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The reduction of selenite [Se(Ⅳ)] by microorganisms is a green and efficient detoxification strategy. We found that Se(Ⅳ) inhibited exopolysaccharide and protein secretion by Lactiplantibacillus plantarum BSe and compromised cell integrity. In this study, L. plantarum BSe reduced Se(Ⅳ) by increasing related enzyme activity and electron transfer. Genomic analysis demonstrated that L. plantarum BSe should be able to reduce Se(Ⅳ). Further transcriptome analysis showed that L. plantarum BSe enhanced its tolerance to Se(Ⅳ) by upregulating the expression of surface proteins and transporters, thus reducing the extracellular Se(Ⅳ) concentration through related enzymatic reactions and siderophore-mediated pathways. Lactiplantibacillus plantarum BSe was able to regulate the expression of related genes involved in quorum sensing and a two-component system and then select appropriate strategies for Se(Ⅳ) transformation in response to varying environmental Se(Ⅳ) concentrations. In addition, azo reductase was linked to the reduction of Se(Ⅳ) for the first time. The present study established a multipath model for the reduction of Se(Ⅳ) by L. plantarum, providing new insights into the biological reduction of Se(Ⅳ) and the biogeochemical cycle of selenium., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Monitoring the levels of perfluorinated compounds (PFCs) in the environment is of vital importance, owing to their sustained environmental presence, extensive distribution, and associated health risks. The development of cost-effective and efficient sorbents for the establishment of sensitive analytical methods is critical for achieving trace-level detection. In this study, a graphitic carbon nitride (g-C 3 N 4 )-based sorbent is synthesized by a facile sonication-assisted method exfoliated by zeolitic imidazolate framework-67 (ZIF-67) in situ. The novel ZIF-67/g-C 3 N 4 composites were systematically characterized by transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, thermogravimetric analysis, and N 2 adsorption-desorption analysis, exhibiting good dispersity and a large surface area. Moreover, molecular dynamics simulations indicated that g-C 3 N 4 structures can be effectively exfoliated by the introduced ZIF-67 molecules. The hybrid material was successfully utilized as a dispersive solid-phase extraction sorbent, and the extraction factors were systematically optimized by response surface methodology. Under optimal conditions, the synthesized sorbent exhibited desirable linear correlations (R 2 > 0.99), a low detection limit (0.3-2 ng L -1 ), and good repeatability (relative standard deviation <15%, n = 6). The developed method was applied for the analysis of natural and spiked water samples. The study demonstrated that the ZIF-67/g-C 3 N 4 composites are promising materials for pollutant adsorption from drinking water samples., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020. Published by Elsevier B.V.)

Lambda-cyhalothrin is a pyrethroid widely used in crop, fruit and vegetable production, but has potential health threats to human. Immunoassay is a cheap, rapid and facile method to detect lambda-cyhalothrin, yet wide application of this method still requires improvement in the construction of antigen. In this study, we developed a one-step lambda-cyhalothrin hapten synthesis that transformed the cyanide group in lambda-cyhalothrin to amide. Complete antigen was assembled by coupling the amide with succinic-anhydride-activated carrier proteins, and corresponding polyclonal antibodies were generated using Balb/c mice. Using antibody generated by the method in this paper, the competitive ELISA demonstrated the lowest detection limit of 3.772 μg/L for lambda-cyhalothrin, and no significant cross-reactivity for other pyrethroid pesticides was observed. All the results suggested we have established a more efficient technique of generating lambda-cyhalothrin antibody. Furthermore, since the activated proteins used in this study are highly controllable, we believe these proteins could potentially be the prototype of a series of standardized carrier proteins for the synthesis of complete antigens., Competing Interests: Declaration of competing interest The authors have filed a patent application covering the synthesis of hapten and protein activation: Chinese patent application no. 201911291688.2., (Copyright © 2020 Elsevier Inc. All rights reserved.)