1. Transcriptomic and metabolomic insights into the variety of sperm storage in oviduct of egg layers
- Author
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Ge Yang, Shaomei Li, Qianqian Zhao, Jinyu Chu, Baogui Zhou, Shijie Fan, Fengying Shi, Xiaoran Wei, Xuewen Hu, Xinting Zheng, Zhiwei Liu, Xiaoliu Zhou, Yingfeng Tao, Shijun Li, and Chunyan Mou
- Subjects
transcriptome ,metabolome ,sperm storage ,uterovaginal junction ,chicken ,Animal culture ,SF1-1100 - Abstract
In birds, the sperm storage tubules (SST) are dispersed in uterovaginal junction (UVJ) and highly correlated with differential capacity of sperm storage (SS) in and among species with unspecified mechanisms. Here, the SS duration of 252 egg layer breeders was evaluated in 5 rounds with 3 phenotypic traits to screen high- and low-SS individuals, respectively, followed with transcriptome of UVJ tissues and metabolome of serum (high-SS vs. low-SS) to decipher the candidate genes and biochemical markers correlated with differential SS capacity. Histological characterization suggested slightly higher density of SST in UVJ (high-SS vs. low-SS). Transcriptome analyses identified 596 differentially expressed genes (336 upregulated vs. 260 downregulated), which were mainly enriched in gene ontology terms of homeostasis, steroid and lipid metabolism and hormone activity, and 12 significant pathways (P
- Published
- 2021
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