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Foamed concrete has a good energy absorption capability and can be used as seismic isolation material for tunnels. This study aims to investigate the mechanical properties and associated seismic isolation effects of foamed concrete layer in rock tunnel. For this, a series of uniaxial/triaxial compression tests was conducted to understand the effects of concrete density, confining stress and strain rate on the mechanical properties of foamed concrete. The direct shear tests were also performed to investigate the effects of concrete density and normal stress on the nonlinear behaviors of foamed concrete layer-lining interface. The test results showed that the mechanical properties of foamed concrete are significantly influenced by the concrete density. The foamed concrete also has high volumetric compressibility and strain-rate dependence. The peak stress, residual stress, shear stiffness and residual friction coefficient of the foamed concrete layer-lining interface are influenced by the foamed concrete density and normal stress applied. Then, a crushable foam constitutive model was constructed using ABAQUS software and a composite exponential model was also established to study the relationship between shear stress and shear displacement of the interface, in which their parameters were fitted based on the experimental results. Finally, a parametric analysis using the finite element method (FEM) was conducted to understand the influence of foamed concrete layer properties on the seismic isolation effect, including the density and thickness of the layer as well as the shear stiffness and residual friction coefficient of the interface. It was revealed that lower density and greater thickness in addition to smaller shear stiffness or residual friction coefficient of the foamed concrete layer could yield better seismic isolation effect, and the influences of the first two tend to be more significant. Keywords: Rock tunnel, Foamed concrete, Seismic isolation layer, Seismic isolation mechanism