22 results on '"Zhao, Guang-hui"'
Search Results
2. Treatment and prevention
- Author
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Zhang, Long-Xian, primary, Wang, Rong-Jun, additional, Zhao, Guang-Hui, additional, and Li, Jun-Qiang, additional
- Published
- 2021
- Full Text
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3. Epidemiology in human and animals
- Author
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Zhang, Long-Xian, primary, Wang, Rong-Jun, additional, Zhao, Guang-Hui, additional, and Li, Jun-Qiang, additional
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- 2021
- Full Text
- View/download PDF
4. Molecular characteristics
- Author
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Zhang, Long-Xian, primary, Wang, Rong-Jun, additional, Zhao, Guang-Hui, additional, and Li, Jun-Qiang, additional
- Published
- 2021
- Full Text
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5. Conclusions and perspective
- Author
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Zhang, Long-Xian, primary, Wang, Rong-Jun, additional, Zhao, Guang-Hui, additional, and Li, Jun-Qiang, additional
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- 2021
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6. Taxonomy and biology
- Author
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Zhang, Long-Xian, primary, Wang, Rong-Jun, additional, Zhao, Guang-Hui, additional, and Li, Jun-Qiang, additional
- Published
- 2021
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7. Transmission risk factors
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Zhang, Long-Xian, primary, Wang, Rong-Jun, additional, Zhao, Guang-Hui, additional, and Li, Jun-Qiang, additional
- Published
- 2021
- Full Text
- View/download PDF
8. Clinical feature
- Author
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Zhang, Long-Xian, primary, Wang, Rong-Jun, additional, Zhao, Guang-Hui, additional, and Li, Jun-Qiang, additional
- Published
- 2021
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9. MiR-4521 affects the propagation of Cryptosporidium parvum in HCT-8 cells through targeting foxm1 by regulating cell apoptosis.
- Author
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Yao Q, Fan YY, Huang S, Hu GR, Song JK, Yang X, and Zhao GH
- Subjects
- Humans, Apoptosis genetics, Cryptosporidiosis genetics, Cryptosporidiosis pathology, Cryptosporidium parvum genetics, MicroRNAs genetics, Forkhead Box Protein M1 genetics
- Abstract
Cryptosporidium parvum could regulate the expression of microRNAs of epithelial cells to facilitate its intracellular propagation. MiR-4521 has been reported to play an important role during the development and progression of tumors and infectious diseases by regulating cell proliferation, apoptosis, and autophagy. However, the implication of miR-4521 during C. parvum infection was still unknown. In this study, the expression of miR-4521 was found to be upregulated in HCT-8 cells infected with C. parvum from 8 h post-infection (pi) to 48 hpi, and its upregulation would be related with the TLR/NF-κB signal pathway during C. parvum infection. One potential target of miR-4521, foxm1, was down-regulated in HCT-8 cells from 24 hpi to 48 hpi, and the expression of foxm1 was negatively regulated by miR-4521. The target relationship between miR-4521 and foxm1 was further validated by using dual luciferase reporter assay. Further studies showed that miR-4521 promoted the propagation of C. parvum in HCT-8 cells through targeting foxm1 by regulating BCL2-mediating cell apoptosis. These results contribute to further understanding of the regulatory mechanisms of host miRNAs during Cryptosporidium infection., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest.., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2024
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10. Circular RNA ciRS-7 affects the propagation of Cryptosporidium parvum in HCT-8 cells via regulating miR-135a-5p/stat1 axis.
- Author
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Yin YL, Yang X, Huang S, Hu GR, Yao Q, Song JK, and Zhao GH
- Subjects
- Animals, Humans, Cell Line, Tumor, RNA, Circular genetics, STAT1 Transcription Factor genetics, STAT1 Transcription Factor metabolism, Cryptosporidiosis genetics, Cryptosporidium genetics, Cryptosporidium parvum genetics, MicroRNAs genetics
- Abstract
Cryptosporidium spp. are protozoan parasites that mainly inhabit intestinal epithelial cells, causing diarrheal diseases in humans and a great number of animals. Cryptosporidium parvum is the most common zoonotic species, responsible for nearly 45% of human cryptosporidiosis worldwide. Understanding the interaction mechanisms between C. parvum and host gastrointestinal epithelial cells has significant implications to control cryptosporidiosis. One up-regulated circRNA ciRS-7 was found previously by our group to promote in vitro propagation of C. parvum in HCT-8 cells. In the present study, miR-135a-5p, was found to be a miRNA target of ciRS-7. Cryptosporidium parvum infection induced significantly down-regulation of miR-135a-5p and dramatic up-regulation of its potential target stat1 gene at mRNA and protein levels. Dual luciferase reporter assays validated the physical interactions between miR-135a-5p and stat1, and between ciRS-7 and miR-135a-5p. Further study revealed that ciRS-7 could sponge miR-135a-5p to positively regulate the protein levels of STAT1 and phosphorylated STAT1 (p-STAT1) and thus promote C. parvum propagation in HCT-8 cells. Our findings further reveal the mystery of regulatory roles of host circRNAs during Cryptosporidium infection, and provide a novel insight to develop strategies to control cryptosporidiosis., Competing Interests: Declaration of Competing Interest Authors declare no conflict of interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
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11. Neospora caninum infection activated autophagy of caprine endometrial epithelial cells via mTOR signaling.
- Author
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Zhao SS, Tao DL, Chen JM, Chen X, Geng XL, Wang JW, Yang X, Song JK, Liu Q, and Zhao GH
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- Animals, Autophagy, Cattle, Epithelial Cells, Female, Goats, Pregnancy, Sirolimus, TOR Serine-Threonine Kinases, Cattle Diseases, Coccidiosis veterinary, Goat Diseases, Neospora
- Abstract
Neosporosis, caused by infection with the protozoan parasite Neospora caninum, is one of the main causes of abortion in cattle and small ruminants (e.g., goats), negatively influencing animal health and production costs. The uterus is an adhesion organ of placenta that is important for pregnancy and embryonic development. However, the underlying molecular pathogenic mechanisms of N. caninum in the uterus are still unclear. Autophagy regulates innate and adaptive immunity for eliminating pathogens by xenophagy, while pathogens can manipulate autophagy to facilitate their propagation. To study the role of host cell autophagy during N. caninum infection, a N. caninum infection model in caprine endometrial epithelial cells (EECs) was successfully established. In this in vitro model, N. caninum infection increased the expression of LC3-II (a standard marker for autophagosomes) from 6 h post infection (pi) to 48 h pi and the number of autophagosomes in caprine EECs at 48 h pi. Expression of p62 protein (a classical receptor of autophagy) levels were significantly decreased (P < 0.05) in caprine EECs infected with N. caninum tachyzoites at both 24 h pi and 48 h pi. Enhanced autophagic flux was also detected at 48 h pi in caprine EECs infected with N. caninum tachyzoites by transfecting Ad-mCherry-GFP-LC3B recombinant adenovirus. Treatments using a mechanistic target of rapamycin (mTOR)-specific inhibitor (rapamycin) and an autophagy inhibitor (chloroquine) indicated that cell autophagy induced by N. caninum infection promoted the intracellular propagation of parasite tachyzoites. Further studies showed that N. caninum infection induced autophagy through inhibition of mTOR phosphorylation. To the best of our current knowledge, this is the first study to reveal the role of autophagy during N. caninum infection in caprine EECs, and the findings provided significant information for uncovering mechanisms of abortion and pathogenicity caused by N. caninum infection., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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12. Micro-RNA expression profile of chicken small intestines during Eimeria necatrix infection.
- Author
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Liu TL, Fan XC, Wang Y, Wang YX, Wang JW, Song JK, and Zhao GH
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- Animals, Coccidiosis genetics, Coccidiosis metabolism, Coccidiosis parasitology, Eimeria physiology, Gene Expression Profiling veterinary, Intestine, Small metabolism, Intestine, Small parasitology, Poultry Diseases metabolism, Poultry Diseases parasitology, RNA, Messenger metabolism, Chickens, Coccidiosis veterinary, Poultry Diseases genetics, RNA, Messenger genetics, Transcriptome
- Abstract
Eimeria necatrix is a high pathogenic pathogen second to Eimeria tenella causing chicken coccidiosis. However, the precise underlying molecular mechanisms of interaction between E. necatrix and chickens are not fully understood. Accumulating evidences suggest that micro-RNAs (miRNAs) play pivotal regulatory roles in various diseases, including parasitic diseases. In the present study, the expression profile of miRNAs in Hy-line variety white chicken small intestines infected with E. necatrix was studied by using deep sequencing. A total of 35 miRNAs (including 16 significantly upregulated and 19 significantly downregulated miRNAs) were significantly differentially expressed (DE) in infected tissues at 108 h post-infection (pi). Real-time polymerase chain of 10 miRNAs (including 5 upregulated and 5 downregulated) randomly selected successfully confirmed the effectiveness of deep sequencing. Target prediction showed that 4,568 mRNAs could be regulated by 21 (including 12 upregulated and 9 downregulated) of 35 differentially expressed miRNAs. Functional analysis indicated that target genes of these differentially expressed miRNAs would be involved in pathways related to infection of E. necatrix, including cell differentiation, adhesion, proliferation, and apoptosis (e.g., MAPK signaling pathway and PPAR signaling pathway). To our best knowledge, this is the first study on the miRNA expression profile of small intestines during E. necatrix infection, and the findings in the present study suggested that these DE miRNAs would play important regulatory role in the interaction between E. necatrix and chicken intestines., (Copyright © 2020. Published by Elsevier Inc.)
- Published
- 2020
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13. Prevalence and genotypes of Enterocytozoon bieneusi in China.
- Author
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Wang SS, Wang RJ, Fan XC, Liu TL, Zhang LX, and Zhao GH
- Subjects
- Animals, China epidemiology, DNA, Fungal genetics, DNA, Ribosomal Spacer genetics, Enterocytozoon growth & development, Genetic Variation, Genotype, Humans, Microsporidiosis microbiology, Multilocus Sequence Typing, Phylogeny, Prevalence, Enterocytozoon genetics, Microsporidiosis epidemiology, Microsporidiosis genetics
- Abstract
Enterocytozoon bieneusi has been considered as the most frequently diagnosed microsporidian species in humans and various animal species, accounting for more than 90% of the cases of human microsporidiosis. Spores of this pathogen excreted from both symptomatic and asymptomatic hosts into environment also would be an important source of waterborne outbreak of microsporidiosis. Due to limited effective drugs available but with too much side effects to mammals (eg. toxic), accurate characterization of E. bieneusi in both humans and animals is essential to implement effective control strategies to this pathogen. In China, E. bieneusi infection was presented in humans and some animals with high prevalence. Analysis of genetic variations of the internal transcribed spacer (ITS) sequences found 361 genotypes in China, and some novel genotypes were identified in some specific hosts. Additionally, associations between infections and some risk factors were also observed. In the present article, we reviewed the current status of prevalence, genotypes, multilocus genotypes (MLGs) in humans, various animals and waters in China. These findings will provide basic information for developing effective control strategies against E. bieneusi infection in China as well as other countries., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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14. Prevalence and multi-locus genotyping of Giardia duodenalis from goats in Shaanxi province, northwestern China.
- Author
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Yin YL, Zhang HJ, Yuan YJ, Tang H, Chen D, Jing S, Wu HX, Wang SS, and Zhao GH
- Subjects
- Animals, China epidemiology, Genetic Variation, Genotype, Giardia lamblia isolation & purification, Giardiasis epidemiology, Giardiasis veterinary, Prevalence, Triose-Phosphate Isomerase genetics, Giardia lamblia genetics, Goats parasitology, Multilocus Sequence Typing
- Abstract
Giardia duodenalis (syn. Giardia lamblia, Giardia intestinalis) is an important zoonotic parasite that parasitizes the gastro-intestines of humans and animals, with diarrhea as the most common clinical symptom. The goat has been indicated as one of the most important reservoirs of G. duodenalis for humans. In the present study, we investigated the prevalence and genetic diversity of G. duodenalis in goats in Shaanxi province, northwestern China. A total of 1311 faecal specimens were examined, and the overall prevalence was 7.1% (93/1311). Although all the meat, cashmere and dairy goats were positive for infection, the highest prevalence was found in cashmere goats (10.2%), followed by dairy (9.4%) and meat goats (2.0%). Negative correlation between age and prevalence was also observed, and the highest prevalence was detected in 0-2-month goats. Genetic analysis showed the presence of three assemblages, including two zoonotic (A and B) and one animal-adapted assemblage E, with E as the prevalent assemblage found in all breeds of positive goats. The zoonotic assemblage A was found in Guanzhong dairy and Shanbei cashmere goats, but B was only detected in Boar goats. Additionally, mixed assemblages E and A were also identified in two cashmere goats. Multi-locus genotyping (MLST) using the gene loci of the triosephosphate isomerase (tpi), b-giardin (bg) and glutamate dehydrogenase (gdh) identified four novel multi-locus genotypes (MLGs), including two assemblage E MLGs and two assemblage A MLGs. These results suggested that Boar, Guanzhong dairy and Shanbei cashmere goats in Shaanxi province would be potential reservoirs for human infections in this area, and this study also provided basic data for controlling G. duodenalis infection in goats as well as other hosts., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
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15. Veterinary parasitology teaching in China in the 21st century - Challenges, opportunities and perspectives.
- Author
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Liu GH, Zhang LX, Zou FC, Yuan ZG, Zhao GH, Hu M, Suo X, and Zhu XQ
- Subjects
- Animals, China epidemiology, Genomics, History, 21st Century, Humans, Livestock, Parasitic Diseases, Animal parasitology, Parasitology history, Schools, Veterinary, Technology, Transcriptome, Zoonoses, Education, Veterinary, Parasitic Diseases, Animal epidemiology, Parasitology education, Students, Health Occupations, Teaching history
- Abstract
China has made significant achievements in social-economic development in the last three decades, and the numbers of livestock and companion animals are rapidly increasing. Some advances have been made in the control and prevention of animal parasitic diseases, but there are still some significant challenges, particularly in relation to foodborne parasitic zoonoses and vector-borne diseases. In addition, new molecular (e.g., genomic and transcriptomic) technologies have been developed and are gradually being introduced into the veterinary parasitology field. Therefore, teaching of veterinary parasitology in Chinese universities has undergone significant changes over the years, in terms of topics, depth and breadth, and also in the ways in which courses are delivered. In this article, we describe the current status of veterinary parasitology teaching at the undergraduate and postgraduate levels in Chinese universities, summarise changes and improvement in veterinary parasitology teaching, and discuss the challenges and opportunities for veterinary parasitology teaching in the 21st century, including the use of new teaching technologies and the integration of the "One Health" concept into veterinary parasitology courses., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
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16. Nuclear delivery of parasite Cdg2_FLc_0220 RNA transcript to epithelial cells during Cryptosporidium parvum infection modulates host gene transcription.
- Author
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Zhao GH, Gong AY, Wang Y, Zhang XT, Li M, Mathy NW, and Chen XM
- Subjects
- Animals, Cell Line, Tumor, Cryptosporidiosis parasitology, Cryptosporidium parvum physiology, Gene Expression Regulation, Humans, Positive Regulatory Domain I-Binding Factor 1 genetics, Transcriptome, Up-Regulation, Cryptosporidium parvum genetics, Host-Pathogen Interactions genetics, Intestinal Mucosa parasitology, RNA, Protozoan genetics, Transcription, Genetic
- Abstract
Intestinal infection by the zoonotic protozoan, Cryptosporidium parvum, causes significant alterations in the gene expression profile in host epithelial cells. The molecular mechanisms of how C. parvum may modulate host cell gene transcription and the pathological significance of such alterations are largely unclear. Previous studies demonstrate that a panel of parasite RNA transcripts are delivered into infected host cells and may modulate host gene transcription. Using in vitro models of intestinal cryptosporidiosis, in this study, we analyzed the impact of host delivery of C. parvum Cdg2_FLc_0220 RNA transcript on host gene expression profile. We found that alterations in host gene expression profile following C. parvum infection were partially associated with the nuclear delivery of Cdg2_FLc_0220. Specifically, we identified a total of 46 overlapping upregulated genes and 8 overlapping downregulated genes in infected cells and cells transfected with Full-Cdg2_FLc_0220. Trans-suppression of the DAZ interacting zinc finger protein 1 like (DZIP1L) gene, the top overlapping downregulated gene in host cells following C. parvum infection and cells transfected with Full-Cdg2_FLc_0220, was mediated by G9a, independent of PRDM1. Cdg2_FLc_0220-mediated trans-suppression of the DZIP1L gene was independent of H3K9 and H3K27 methylation. Data from this study provide additional evidence that delivery of C. parvum Cdg2_FLc_0220 RNA transcript in infected epithelial cells modulates the transcription of host genes, contributing to the alterations in the gene expression profile in host epithelial cells during C. parvum infection., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
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17. First genotyping of Blastocystis sp. in dairy, meat, and cashmere goats in northwestern China.
- Author
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Song JK, Yin YL, Yuan YJ, Tang H, Ren GJ, Zhang HJ, Li ZX, Zhang YM, and Zhao GH
- Subjects
- Age Factors, Animals, Blastocystis isolation & purification, China epidemiology, Dairy Products parasitology, Feces parasitology, Female, Genotype, Goats, Humans, Meat parasitology, Prevalence, Virulence, Blastocystis genetics, Blastocystis Infections genetics, Blastocystis Infections veterinary
- Abstract
Blastocystis is one of the most common parasites inhabiting in small intestines of human and animals. Although its pathogenicity has been remaining controversial, the possibility of zoonotic transmission between human and animals was recognized. The goat was one of the most important economic animals supplying people with cashmere, meat, and dairy products. However, few studies were to investigate Blastocystis infection in goats. A total of 789 faecal specimens of goats (including 362 of dairy, 193 of meat and 234 of cashmere goats) were collected from multiple regions of Shaanxi province in northwestern China to investigate the colonization frequency and subtypes of Blastocystis, and to assess the zoonotic potential of these goats. The respective colonization frequencies of Blastocystis in dairy, meat and cashmere goats were 54.1% (196/362), 40.4% (78/193) and 78.6% (184/234). The prevalence of Blastocystis in pre-weaned (0-2-month) goats was significantly lower than that in goats of other age groups, and the highest colonization was observed in goats of 7-11-month age group. Sequence analysis of Blastocystis positive samples indicated the presence of seven subtypes in these goats, including six known subtypes (STs1, 3, 4, 5, 10, 14) and one possible novel subtype (isolate Sd26), with the subtype 10 as the predominant one. Additionally, zoonotic subtypes were found in dairy (ST1, ST3 and ST5) and cashmere (ST4 and ST5) goats, but not detected in meat goats. These results showed that Blastocystis is highly prevalent, widely distributed and genetically diverse in goats in Shaanxi province, northwestern China, and zoonotic potential of dairy and cashmere goats to transmit Blastocystis., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
- Full Text
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18. Vaccination with a DNA vaccine encoding Toxoplasma gondii ROP54 induces protective immunity against toxoplasmosis in mice.
- Author
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Yang WB, Zhou DH, Zou Y, Chen K, Liu Q, Wang JL, Zhu XQ, and Zhao GH
- Subjects
- Animals, Antibodies, Protozoan blood, Female, Immunity, Cellular, Injections, Intramuscular, Interleukin-10 blood, Interleukin-12 blood, Interleukin-2 blood, Interleukin-4 blood, Mice, Plasmids, Recombinant Proteins immunology, Toxoplasma immunology, Antigens, Protozoan immunology, Cytokines blood, Immunologic Factors immunology, Protozoan Vaccines immunology, Toxoplasmosis immunology, Vaccination, Vaccines, DNA immunology
- Abstract
Toxoplasma gondii is an obligatory intracellular protozoan, which infects most of the warm-blooded animals, causing serious public health problems and enormous economic losses worldwide. The rhoptry effector protein 54 (ROP54) has been indicated as a virulence factor that promotes Toxoplasma infection by modulating GBP2 loading onto parasite-containing vacuoles, which can modulate some aspects of the host immune response. In order to evaluate the immuno-protective value of ROP54, we constructed a eukaryotic recombinant plasmid expressing T. gondii ROP54 and intramuscularly immunized Kunming mice with this recombinant plasmid against acute and chronic toxoplasmosis. All mice immunized with pVAX-ROP54 elicited a high level of specific antibody responses, a significant increase of lymphocyte proliferation, and a significant level of Th1-type cytokines (IFN-γ, IL-2 and IL-12p70), in addition to an increased production of Th2-type cytokines (IL-4 and IL-10). These results demonstrated that pVAX-ROP54 induced significant cellular and humoral (Th1/Th2) immune responses, which extended the survival time (13.0±1.15days for pVAX-ROP54 vs 6.7±0.48days for pVAX I, 6.8±0.42days for PBS and 6.5±0.53 for blank control) and significantly reduced cyst burden (35.9% for pVAX-ROP54, 1% for pVAX I and 2% for PBS, compared with blank control) of immunized mice. These results indicate that the recombinant ROP54 plasmid can provide partial protection and might be a potential vaccine candidate against acute and chronic toxoplasmosis., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
- Full Text
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19. Molecular characterization of Blastocystis from pigs in Shaanxi province of China.
- Author
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Song JK, Hu RS, Fan XC, Wang SS, Zhang HJ, and Zhao GH
- Subjects
- Animals, Blastocystis Infections epidemiology, China epidemiology, DNA, Ribosomal genetics, Feces parasitology, Female, Genetic Variation, Humans, Prevalence, Swine, Swine Diseases epidemiology, Blastocystis genetics, Blastocystis Infections veterinary, Swine Diseases parasitology
- Abstract
Blastocystis is an enteric eukaryote of mystery for its ubiquitous presence in animals and humans worldwide and a broad diversity genetically. The animals have been suggested to be an important reservoir to transmit Blastocystis to humans because of high colonization frequency and the presence of zoonotic subtypes. In the present study, the prevalence and subtypes of Blastocystis in pigs in Shaanxi province of China were determined using the molecular technique based on the small subunit rRNA (SSU rRNA) gene fragment. Of 560 pig faecal samples collected from different geographical origins, 419 (74.8%) were positive for Blastocystis colonization. The prevalence was significant affected by the age and the geographical origin. Four subtypes, including three zoonotic (ST1, ST3 and ST5) and one animal specific (ST10) subtypes, were identified. To our knowledge, this study provides the first run-through information for colonization of Blastocystis in pigs in China., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
20. Phylogenetic study of Baylisascaris schroederi isolated from Qinling subspecies of giant panda in China based on combined nuclear 5.8S and the second internal transcribed spacer (ITS-2) ribosomal DNA sequences.
- Author
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Zhao GH, Li HM, Ryan UM, Cong MM, Hu B, Gao M, Ren WX, Wang XY, Zhang SP, Lin Q, Zhu XQ, and Yu SK
- Subjects
- Animals, Bayes Theorem, China, DNA, Helminth genetics, Female, Genetic Markers, Genetic Variation, Male, Sequence Alignment, Sequence Analysis, DNA, Ascaridoidea genetics, Ascaridoidea isolation & purification, DNA, Ribosomal Spacer genetics, Phylogeny, Ursidae parasitology
- Abstract
The nuclear ribosomal DNA (rDNA) region spanning 5.8S rDNA and the second internal transcribed spacer (ITS-2) of Baylisascaris schroederi isolated from the Qinling subspecies of giant panda in Shaanxi Province, China were amplified and sequenced. Sequence variations in the two rDNA regions within B. schroederi and among species in the family Ascarididae were examined. The lengths of B. schroederi 5.8S and ITS-2 rDNA sequences were 156 bp and 327 bp, respectively, and no nucleotide variation was found in these two rDNA regions among the 20 B. schroederi samples examined, and these ITS-2 sequences were identical to that of B. schroederi isolated from giant panda in Sichuan province, China. The inter-species differences in 5.8S and ITS-2 rDNA sequences among members of the family Ascarididae were 0-1.3% and 0-17.7%, respectively. Phylogenetic relationships among species in the Ascarididae were re-constructed by Bayesian inference (Bayes), maximum parsimony (MP), and maximum likelihood (ML) analyses, based on combined sequences of 5.8S and ITS-2 rDNA. All B. schroederi samples clustered together and sistered to B. transfuga with high posterior probabilities/bootstrap values, which further confirmed that nematodes isolated from the Qinling subspecies of giant panda in Shaanxi Province, China represent B. schroederi. Because of the large number of ambiguously aligned sequence positions (difficulty of inferring homology by positions), ITS-2 sequence alone is likely unsuitable for phylogenetic analyses at the family level, but the combined 5.8S and ITS-2 rDNA sequences provide alternative genetic markers for the identification of B. schroederi and for phylogenetic analysis of parasites in the family Ascarididae., (Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2012
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21. Genomics and molecular genetics of Clonorchis sinensis: current status and perspectives.
- Author
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Huang SY, Zhao GH, Fu BQ, Xu MJ, Wang CR, Wu SM, Zou FC, and Zhu XQ
- Subjects
- Animals, Clonorchiasis epidemiology, Clonorchiasis parasitology, Clonorchiasis prevention & control, Expressed Sequence Tags, Fish Diseases diagnosis, Fish Diseases epidemiology, Fishes parasitology, Genetic Variation, Humans, MicroRNAs analysis, Nucleic Acid Amplification Techniques veterinary, Nucleic Acids analysis, Polymerase Chain Reaction veterinary, Snails parasitology, Transcriptome, Clonorchiasis diagnosis, Clonorchis sinensis genetics, Genome, Helminth, Molecular Sequence Annotation methods, Nucleic Acid Amplification Techniques methods, Polymerase Chain Reaction methods
- Abstract
Clonorchiasis caused by Clonorchis sinensis is an important foodborne parasitosis of humans and animals, and is predominantly a hepatobiliary disease. Globally, nearly 35 million people were infected with C. sinensis, with approximately 15 million being in China. Patients would chronically present fatigue, jaundice, abdominal discomfort, along with the increased risk of developing into a form of cholangiocarcinoma that is fatal to humans. Treatment of clonorchiasis by praziquantel has been very successful, but this is dependent on early accurate diagnosis and correct species identification. The present article reviews the current status of knowledge in genomics and functional genomics of C. sinensis, and summarizes the main DNA-based techniques for the specific diagnosis of C. sinensis infection and studies of genetic variation in C. sinensis, and provides perspectives for future studies. The advances in genomics and molecular genetics of C. sinensis shed new sight on our understanding of population structure of C. sinensis as well as the prevention and control of clonorchiasis., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2012
- Full Text
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22. Use of microorganisms immobilized on composite polyurethane foam to remove Cu(II) from aqueous solution.
- Author
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Zhou LC, Li YF, Bai X, and Zhao GH
- Subjects
- Adsorption, Cells, Immobilized, Copper isolation & purification, Hydrogen-Ion Concentration, Kinetics, Solutions, Temperature, Bacteria metabolism, Biodegradation, Environmental, Copper pharmacokinetics, Polyurethanes
- Abstract
Composite polyurethane (PU) foams were made via the polymerization of toluene diisocyanate (TDI) and polyether polyol with activated carbon fiber, and immobilized microorganisms on polyurethane (IPU) foam were prepared by cultivating the microbe B350 in a mixture of culture medium and PU. We used batch adsorption techniques to study the removal of Cu(II) ions from aqueous solutions via PU and IPU. Moreover, the effects of pH, temperature, carrier amount, and biosorption time on the removal rate of Cu(II), adsorption equilibrium, and adsorption kinetics were investigated in detail. The IPU showed an excellent removal rate for Cu(II). The adsorption kinetics data were in good agreement with the pseudo-second-order rate model, and the adsorption isotherms could be adequately described by the Langmuir equation. For synthetic wastewater containing Cu(II), the removal rates for Cu(II) and COD after 4h treatment were 85% and 80%, respectively.
- Published
- 2009
- Full Text
- View/download PDF
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