Search

Adipose tissue-derived stem cells (ADSCs) possess the capability to modulate the immune response and alleviate inflammation, rendering them a promising therapeutic option for various conditions, including autoimmune diseases, cardiovascular diseases, and tissue injuries. The osteogenic differentiation in ADSCs plays a pivotal role in fracture healing, bone growth, and the overall bone turnover process, governed by intricate interactions. Runt-related Transcription Factor 2 (RUNX2) is a key player in mineralized tissue generation and is typically found in the early stages of osteogenic differentiation. The objective of this study was to develop a high-affinity sandwich biosensor for the quantification of RUNX2. 1,1′-Carbonyldiimidazole-modified nanodiamond was immobilized on an amine-modified interdigitated electrode surface, followed by the use of a capture antibody to facilitate antigen interaction. A sandwich assay was conducted with the antibody, and the limit of detection for RUNX2 was calculated as 0.1 ng/mL, with a regression value (R2) of 0.9914 over a linear range of 1–2000 ng/mL. Furthermore, biofouling experiments with a nonimmune antibody, BSA, and TNF-α did not yield any current responses, indicating the specific detection of RUNX2. Additionally, RUNX2-spiked serum exhibited an increasing current response at all concentrations, confirming the selective detection of RUNX2.

This study investigates the mixed convection flow in a channel with a dimpled section and an adiabatic cylindrical obstacle under the influence of a magnetic field. The channel has a heated dimpled portion, while the flat horizontal walls are adiabatic. The energy equation considers the Joule heating effect, and the resulting momentum and thermal transport equations are numerically solved using the Galerkin weighted residual finite element method. The study discusses the flow and heat transfer characteristics for different values of the Joule heating parameter, Reynolds, Hartman, and Rayleigh numbers. The results show that increasing Hartmann and Joule heating parameters reduce average heat transfer rates while increasing Prandtl, Reynolds, and Rayleigh numbers enhances it. This analysis's outcomes will help design Ohmic heating systems like magnetic fluid power actuators and thermal processing gadgets for particulate food products. The novel quantitative findings in this study include enhancement in heat transfer with increasing Prandtl, Reynolds, and Rayleigh numbers. Furthermore, the adiabatic cylindrical obstacle near the dimpled section significantly affects the heat transfer rates, resulting in complex flow patterns. The results of this study can be used in magnetic fluid power actuators, thermal processing gadgets for particulate food products, and heat exchangers.

ABSTRACT: Objective: To differentiate diagnostic and prognostic factors from the clinical material of patients with cerebral sparganosis in central South China. Methods: Consecutive patients with cerebral sparganosis from our hospital between 2010 and 2018 were retrospectively enrolled. The clinical manifestations, radiographic features, treatment, and outcomes of these patients were analyzed. Results: Thirty patients with cerebral sparganosis were included, and foci migration on magnetic resonance imaging was detected in 22 patients, from whom we observed 4 migration modes: interlobar migration (50.0%, 11/22); transmidline migration (27.3%, 6/22); transventricular migration (13.6%, 3/22); and cerebellum-brainstem migration (9.1%, 2/22). The percentage of good outcomes was higher in patients with live worm capture than in those without live worm capture (75.0%, 12/16 vs 33.3%, 2/6). Exposure to preoperative antiparasitic medication was associated with worm migration toward the cortical surface, which led to a higher probability of live worm capture. Conclusions: We propose 4 modes of sparganosis migration that are correlated with worm capture and neurologic prognosis. We found that exposure to antiparasitic medication was associated with worm migration toward the cortical surface, leading to a higher probability of live worm capture. These observations suggest a novel significance for preoperative medication of cerebral sparganosis.