1. Elaboration and characterization of ε-polylysine‑sodium alginate nanoparticles for sustained antimicrobial activity.
- Author
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Zhou X, Guan C, Ma Q, Lan T, Lin Q, Zhou W, and Liu C
- Subjects
- Particle Size, Anti-Infective Agents pharmacology, Anti-Infective Agents chemistry, Staphylococcus aureus drug effects, Molecular Weight, Escherichia coli drug effects, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Microbial Sensitivity Tests, Drug Carriers chemistry, Alginates chemistry, Alginates pharmacology, Polylysine chemistry, Polylysine pharmacology, Nanoparticles chemistry
- Abstract
The ε-polylysine (ε-PL) is a food-grade antimicrobial substance. The cationic ε-PL molecules may interact with anionic components of food matrix causing turbidity, sedimentation, and hampering the antimicrobial activity. Herein, sodium alginate (SA) was used as wall material to encapsulate ε-PL, thereby to synthesize ε-PL-SA nanoparticles (ε-PL-SA-NPs). Monosaccharide composition and molecular weight of SA were characterized. The synthetic scheme is optimized and physicochemical characteristics and antimicrobial potential was investigated. Findings indicate that SA primarily consisted of mannuronic acid (95.25 %), weight average molecular weight (Mw) of SA was 176.464 kDa, and the molecular configuration of SA was irregular line clusters. The encapsulation efficiency (EE) of ε-PL in ε-PL-SA-NPs made under optimum strategy (at pH 6.0, mass ratio of ε-PL to SA is 0.14, and SA concentration is 6 mg/mL) is about 99.74 %. The particle size of ε-PL-SA-NPs is ∼541.86 nm. The SEM image showed that the ε-PL-SA-NPs had a nearly spherical morphology. Zeta-potential and FTIR data reveal the interaction between ε-PL and SA was electrostatic and the hydrogen bonding. Agar diffusion assay exhibit that ε-PL-SA-NPs had antimicrobial activity against Escherichia coli and Staphylococcus aureus. The salmon preservation experiments reveal sustained antimicrobial efficacy of ε-PL-SA-NPs., Competing Interests: Declaration of competing interest The authors declare no competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
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