cholestasis hepatitis rats induced by alpha-naphthylisothiocyanate (ANIT). UPLC-ESI-MS/MS method was employed to determine the level of paeoniflorin and albiflorin in rat plasma using geniposide as the internal standard (IS). Unpaired Student's t-test was used for the statistical comparison. The investigation showed that there were significant differences between the normal rats and the acute cholestasis hepatitis rat groups in calculated parameters, such as AUC(0-t), AUC(0-∞), T(max) and CLz/F. The results indicated that acute liver injury in rats could alter the pharmacokinetics of drug. Since patients are the final users of the drug, it is essential to investigate the pharmacokinetics of the drug in disease status. Therefore, we used normal rats and the acute cholestasis hepatitis rats to study pharmacokinetics of Radix Paeoniae Rubra with the purpose of examining the influence of disease on the metabolic course., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2012
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