30 results on '"Wu, Y. C."'
Search Results
2. List of Contributors
- Author
-
Al-Hussinee, L., primary, Batts, W.N., additional, Becker, J., additional, Bistolas, K., additional, Button, J.B., additional, Chi, S.-C., additional, Clark, K.F., additional, Cowley, J.A., additional, Dale, O.B., additional, de Oliveira Viadanna, P.H., additional, Doszpoly, A., additional, Evensen, Ø., additional, Gjessing, M.C., additional, Godoy, M.G., additional, Hanson, L., additional, Haugland, Ø., additional, Hewson, I., additional, Hick, P., additional, Hong, J.-R., additional, Jackson, E.W., additional, Kibenge, F.S.B., additional, Kibenge, M.J.T., additional, LaPatra, S., additional, Lumsden, J.S., additional, Meyers, T.R., additional, Mikalsen, A.B., additional, Misk, E., additional, Mizutani, T., additional, Mor, S.K., additional, Munang’andu, H.M., additional, Mutoloki, S., additional, Nagy, É., additional, Naim, S., additional, Phelps, N.B.D., additional, Quackenbush, S., additional, Renault, T., additional, Schütze, H., additional, Sindre, H., additional, Sunarto, A., additional, Taksdal, T., additional, Tuboly, T., additional, van Beurden, S.J., additional, Waltzek, T., additional, Wang, C.S., additional, Weli, S.C., additional, Whittington, R., additional, and Wu, Y.-C., additional
- Published
- 2016
- Full Text
- View/download PDF
3. Fabrication, microstructure and mechanical properties of the as-rolled ZW31/PMMCs laminate
- Author
-
Zhang, X.-C., Wang, C.-J., Deng, K.-K., Nie, K.-B., Gan, W.-M., Liang, W., and Wu, Y.-C.
- Abstract
In present work, the ductile Mg–Zn–Y alloy (ZW31) was introduced into the 10 μm 15 vol% SiCp/AZ91 composites (PMMCs) to coordinate the deformation of PMMCs during the rolling process, then the ZW31/PMMCs laminate with the thickness of ~1 mm was prepared successfully. The microstructure and mechanical properties of the as-rolled ZW31/PMMCs laminate before and after the annealing process was investigated, the results indicated that existence of ZW31 alloy layer can absorb energy and relieve stress concentration of PMMCs layer by the formation of twins, which not only break through the bottlenecks of poor toughness of PMMCs, but also make it possible for the rolling of PMMCs sheet. The recrystallization behavior of the ZW31 alloy layer and PMMCs layer was completely different during the subsequent annealing process. For the ZW31 alloy layer, recrystallization induced by twinning, while the recrystallization in PMMCs layer related to the introduced particle. The typical rolling texture was formed in the ZW31/PMMCs laminate, and the intensity of which was mainly controlled by the ZW31 alloy. Application of annealing process improved the elongation of the ZW31/PMMCs laminate, however, at the expense of strength. Cracks originate in different positions of PMMCS layer and then connect with each other along the direction which perpendicular to the stress. The existence of ZW31 alloy layer inhibits the propagation of crack and is propitious to improve the ductility of ZW31/PMMCs laminate.
- Published
- 2019
4. The effects of feeding time and time-restricted feeding on the fattening traits of White Roman geese.
- Author
-
Ho SY, Wu YC, Chen YH, and Yang SK
- Subjects
- Animal Feed analysis, Animals, Diet, Female, Male, Random Allocation, Geese physiology
- Abstract
This study comprises two trials that investigated the effects of feeding time and time-restricted feeding on the fattening traits and plasma metabolite levels of White Roman geese. In Trial I, 24 geese aged 8 weeks of each sex were allowed free access to a fattening diet for 1 h either in the morning (morning-feeding group) or afternoon (afternoon-feeding group). At 12 weeks of age, blood samples were collected hourly for 4 h, beginning 1 h after feeding to determine the plasma levels of glucose, triacylglycerols and uric acid. The results showed a lower (P<0.05) daily feed intake (DFI) and daily gain (DG) and higher (P<0.05) feed efficiency (FE) for the morning-feeding group compared with those of the afternoon-feeding group. In addition, the postprandial plasma levels of glucose, triacylglycerols and uric acid did not differ (P>0.05) between groups. In Trial II, 12 geese aged 8 weeks of each sex were randomly assigned to either the ad libitum feeding group (control group) or time-restricted feeding group (restricted group). The geese in the control group were fed a fattening diet ad libitum, whereas those in the restricted group were allowed access to the diet for 2 h every morning. All geese were killed at 13 weeks of age and their carcass traits were evaluated. The results showed a lower DFI and DG and higher FE for the restricted group compared with those of the control group (P<0.05). In addition, the restricted group exhibited lower visceral and abdominal fat and higher empty digestive tract and liver weights than those of the control group (P<0.05). The results showed that time-restricted feeding in the morning resulted in superior DG and FE compared with feeding in the afternoon. Moreover, time-restricted feeding implemented in the morning during the fattening period reduced DFI and increased FE in geese compared with ad libitum feeding.
- Published
- 2014
- Full Text
- View/download PDF
5. Dental diagnostic X-ray exposure and risk of benign and malignant brain tumors.
- Author
-
Lin MC, Lee CF, Lin CL, Wu YC, Wang HE, Chen CL, Sung FC, and Kao CH
- Subjects
- Adult, Female, Humans, Male, Middle Aged, Radiography, Dental trends, Risk Factors, Taiwan epidemiology, Young Adult, Brain Neoplasms diagnosis, Brain Neoplasms epidemiology, Neoplasms, Radiation-Induced diagnosis, Neoplasms, Radiation-Induced epidemiology, Population Surveillance methods, Radiography, Dental adverse effects
- Abstract
Background: This study evaluates the risk of benign brain tumors (BBTs) and malignant brain tumors (MBTs) associated with dental diagnostic X-ray, using a large population-based case-control study., Materials and Methods: We identified 4123 BBT cases and 16 492 controls without BBT (study 1) and 197 MBT cases and 788 controls without MBT (study 2) from Taiwan National Health Insurance claim data. The risks of both types of tumor were estimated in association with the frequency of received dental diagnostic X-ray., Results: The mean ages were ~44.2 years in study 1 and 40.6 years in study 2. Multivariable unconditional logistic regression analysis showed that the risk of BBT increases as the frequency of received dental diagnostic X-ray increases. The BBT odds ratio increased from 1.33 [95% confidence interval (CI) 1.22-1.44] for those with annual mean X-ray examination of less than one to 1.65 (95% CI 1.37-1.98) for those with three or more X-ray examinations, after controlling for comorbidities. No significant association was found between MBTs and dental diagnostic X-ray exposure., Conclusions: Exposure to dental diagnostic X-rays in oral and maxillofacial care increases the risk of BBTs, but not MBTs.
- Published
- 2013
- Full Text
- View/download PDF
6. Mechanism of protection of bystander cells by exogenous carbon monoxide: impaired response to damage signal of radiation-induced bystander effect.
- Author
-
Han W, Yu KN, Wu LJ, Wu YC, and Wang HZ
- Subjects
- Animals, CHO Cells, Carbon Monoxide pharmacology, Coculture Techniques, Cricetinae, Cricetulus, Female, Organometallic Compounds metabolism, Signal Transduction, Bystander Effect drug effects, Bystander Effect radiation effects, Nitric Oxide metabolism
- Abstract
A protective effect of exogenous carbon monoxide (CO), generated by CO releasing molecule ticarbonyldichlororuthenium (II) dimer (CORM-2), on the bystander cells from the toxicity of radiation-induced bystander effect (RIBE) was revealed in our previous study. In the present work, a possible mechanism of this CO effect was investigated. The results from medium transfer experiments showed that α-particle irradiated Chinese hamster ovary (CHO) cells would release nitric oxide (NO), which was detected with specific NO fluorescence probe, to induce p53 binding protein 1 (BP1) formation in the cell population receiving the medium, and the release peak was found to be at 1h post irradiation. Treating the irradiated or bystander cells separately with CO (CORM-2) demonstrated that CO was effective in the bystander cells but not the irradiated cells. Measurements of NO production and release with a specific NO fluorescence probe also showed that CO treatment did not affect the production and release of NO by irradiated cells. Protection of CO on cells to peroxynitrite, an oxidizing free radical from NO, suggested that CO might protect bystander cells via impaired response of bystander cells to NO, a RIBE signal in our research system., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
7. Salvage surgery after failed chemoradiotherapy in squamous cell carcinoma of the esophagus.
- Author
-
Chao YK, Chan SC, Chang HK, Liu YH, Wu YC, Hsieh MJ, Tseng CK, and Liu HP
- Subjects
- Carcinoma, Squamous Cell drug therapy, Carcinoma, Squamous Cell mortality, Carcinoma, Squamous Cell radiotherapy, Chi-Square Distribution, Combined Modality Therapy, Esophageal Neoplasms drug therapy, Esophageal Neoplasms mortality, Esophageal Neoplasms radiotherapy, Female, Hospital Mortality, Humans, Male, Middle Aged, Neoadjuvant Therapy, Neoplasm Recurrence, Local, Proportional Hazards Models, Registries, Retrospective Studies, Risk Factors, Survival Rate, Treatment Failure, Treatment Outcome, Carcinoma, Squamous Cell surgery, Esophageal Neoplasms surgery, Esophagectomy methods, Salvage Therapy
- Abstract
Aims: To investigate the survival benefit and preoperative risk factors for hospital mortality of salvage surgery in esophageal cancer patients who had locoregional residual/recurrent tumor after definitive chemoradiotherapy., Methods: We retrospectively reviewed the esophageal cancer patients who presented at our hospital from 1997 to 2004. Forty-seven patients who had squamous cell cancer and developed locoregional recurrent/persistent disease after primary definitive chemoradiotherapy were elected. Twenty-seven of them received salvage esophagectomy (group 1) and the other 20 underwent non-operative treatment only (group 2). In order to assess the surgery-related mobility and mortality in group 1, 191 patients who received neoadjuvant chemoradiotherapy followed by operation during the same time period were also enrolled (group 3)., Results: The 5-year overall survival of group 1 patients was 25.4%. In contrast, all of the patients in the group 2 died within 16.7 months. The difference was statistically significant (p=0.0029). In comparison with group 3, group 1 patients had significantly more surgery-related complications and hospital mortality. In univariate analysis for preoperative risk factors, a low albumin or hemoglobulin level was associated with high hospital mortality in group 1 (p=0.004 and 0.003, respectively). After multivariate analysis, only the low albumin level remained borderline significance. As for disease specific survival after salvage surgery, R0 resection was the only independent prognosticator (p=0.049)., Conclusion: Salvage surgery provides survival benefit in esophageal cancer patients with locoregional persistent or recurrent disease after primary definitive chemoradiotherapy. Preoperative albumin and hemoglobulin levels are associated with hospital mortality and may aid in selecting suitable patient for salvage surgery.
- Published
- 2009
- Full Text
- View/download PDF
8. The management of papillary lesion of the breast by core needle biopsy.
- Author
-
Tseng HS, Chen YL, Chen ST, Wu YC, Kuo SJ, Chen LS, Wu HK, and Chen DR
- Subjects
- Adult, Aged, Breast pathology, Breast surgery, Breast Neoplasms diagnostic imaging, Breast Neoplasms surgery, Carcinoma, Papillary diagnostic imaging, Carcinoma, Papillary surgery, Female, Humans, Middle Aged, Retrospective Studies, Ultrasonography, Interventional, Ultrasonography, Mammary, Biopsy, Needle methods, Breast Neoplasms pathology, Carcinoma, Papillary pathology
- Abstract
Background: The purpose of this study was to determine the accuracy of core needle biopsy (CNB) diagnoses of papillary breast lesions, and to identify the risk factors and histologic features, compared with excisional biopsy (EB)., Method: We retrospectively reviewed 1,682 sonographically guided core needle biopsies performed at one single medical center from January 2001 through December 2005, and identified 41 papillary lesions. Surgical correlation was available for 35 cases, 2 cases were loss follow-up and 4 cases diagnosed as papilloma by core needle biopsy were followed up with imaging for at least 24 months., Results: The pathologic diagnoses for the 35 papillary lesions obtained at core biopsy were benign in 24 cases, atypical in 7, and malignant in 4. Of those diagnosed as benign lesions at CNB, 7/24 turned out to be malignant. In the hyperplasia group, 5/7 were malignant. The total upgrade rate was 39%. The positive predict value was 100% and negative predict value was 61%., Conclusion: All papillary lesions of the breast diagnosed by CNB should be excised because a substantial number of lesions were upgraded of diagnoses at excision.
- Published
- 2009
- Full Text
- View/download PDF
9. Personal experience of ultrasound-guided 14-gauge core biopsy of breast tumor.
- Author
-
Wu YC, Chen DR, and Kuo SJ
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Breast pathology, Breast Neoplasms diagnostic imaging, Breast Neoplasms surgery, False Negative Reactions, Female, Humans, Middle Aged, Sensitivity and Specificity, Biopsy, Fine-Needle, Breast Neoplasms pathology, Ultrasonography, Interventional
- Abstract
Aim: This study was aimed to examine the efficacy of ultrasound-guided core needle biopsy of breast tumor and compared with the results of previous publications., Methods: From January 2001 to September 2003, 546 lesions in 513 consecutive patients with the identification of a tumor on ultrasound examination categorized belong and above C3 according to BIRADS (Breast Image Reporting and Data Systems). A minimum of three samples was taken during the biopsy process., Results: The patients' ages ranged from 17 to 89 years (mean, 43 years); tumors were from 5.7 to 41.6 mm in diameter (mean, 20.3 mm). There were 341 lesions with benign findings, 202 lesions with malignancy and 3 lesions with atypical ductal hyperplasia (ADH). Underestimates were found in five patients. Two patients were "ADH-DCIS (ductal carcinoma in situ) underestimate", and three patients were "DCIS-IDC (invasive ductal carcinoma) underestimate". The ADH underestimation rate was 40% (2/5) and DCIS underestimation rate was 43% (3/7). No false-positive results were observed. According to this study, the accuracy rate was 99%, sensitivity rate 97.6%, specificity rate 100%, and false-negative rate 2.4%., Conclusions: Both palpable and impalpable breast lesions should be examined under image guidance and automated core biopsy is the technique of first choice. Fourteen-gauge core biopsy can provide a definitive diagnosis in 99% of solid tumors in this series.
- Published
- 2006
- Full Text
- View/download PDF
10. Cyclooxygenase-2 in human non-small cell lung cancer.
- Author
-
Fang HY, Lin TS, Lin JP, Wu YC, Chow KC, and Wang LS
- Subjects
- Aged, Butyrates pharmacology, Cyclooxygenase 2, Female, Humans, Immunoblotting, Immunohistochemistry, Inflammation Mediators pharmacology, Male, Membrane Proteins, Platelet Activating Factor pharmacology, Reverse Transcriptase Polymerase Chain Reaction, Tetradecanoylphorbol Acetate pharmacology, Tumor Cells, Cultured, Carcinoma, Non-Small-Cell Lung enzymology, Isoenzymes metabolism, Lung Neoplasms enzymology, Prostaglandin-Endoperoxide Synthases metabolism
- Abstract
Aim: Recent studies report that the expression of cyclooxygenase (COX) in non-small cell lung cancer (NSCLC) is increased, especially in adenocarcinoma. Platelet activating factor (PAF), n-sodium butyrate (n-BT), and phorbol myristate acetate (PMA) are important mediators of the inflammatory process., Method: Expression of COX-2 in 67 stage 1 NSCLC paraffin-embedded tumor samples was determined by immunohistochemistry (IHC). Four NSCL cell lines were incubated and stimulated by PAF, n-BT and PMA for 48 h. Expression of COX-2 was determined by IHC, immunoblotting, and reverse transcription-polymerase chain reaction (RT-PCR)., Result: IHC showed increasing immunoreactivity in 35 of 67 (52%) in stage I NSCLC, 31 of 53 (59%) in adenocarcinoma and 13 of 15 (87%) in bronchoalveolar cell carcinoma, but only 2 of 12 (17%) in epidermoid carcinoma. The COX-2 expression in NSCLC cells was 75% (3/4) and the COX-1 expression in NSCLC cells was 100% (4/4). After stimulation with PMA, n-BT, PAF and n-BT + PAF, the COX-2 expression in NSCLC cells was significantly increased in all cell lines., Conclusions: The expression of COX-2 in NSCLC cells is high and was up-regulated by PMA, n-BT and PAF. We consider that COX-2 inhibitors will play an important role in the therapy of NSCLC., (Copyright 2002 Elsevier Science Ltd.)
- Published
- 2003
- Full Text
- View/download PDF
11. Minimal access thoracic surgery for esophageal hemangioma.
- Author
-
Wu YC, Liu HP, Liu YH, Hsieh MJ, and Lin PJ
- Subjects
- Aged, Female, Humans, Minimally Invasive Surgical Procedures methods, Thoracic Surgical Procedures methods, Esophageal Neoplasms surgery, Hemangioma surgery
- Abstract
Esophageal hemangioma is a rare tumor. This report describes the case of a 69-year-old woman with an esophageal tumor at the middle portion of the esophagus. The patient was successfully treated with minimal access thoracic surgery, and at the 6 month follow-up, the patient was free of any symptoms or recurrence.
- Published
- 2001
- Full Text
- View/download PDF
12. Bullatacin, a potent antitumor annonaceous acetogenin, inhibits proliferation of human hepatocarcinoma cell line 2.2.15 by apoptosis induction.
- Author
-
Chih HW, Chiu HF, Tang KS, Chang FR, and Wu YC
- Subjects
- Annexin A5 metabolism, Antineoplastic Agents, Phytogenic therapeutic use, Carcinoma, Hepatocellular drug therapy, Cell Death drug effects, Cell Division drug effects, Culture Media, Conditioned metabolism, DNA biosynthesis, DNA drug effects, Dose-Response Relationship, Drug, Ficoll analogs & derivatives, Ficoll metabolism, Flow Cytometry, Fluorescein-5-isothiocyanate analogs & derivatives, Fluorescein-5-isothiocyanate metabolism, Furans therapeutic use, Hepatitis B Surface Antigens metabolism, Humans, Liver Neoplasms drug therapy, Propidium metabolism, Tumor Cells, Cultured cytology, Tumor Cells, Cultured drug effects, Tumor Cells, Cultured pathology, Antineoplastic Agents, Phytogenic pharmacology, Apoptosis drug effects, Carcinoma, Hepatocellular pathology, Furans pharmacology, Liver Neoplasms pathology
- Abstract
Bullatacin, isolated from the fruit of Annona atemoya, is one of the most potentially effective antitumor annonaceous acetogenins. Bullatacin was studied here for its ability to inhibit the proliferation of 2.2.15 cells, hepatitis B virus (HBV) DNA transfected human hepatocarcinoma cell line. It was found that bullatacin induced cytotoxicity of 2.2.15 cells in a time- and dose-dependent manner. Fifty percent effective dose (ED50) on day 1 of exposure to bullatacin were 7.8 +/- 2.5 nM for 2.2.15 cells. [3H]-Thymidine incorporation assays showed almost the same results. Bullatacin-treatment also reduced concentrations of hepatitis B surface antigen (HBsAg) in the cultured medium released from 2.2.15 cells, coincident with the decrease in the cell proliferation. Analysis of mophological changes of bullatacin-treated 2.2.15 by inverted phase-contrast microscope and eletron microscopy revealed a possible model of action for bullatacin to inhibit proliferation of 2.2.15 cells by inducing apoptosis. Most of the bullatacin-induced cell death was found to be due to apoptosis, as determined by double staining with fluorescein-isothiocyanate (FITC)-labeled annexin V and propidium iodide (PI).
- Published
- 2001
- Full Text
- View/download PDF
13. Antiplatelet activity of N-methoxycarbonyl aporphines from Rollinia mucosa.
- Author
-
Kuo RY, Chang FR, Chen CY, Teng CM, Yen HF, and Wu YC
- Subjects
- Alkaloids isolation & purification, Aporphines isolation & purification, Humans, Magnetic Resonance Spectroscopy, Models, Molecular, Molecular Conformation, Molecular Structure, Platelet Aggregation drug effects, Platelet Aggregation Inhibitors chemistry, Platelet Aggregation Inhibitors isolation & purification, Spectrometry, Mass, Electrospray Ionization, Alkaloids chemistry, Alkaloids pharmacology, Aporphines chemistry, Aporphines pharmacology, Magnoliopsida chemistry, Platelet Aggregation Inhibitors pharmacology
- Abstract
Bioassay-directed fractionation of the stems of Rollinia mucosa led to the isolation of new N-methoxycarbonyl aporphine alkaloids, romucosine A (1), romucosine B (2), romucosine C (3), and romucosine D (4), along with the known alkaloid, N-methoxylcarbonyl-nornuciferine (5). Alkaloids 1 and 4 exhibited significant inhibition of collagen, arachidonic acid, and platelet activating factor-induced platelet aggregation, and alkaloid 3 also showed an inhibitory effect on arachidonic acid induced platelet aggregation.
- Published
- 2001
- Full Text
- View/download PDF
14. Four alkaloids from Annona cherimola.
- Author
-
Chen CY, Chang FR, Pan WB, and Wu YC
- Subjects
- Alkaloids isolation & purification, Medicine, Traditional, Methanol, Models, Molecular, Molecular Conformation, Molecular Structure, Taiwan, Alkaloids chemistry, Plant Extracts chemistry, Plants, Medicinal chemistry
- Abstract
Four alkaloids, annocherine A, annocherine B, cherianoine, and romucosine H, along with one known alkaloid, artabonatine B, were isolated from the MeOH extract of the stems of Annona cherimola. Their structures were identified on the basis of both analysis of their spectral data and from chemical evidence.
- Published
- 2001
- Full Text
- View/download PDF
15. Mechanisms for the formation of glycoxidation products in end-stage renal disease.
- Author
-
Weiss MF, Erhard P, Kader-Attia FA, Wu YC, Deoreo PB, Araki A, Glomb MA, and Monnier VM
- Subjects
- Adult, Aged, Aged, 80 and over, Arginine analogs & derivatives, Arginine blood, Enzyme Inhibitors pharmacology, Female, Glucose metabolism, Glycation End Products, Advanced blood, Guanidines pharmacology, Humans, Kidney Failure, Chronic blood, Kidney Failure, Chronic therapy, Lysine analogs & derivatives, Lysine blood, Maillard Reaction, Male, Middle Aged, Oxidation-Reduction, Peritoneal Dialysis, Renal Dialysis, Glycation End Products, Advanced biosynthesis, Kidney Failure, Chronic metabolism
- Abstract
Background: Advanced glycation end products (AGEs) accumulate on tissue and plasma proteins in patients with renal failure far in excess of normal aging or diabetes. The aim of these studies was to elucidate the nature of the precursors and the pathways that lead to an accelerated formation of two structurally identified AGEs [pentosidine and Nepsilon(carboxymethyl)lysine (CML)] in the uremic milieu., Methods: Serum levels of the glycoxidation products, pentosidine and CML, were quantitated by high-performance liquid chromatography in uremic patients treated by dialysis. The formation of early glycation products (as furosine) and late glycoxidation products was modeled in uremic serum and in spent peritoneal dialysate., Results: Clinical factors that affect circulating levels of AGEs included dialysis clearance and dialyzer membrane pore size, but not the presence or absence of diabetes. Both pentosidine and CML form at an accelerated rate in serum from uremic patients. Chelating agents most effectively slow the formation in vitro. In uremic fluids, the primary mechanism of formation of pentosidine is through the Amadori pathway. The primary mechanism of formation of CML is through metal-chelated autoxidation of reducing sugars generating reactive carbonyl precursors. In uremic serum, the presence of an unidentified reactive low molecular weight precursor accelerates the formation of pentosidine., Conclusions: The formation of the two glycoxidation products, pentosidine and CML, proceeds by different pathways and is enhanced by different precursors in the uremic milieu. The formation of both AGEs is markedly enhanced by metal-catalyzed reactions, evidence for the presence of increased metal-ion mediated oxidant stress in uremia.
- Published
- 2000
- Full Text
- View/download PDF
16. Aromin-A, an Annonaceous acetogenin from Annona cherimola.
- Author
-
Chen CY, Chang FR, Chiu HF, Wu MJ, and Wu YC
- Subjects
- Antineoplastic Agents, Phytogenic isolation & purification, Antineoplastic Agents, Phytogenic toxicity, Cell Survival drug effects, Chromatography, Thin Layer, Furans isolation & purification, Furans toxicity, Lactones isolation & purification, Lactones toxicity, Plant Stems, Tumor Cells, Cultured, Antineoplastic Agents, Phytogenic chemistry, Furans chemistry, Lactones chemistry, Plants, Medicinal
- Abstract
Chromatographic fractionation of a MeOH extract from the stems of Annona cherimola led to the isolation of a new non-adjacent, bis-THF ring acetogenin (one THF ring being at C-4 and the other at C-16), aromin-A (1) along with the known cytotoxic acetogenin squamocin (2). The structures of these isolates were established by means of mass spectrometry and spectroscopic techniques.
- Published
- 1999
- Full Text
- View/download PDF
17. Determination of hydroxyaromatic compounds in water by solid-phase microextraction coupled to high-performance liquid chromatography.
- Author
-
Wu YC and Huang SD
- Subjects
- Adsorption, Chromatography, High Pressure Liquid, Hydrogen-Ion Concentration, Hydroxylation, Osmolar Concentration, Spectrophotometry, Ultraviolet, Temperature, Fresh Water analysis, Hydrocarbons, Aromatic analysis, Water Pollutants, Chemical analysis
- Abstract
Solid-phase microextraction (SPME) coupled with high-performance liquid chromatography (HPLC) for the analysis of hydroxyaromatic compounds is described. Three kinds of fibers [50 microns carbowax-templated resin (CW-TPR), 60 microns polydimethylsiloxane-divinylbenzene (PDMS-DVB) and 85 microns polyacrylate (PA) fibers] were evaluated. CW-TPR and PDMS-DVB were selected for further study. The parameters of the desorption procedure (such as desorption mode, the composition of the solvent for desorption and the duration of fiber soaking) were studied and optimized. The effect of the structure and physical properties of analytes, carryover, duration of absorption, temperature of absorption, pH and ionic strength of samples were also investigated. The method was applied to environmental samples (lake water) using a simple calibration curve.
- Published
- 1999
- Full Text
- View/download PDF
18. Rhinacanthin-Q, a naphthoquinone from Rhinacanthus nasutus and its biological activity.
- Author
-
Wu TS, Hsu HC, Wu PL, Teng CM, and Wu YC
- Subjects
- Humans, KB Cells drug effects, Naphthoquinones chemistry, Nuclear Magnetic Resonance, Biomolecular, Plant Extracts chemistry, Plant Roots chemistry, Platelet Aggregation drug effects, Platelet Aggregation Inhibitors pharmacology, Naphthoquinones isolation & purification, Naphthoquinones pharmacology, Plant Extracts isolation & purification, Plant Extracts pharmacology, Plants, Medicinal chemistry
- Abstract
The continuing investigation of the root of Rhinacanthus nasutus afforded a 1,4-naphthoquinone ester, rhinacanthin-Q, accompanied by twenty-four known compounds. The structure was elucidated on the basis of spectroscopic analyses. The cytotoxicity and antiplatelet effect of this compound was also discussed.
- Published
- 1998
- Full Text
- View/download PDF
19. Two new 7-dehydroaporphine alkaloids and antiplatelet action aporphines from the leaves of Annona purpurea.
- Author
-
Chang FR, Wei JL, Teng CM, and Wu YC
- Subjects
- Alkaloids chemistry, Aporphines chemistry, Chemical Phenomena, Chemistry, Physical, Humans, Plant Leaves chemistry, Platelet Activating Factor pharmacology, Platelet Aggregation drug effects, Platelet Aggregation Inhibitors chemistry, Spectrophotometry, Trees, Alkaloids isolation & purification, Alkaloids pharmacology, Aporphines isolation & purification, Aporphines pharmacology, Plants, Medicinal chemistry, Platelet Aggregation Inhibitors isolation & purification, Platelet Aggregation Inhibitors pharmacology
- Abstract
Bioactivity-directed fractionation led to the isolation of two new 7-dehydroaporphine alkaloids, 7-hydroxy-dehydrothalicsimidine (1) and 7-formyl-dehydrothalicsimidine (2), along with the five known alkaloids, thalicsimidine (3), norpurpureine (4), N-methyllaurotetanine (5), lirinidine (6) and N-methylasimilobine (7), from the leaves of Annona purpurea. Structural elucidation of these compounds was established by mass and spectroscopic analyses. Among them, 1, 3, 4, 6 and 7 exhibited significant inhibition of collagen, arachidonic acid and platelet activating factor-induced platelet aggregation; 1 also showed inhibition against thrombin-induced platelet aggregation.
- Published
- 1998
- Full Text
- View/download PDF
20. Acetogenins from seeds of Annona reticulata.
- Author
-
Chang FR, Chen JL, Chiu HF, Wu MJ, and Wu YC
- Subjects
- Antineoplastic Agents, Phytogenic isolation & purification, Antineoplastic Agents, Phytogenic toxicity, Carcinoma, Hepatocellular, Colonic Neoplasms, Furans isolation & purification, Furans toxicity, Humans, KB Cells, Lactones isolation & purification, Lactones toxicity, Tumor Cells, Cultured, Antineoplastic Agents, Phytogenic chemistry, Cell Survival drug effects, Furans chemistry, Lactones chemistry, Plants, Medicinal, Seeds
- Abstract
Chromatography of an ethyl acetate extract of seeds of Annona reticulata led to the isolation of a new cytotoxic gamma-lactone acetogenin, cis-/trans-isomurisolenin, along with six known cytotoxic acetogenins, annoreticuin, annoreticuin-9-one, bullatacin, squamocin, cis-/trans-bullatacinone and cis-/trans-murisolinone. Structures of these compounds were established by means of mass and related spectral experiments. Some of the compounds isolated, showed potent cytotoxicities against Hep. 2,2,15, Hep. G2, KB and CCM2, four cancer cell-lines.
- Published
- 1998
- Full Text
- View/download PDF
21. Post mass-immunization measles outbreak in Taoyuan County, Taiwan: dynamics of transmission, vaccine effectiveness, and herd immunity.
- Author
-
Lee MS, Lee LL, Chen HY, Wu YC, and Horng CB
- Subjects
- Antibodies, Viral blood, Child, Child, Preschool, Humans, Immunoglobulin G blood, Immunoglobulin M blood, Infant, Measles prevention & control, Measles transmission, Measles Vaccine administration & dosage, Risk Factors, Seroepidemiologic Studies, Taiwan epidemiology, Vaccines, Attenuated administration & dosage, Vaccines, Attenuated immunology, Disease Outbreaks, Measles epidemiology, Measles Vaccine immunology
- Abstract
Objectives: Analysis of national surveillance data and a seroepidemiologic investigation were conducted to elucidate the causes and epidemiologic characteristics of a measles outbreak in Taoyuan, Taiwan, 1994., Methods: Measles cases were identified through a national surveillance system. Reported cases and their physician or school nurses were interviewed to trace additional suspect cases and were sampled for serologic diagnosis. Measles-specific IgG and IgM were assayed. A confirmed case was defined as being positive for measles IgM test but not having received measles vaccination within the previous 3 months., Results: The outbreak began in Taoyuan City in December 1993 and continued to spread in primary schools and kindergartens, but caused only sporadic cases in neighboring towns. Among 42 confirmed cases, 15 (38%) were primary school children and 16 (38%) were kindergarten children. Among 24 confirmed cases with a vaccination record, 7 had one dose of vaccination, 4 had two doses of vaccination, and 13 (54%) were unvaccinated. The overall measles susceptible proportion at a kindergarten before the outbreak was 8.1% (17/209) and the overall measles cumulative incidence among susceptibles was 0.65 (11/17)., Conclusions: A measles vaccination coverage of 82% with the first dose at 9 months of age and 63% with the second dose (measles, mumps, and rubella) at 15 months was inadequate to block measles virus circulation in Taoyuan City in 1994. The city center, with a growing population, represents a high risk as an epicenter for measles outbreaks. Measles outbreaks may occur in a school population with 92% herd immunity.
- Published
- 1998
- Full Text
- View/download PDF
22. Minimally invasive approach for coronary artery bypass surgery.
- Author
-
Wu YC, Chang CH, Lin PJ, Chu JJ, Tsai FC, Yang MW, and Tan PP
- Subjects
- Adult, Aged, Aged, 80 and over, Aorta surgery, Coronary Angiography, Coronary Disease diagnosis, Echocardiography, Extracorporeal Circulation, Female, Humans, Length of Stay, Male, Middle Aged, Saphenous Vein transplantation, Thoracic Arteries transplantation, Thoracotomy methods, Treatment Outcome, Vascular Patency, Coronary Artery Bypass methods, Coronary Disease surgery, Minimally Invasive Surgical Procedures
- Abstract
Forty-two patients, 33 male and 9 female, aged 35.7 to 81.6 years old (mean 62.7), were operated on for left main and/or triple vessel coronary artery disease by using minimally invasive cardiac surgical techniques. A myocardial infarction had occurred in 26 patients (61.9%). The left ventricular ejection fraction ranged from 17 to 83% (52+/-22). The surgeries were performed through left parasternal minithoracotomy (8 to 12 cm in length) under femoro-femoral or aorto-atrial cardiopulmonary bypass. The myocardium was protected by blood cardioplegic solution with the aorta crossclamped. Under direct vision, average 3.8 distal anastomoses were performed in each patient, with the saphenous vein grafts and the left internal thoracic arterial graft. The aortic crossclamp time was 62 to 137 min (80+/-15). The duration of cardiopulmonary bypass was 88 to 168 min (115+/-24). The postoperative course was uneventful in all patients. Follow-up (1.0 to 5.6 months, mean 2.9) was complete in all patients and there were no late deaths or angina. Coronary angiography of ten patients showed patent grafts. Our experience demonstrates that minimally invasive cardiac surgery during cardioplegic arrest is technically feasible and can be performed in coronary artery disease safely and effectively for complete revascularization.
- Published
- 1997
- Full Text
- View/download PDF
23. Primary pulmonary malignant hemangiopericytoma associated with coagulopathy.
- Author
-
Wu YC, Wang LS, Chen W, Fahn HJ, Huang MH, and Whang-Peng J
- Subjects
- Adult, Antifibrinolytic Agents blood, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Blood Coagulation Tests, Blood Loss, Surgical, Female, Fibrin Fibrinogen Degradation Products analysis, Fibrinogen analysis, Fibrinolysis, Hemangiopericytoma drug therapy, Hemangiopericytoma pathology, Hemangiopericytoma surgery, Humans, Lung Neoplasms drug therapy, Lung Neoplasms pathology, Lung Neoplasms surgery, Neoplasm Recurrence, Local pathology, Pneumonectomy, Postoperative Hemorrhage etiology, Thrombophlebitis etiology, Blood Coagulation Disorders etiology, Hemangiopericytoma complications, Lung Neoplasms complications, Paraneoplastic Syndromes etiology
- Abstract
A 34-year-old woman with a massive pulmonary malignant hemangiopericytoma and coagulopathy as a paraneoplastic syndrome is reported. Although coagulopathy may appear as a paraneoplastic syndrome and cause treatment to be more complicated and difficult, it can also be a useful marker to monitor the results of operation and tumor recurrence. This unusual case shows that primary pulmonary malignant hemangiopericytoma deserves aggressive surgical intervention with complete resection even under the circumstances of coagulation abnormality.
- Published
- 1997
- Full Text
- View/download PDF
24. Reliable determination of furosine in human serum and dialysate proteins by high-performance liquid chromatography.
- Author
-
Wu YC, Monnier V, and Friedlander M
- Subjects
- Borohydrides pharmacology, Chromatography, High Pressure Liquid statistics & numerical data, Glucose analysis, Humans, Kidney Failure, Chronic blood, Kidney Failure, Chronic therapy, Kinetics, Lysine blood, Peritoneal Dialysis, Chromatography, High Pressure Liquid methods, Dialysis Solutions chemistry, Glycoproteins analysis, Lysine analogs & derivatives
- Abstract
Furosine, formed by hydrolysis of 1-deoxy-fructosyl-lysine (fructose-lysine), is a product of the Amadori rearrangement of glucose and epsilon-NH2-lysine. Fructose-lysine can react further with tissue and circulating proteins to produce advanced glycation end-products (AGEs). Peritoneal dialysate used in the treatment of patients with end-stage renal failure contains high concentrations of glucose which may lead to intraperitoneal formation of AGEs. To quantitate the kinetics of formation and peritoneal clearance of glycated peritoneal dialysate proteins, we developed an effective approach to the measurement of furosine in clinical samples of serum and peritoneal dialysate.
- Published
- 1995
- Full Text
- View/download PDF
25. Constituents and cytotoxic principles of Nothapodytes foetida.
- Author
-
Wu TS, Leu YL, Hsu HC, Ou LF, Chen CC, Chen CF, Ou JC, and Wu YC
- Subjects
- Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic pharmacology, Camptothecin chemistry, Camptothecin isolation & purification, Camptothecin pharmacology, Drug Screening Assays, Antitumor, Humans, Magnetic Resonance Spectroscopy, Spectrophotometry, Ultraviolet, Tumor Cells, Cultured, Antineoplastic Agents, Phytogenic isolation & purification, Camptothecin analogs & derivatives, Trees chemistry
- Abstract
A new naturally occurring alkaloid, acetylcamptothecin, together with 17 known compounds, (+)-1-hydroxypinoresinol, omega-hydroxypropioguaiacone, p-hydroxybenzaldehyde, scopoletin, uracil, thymine, sitosterol, sitosteryl-beta-D-glucoside, 3 beta-hydroxy-stigmast-5-en-7-one, stigmast-5-en-3 beta,7 alpha-diol, 6 beta-hydroxystigmast-4-en-3-one, sitost-4-en-3-one, linoleic acid, trigonelline, camptothecin, 9-O-methoxycamptothecin and pumiloside were isolated and characterized from the stem of Nothapodytes foetida. Among them, scopoletin, camptothecin, 9-O-methoxycamptothecin and O-acetylcamptothecin showed significant cytotoxic activity.
- Published
- 1995
- Full Text
- View/download PDF
26. Disparate cytochemical characteristics and production of cytokines and prostaglandin E2 by human mononuclear phagocytes from the blood, lung, and peritoneal cavity.
- Author
-
Friedlander MA, Hilbert CM, Wu YC, Finegan CK, and Rich EA
- Subjects
- Adult, Biological Assay, Cytoplasm enzymology, Enzyme-Linked Immunosorbent Assay, Esterases metabolism, Humans, Interleukin-1 biosynthesis, Peroxidases metabolism, Radioimmunoassay, Reference Values, Tumor Necrosis Factor-alpha biosynthesis, Cytokines biosynthesis, Dinoprostone biosynthesis, Macrophages, Alveolar metabolism, Macrophages, Peritoneal metabolism, Monocytes metabolism
- Abstract
The cytochemical characteristics and the production of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and prostaglandin E2 (PGE2) by peritoneal macrophages were compared with those of blood monocytes and alveolar macrophages. The comparative percentages of mononuclear phagocytes positive for peroxidase were as follows: blood monocytes > peritoneal macrophages > alveolar macrophages. The comparative percentages of cells positive for nonspecific esterase were as follows: alveolar macrophages > peritoneal macrophages = blood monocytes. The intensity of staining for nonspecific esterase was highest in alveolar macrophages and lowest in blood monocytes. Constitutive release of TNF, IL-1 beta, and PGE2 was minimal by each cell type. Lipopolysaccharide-stimulated TNF production by alveolar macrophages was approximately five times greater than that of monocytes and 10 times greater than that of peritoneal macrophages. By contrast, lipopolysaccharide-stimulated blood monocytes produced significantly more IL-1 beta than did peritoneal or alveolar macrophages. Lipopolysaccharide-stimulated production of PGE2 by peritoneal macrophages was significantly less than that of alveolar macrophages or blood monocytes. Thus peritoneal macrophages release relatively low levels of IL-1 beta, TNF, and PGE2 in response to lipopolysaccharide. Peritoneal and alveolar macrophages differ with respect to both cytochemical characteristics and lipopolysaccharide-stimulated production of TNF and PGE2 but are similar in their limited capacity to produce IL-1 beta.
- Published
- 1994
27. Combined nasal intermittent positive-pressure ventilation and rocking bed in chronic respiratory insufficiency. Nocturnal ventilatory support of a disabled person at home.
- Author
-
Goldberg AI, Cane RD, Childress D, Wu YC, Vesely LC, and Pfrommer M
- Subjects
- Carbon Dioxide metabolism, Chronic Disease, Electric Power Supplies, Equipment Design, Female, Humans, Inspiratory Capacity, Intermittent Positive-Pressure Ventilation instrumentation, Middle Aged, Oxygen blood, Pulse, Respiration, Respiratory Insufficiency physiopathology, Respiratory Muscles physiopathology, Sleep, Tidal Volume, Vital Capacity, Beds, Intermittent Positive-Pressure Ventilation methods, Masks, Quadriplegia physiopathology, Respiratory Insufficiency therapy
- Abstract
The use of intermittent positive-pressure ventilation via nasal mask with a rocking bed provided the necessary ventilatory support for a person with quadriplegia living at home. This option was required to maintain an independent life-style of choice. The combination of techniques and the linkage of devices demonstrated an effective use of simple available technology for respiratory care at home and the adaptation of the respiratory prescription to the total needs of each person: medical, social, and financial. The further use of nasal mask ventilation in selected cases is encouraged by clinical research protocol.
- Published
- 1991
- Full Text
- View/download PDF
28. Opiate binding to cerebroside sulfate: a model system for opiate-receptor interaction.
- Author
-
Loh HH, Cho TM, Wu YC, Harris RA, and Way EL
- Subjects
- Animals, Liposomes, Mice, Mice, Inbred CBA, Mice, Inbred ICR, Models, Biological, Morphine metabolism, Mutation, Structure-Activity Relationship, Sulfoglycosphingolipids metabolism, Cerebrosides metabolism, Etorphine metabolism, Levorphanol metabolism, Morphinans metabolism, Receptors, Drug
- Published
- 1975
- Full Text
- View/download PDF
29. Stereospecific binding of narcotics to brain cerebrosides.
- Author
-
Loh HH, Cho TM, Wu YC, and Way EL
- Subjects
- Analgesics metabolism, Animals, Binding Sites, Cattle, Chromatography, Ion Exchange, Chromatography, Thin Layer, Dextromethorphan pharmacology, Fluorescence, Kinetics, Levorphanol pharmacology, Mice, Morphine pharmacology, Narcotics pharmacology, Nerve Tissue Proteins metabolism, Pentanols metabolism, Protein Binding, Sulfoglycosphingolipids metabolism, Tritium, Brain metabolism, Cerebrosides metabolism, Morphinans metabolism, Naloxone metabolism, Narcotic Antagonists metabolism, Receptors, Drug
- Published
- 1974
- Full Text
- View/download PDF
30. Polyamide-silica gel thin-layer chromatography of water-soluble vitamins.
- Author
-
Chiang HC, Lin Y, and Wu YC
- Subjects
- Ascorbic Acid analysis, Chromatography, Thin Layer, Orotic Acid analysis, Rutin analysis, Vitamin B Complex analysis, Vitamins analysis
- Published
- 1969
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.