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Nanobubbles (NBs) technology has been proven to promote methane production from anaerobic digestion (AD). In this study, the synergistic effects of (CH 4  + CO 2 )-nanobubble water ((CH 4  + CO 2 )-NBW) combined with varying particle sizes of corn straw on the AD were investigated. As findings, adding (CH 4  + CO 2 )-NBW effectively promoted the methane production from AD of corn straw with different particle sizes. The maximum cumulative methane yield (186.42 mL/ g-volatile solids) was achieved in Group a with the addition of (CH 4  + CO 2 )-NBW, representing a 16.89 % increase compared to the control. Furthermore, (CH 4  + CO 2 )-NBW could enhance the enzymatic activity. The activities of β-glucosidase and coenzyme F 420 were increased by 6.70 % and 11.48 %, respectively. The results of microbial community structure revealed that the addition of (CH 4  + CO 2 )-NBW could improve the abundance of dominant bacteria (norank_JS1, norank_Aminicenantales, and Bacteroidetes_vadinHA17) and archaea (Methanomassiliicoccaceae, Methanobacteriaceae, and norank_Bathyarchaeia). This study provides new insights into the application of nanobubbles in the AD of biomass., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Based on a novel bio-degumming system, the effect of chitosan on the degumming effect of ramie was investigated. The degumming effect indexes before and after the addition of chitosan were assessed, and the enzyme activities (pectinase, xylanase, ligninase and cellulase) were detected. Meanwhile changes in microbial community structure were evaluated. Furthermore, the electron-donating effect of chitosan and cellulose was further simulated by Fukui function. Degumming effect indexes showed the addition of chitosan could effectively increase the breaking strength of degummed ramie (from 6.04 to 6.59 cN/dtex), reduce the fineness (from 10.06 to 8.039 dtex), so that more gums were removed (the residual gum ratio reduced from 16.55 % to 12.09 %) and more cellulose was retained in the degummed ramie (cellulose content increased from 78.01 % to 80.96 %). Enzyme activity measurements revealed that the addition of chitosan increased the activity of degumming enzymes (pectinase, xylanase and ligninase), while decreased cellulase activity. The addition of chitosan induced changes of the community structure, with an increase of degumming microorganisms and a decrease of cellulose-degrading microorganisms. According to Fukui function, chitosan has a stronger electron donating ability than cellulose, which might be one important reason for the changes in enzyme activities and community structures., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Biofilms offer a solution to the challenge of low biomass retention faced in mainstream partial nitritation/Anammox (PN/A) applications. In this study, a one-stage PN/A reactor derived from initial granular sludge was successfully transformed into a biofilm system using shedding carriers. Environmental stressors, such as ammonium nitrogen concentration and organic matter, significantly affected the competitive dynamics and dominant species composition between Ca. Kuenenia and Ca. Brocadia. Under approximately 500 mg/L NH 4 + -N, Ca. Brocadia emerged as the dominant anammox bacteria species, but was subsequently replaced by Ca. Kuenenia in the presence of approximately 54 mg COD/L CH 3 COONa. Moreover, Chloroflexi species on the original biofilm exhibited an associated relationship with the growth of Ca. Kuenenia in new biofilm. The biofilm assembly and microbial community migration uniquely reveal the microbial niche dynamics. This study provides valuable insights for PN/A biofilm applications facing diverse challenges of environmental stresses in the transition from sidestream to mainstream., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

This study evaluated the effectiveness of embedding immobilization technology in wastewater treatment and its capacity to enhance the hydrolysis acidification process. Based on this technology, a stable anaerobic environment has been maintained. Results showed that the rates of dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP) conversion both exceeded 98 % under short hydraulic retention time (HRT = 2h) and ambient temperature. Notably, acetic acid and propionic acid comprised up to 90.9 % of the total volatile fatty acids in the effluent, providing suitable carbon sources for downstream denitrification. 16S rRNA gene sequencing indicated that biofillers effectively enriched and retained functional bacteria, causing norank_Anaerolineaceae (11.6 %-29.7 %) and norank_Bacteroidetes_vadinHA17 (10.8 %-14.9 %) as the dominant genera in the reactor, which were crucial for refractory organic matter degradation. Immobilized biofillers effectively improved wastewater biodegradability, supporting a stable microbial community with high DON and DOP conversion rates as well as increased VFA accumulation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

The anammox process is efficient for nitrogen removal but faces challenges due to slow bacterial growth and limited inoculated sludge supply. This study examined the effects of different inoculated sludge concentrations (3.5, 7, and 14 g/L) on start-up and nitrogen metabolism in anammox reactors. Three identical reactors were operated under controlled conditions, with comprehensive analysis of nitrogen removal efficiency, sludge characteristics, and microbial community dynamics through metagenomic and transcriptomic approaches. Results demonstrated that higher inoculated sludge concentrations accelerated reactor start-up, with the 14 g/L reactor achieving stable operation in 13 days compared to 44 days for the 3.5 g/L reactor. However, the improvement in nitrogen removal rate showed a boundary effect, not proportional to the increase in sludge concentration. Notably, reactors with higher inoculated sludge concentrations exhibited lower sludge loads but higher sludge yield coefficients. Metagenomic analysis revealed Candidatus Kuenenia as the dominant anammox bacteria, with decreasing hydrazine dehydrogenase (hdh) gene expression levels observed at higher sludge concentrations, suggesting hydrazine synthesis as a potential rate-limiting step. This study provides novel insights into the optimal range of inoculated sludge concentration for anammox reactor start-up and elucidates the underlying metabolic mechanisms, offering valuable guidance for practical engineering applications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Developing methods for reusing biodegradable plastics, like polylactic acid (PLA) straws, is highly needed. Here, PLAs were applied to substitute traditional commercial ceramic media (CCM) in denitrification biofilters. During long-term operation, replacing CCM with PLA significantly enhanced nitrate removal efficiency from 32.68-54.39 % to 41.64-66.26 %. Ammonia nitrogen effluent maintained below 0.5 mg/L in all reactors. PLA plastisphere shaped unique microbial communities, i.e., denitrifying bacteria Bacillus, Pseudomonas and Acidovorax preferred to inhabit or degrade PLA. Compared to CCM biofilms, PLA diminished the importance of stochastic process in biofilm assembly of PLA plastisphere. Metagenomic sequencing suggested that PLA biofilms possessed greater metabolic capabilities of denitrification and glycolysis compared to CCM. Additionally, Bacillus strain P01 isolated from PLA plastisphere demonstrated strong PLA depolymerization. Overall, this study revealed that PLA serves as carbon source and biofilm carrier, offering a promising approach to integrating plastic reuse with wastewater treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Tunnel Oxide Passivating Contacts (TOPcon) battery in the photovoltaic industry generates high ammonium wastewater during the production process, the adaptability of using the anaerobic ammonia oxidation (Anammox) process for photovoltaic wastewater (PVW) treatment is a research hotspot. Based on the analysis of photovoltaic wastewater quality, the effectiveness of nitrogen removal, sludge characteristics and microbial communities were examined. The results showed that when the influent NH 4 + -N concentration of PVW was lower than 150 mg·L -1 , the nitrogen removal efficiency (NRE) was almost 100 %. In addition, the NRE decreased from 74 % sharply to 20 % when the NH 4 + -N concentration was increased from 175 mg·L -1 to 200 mg·L -1 . The extracellular polymeric substances (EPS) content increased with elevated ammonium concentration in the influent, indicating that microorganisms secreted more EPS to resist elevated nitrogen loading. The main functional populations were Candidatus Kuenenia (0-24 %). The influent ammonium concentration is recommended to be < 200 mg·L -1 ., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Triclosan (TCS), a hydrophobic antibacterial agent, is extensive application in daily life. Despite a low biodegradability rate, its hydrophobicity results in its accumulation in waste-activated sludge (WAS) during domestic and industrial wastewater treatment. While anaerobic digestion is the foremost strategy for WAS treatment, limited research has explored the interphase migration behavior and impacts of TCS on WAS degradation during anaerobic digestion. This study revealed TCS migration between solid- and liquid-phase in WAS digestion. The solid-liquid distribution coefficients of TCS were negative for proteins and polysaccharides and positive for ammonium. High TCS levels promoted volatile-fatty-acid accumulation and reduced methane production. Enzyme activity tests and functional prediction indicated that TCS increased enzyme activity associated with acid production, in contrast to the inhibition of key methanogenic enzymes. The findings of the TCS migration behavior and its impacts on WAS anaerobic digestion provide an in-depth understanding of the evolution of enhanced TCS-removing technology., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Food waste (FW) and its biogas residue were considered as sources of terrestrial microplastics (MPs) and phthalic acid esters (PAEs) contamination. However, there was a lack of research and understanding of the MPs and PAEs pollution problem in FW dry anaerobic digestion process (DADP). The MPs and PAEs in three stages of the DADP with the largest monomer disposal scale in China were identified. At the biogas residue extrusion stage, MPs abundance and PAEs concentration reached the highest values, which were 3.63 ± 0.45 × 10 3 N·kg -1 and 3.62 ± 0.72 mg·kg -1 , respectively. Furthermore, there was a significant positive correlation between MPs and PAEs throughout the process (p < 0.05). Although bacteria and fungi with plastic degradation potential were present in all stages, the contamination problem of MPs and PAEs cannot be completely solved through DADP. This study provides a scientific basis for preventing and controlling the pollution of MPs and PAEs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

A bioelectrochemical upflow anaerobic sludge blanket (BE-UASB) was constructed and compared with the traditional UASB to investigate the role of bioelectrocatalysis in modulating methanogenesis and sulfidogensis involved within anaerobic treatment of high-sulfate methanolic wastewater (COD/SO 4 2- ratio ≤ 2). Methane production rate for BE-UASB was 1.4 times higher than that of the single UASB, while SO 4 2- removal stabilized at 16.7%. Bioelectrocatalysis selectively enriched key functional anaerobes and stimulated the secretion of extracellular polymeric substances, especially humic acids favoring electron transfer, thereby accelerating the electroactive biofilms development of electrodes. Methanomethylovorans was the dominant genus (35%) to directly convert methanol to CH 4 . Methanobacterium as CO 2 electroreduction methane-producing archaea appeared only on electrodes. Acetobacterium exhibited anode-dependence, which provided acetate for sulfate-reducing bacteria (norank Syntrophobacteraceae and Desulfomicrobium) through synergistic coexistence. This study confirmed that BE-UASB regulated the microbial ecology to achieve efficient removal and energy recovery of high-sulfate methanolic wastewater., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Acid accumulation and carbon emission are two major challenges in anaerobic digestion. Syntrophic consortia can employ reverse electron transfer (RET) to facilitate thermodynamically unfavorable redox reactions during acetogenesis. However, the potential mechanisms and regulatory methods of RET remain unclear. This study examines the regulatory mechanisms by which exogenous CO 2 affects RET and demonstrates that biochar maximizes CO 2 solubility at 25.8 mmol/L to enhance effects further. CO 2 synergized with biochar significantly increases cumulative methane production and propionate degradation rate. From the bioenergetic perspective, CO 2 decreases energy level to a maximum of -87 kJ/mol, strengthening the thermodynamic viability. The underlying mechanism can be attributed to RET promotion, as indicated by increased formate dehydrogenase and enrichment of H 2 /formate-producing bacteria with their partner Methanospirillum hungatei. Moreover, the 5 % 13 CH 4 and methane contribution result show that CO 2 accomplishes directed methanogenesis. Overall, this investigation riches the roles of CO 2 and biochar in AD surrounding RET., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The productivity and efficiency of two-chamber microbial electrolysis cell and anaerobic digestion integrated system (MEC-AD) were promoted by a complex of anaerobic granular sludge and iron oxides (Fe-AnGS) as inoculum. Results showed that MEC-AD with Fe-AnGS achieved biogas upgrading with a 23%-29% increase in the energy recovery rate of external circuit current and a 26%-31% decrease in volatile fatty acids. The energy recovery rate of MEC-AD remained at 52%-57%, indicating a stable operation performance. The selectively enriched methanogens and electroactive bacteria resulted in dominant hydrogenotrophic and acetoclastic methanogenesis in the cathode and anode chambers. Mechanistic analysis revealed that MEC-AD with Fe-AnGS led to specifically upregulated enzymes related to energy metabolism and electron transfer. Fe-AnGS as inoculum could improve the long-term operation performance of MEC-AD. Consequently, this study provides an efficient strategy for biogas upgrading in MEC-AD., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Dark fermentation (DF) is an eco-friendly process that simultaneously achieves organic matter degradation and obtains hydrogen (H 2 ). Nonetheless, low H 2 yield mainly caused by poor activity of key microbes, is still a problem that requires being resolved. In this work, MgFe 2 O 4 and Ca 0.5 Mg 0.5 Fe 2 O 4 nanoparticles (NPs) were synthetized and served as additives to boost H 2 form from DF. H 2 productivity gradually increased with the rise of NPs, and declined when NPs exceeded their optimal dosages. The highest H 2 yield was 183.6 ± 3.2 mL/g glucose at 100 mg/L of MgFe 2 O 4 NPs, being 35.2 % higher than that of the control yield (135.8 ± 3.1 mL/g glucose). However, the highest H 2 yield of 171.9 ± 2.5 mL/g glucose occurred at 400 mg/L of Ca 0.5 Mg 0.5 Fe 2 O 4 NPs, increasing by 26.6 % over the control. Interestingly, the two NPs favored the butyric acid pathway for H 2 synthesis. This provides guidance for multi-element oxide NPs used in DF., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Long-term high efficiency and stable partial nitrification (PN) performance was achieved using gel-immobilized partial nitrifying bacteria. The PN characteristics of the filler under high and low ammonia nitrogen concentrations and low temperature were comprehensively studied and the rapid reactivation was achieved after reactor breakdown or long stagnation period. The results showed that the maximum ammonia oxidation rate was 66.8 mg•(L•h) -1 and the nitrite accumulation rate was above 95 % for the filler. Efficient and stable PN performance depends on the high abundance of ammonia-oxidizing bacteria (AOB) inside the filler and dynamically microbial community. In addition, the oxygen-limited zone and competition between the microorganisms inside the filler effectively inhibited the growth of nitrite oxidizing bacteria, and the sludge outside the filler assisted in this process, which supported the dominant position of AOB in fillers. This study provides a reliable technology for the practical application of the PN nitrogen removal process., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Per- and polyfluoroalkyl substances (PFASs) are recalcitrant organic pollutants, which accumulate widely in aquatic and solid matrices. Anaerobic digestion (AD) is one of possible options to manage organic wastes containing PFASs, however, the impacts of different types of PFAS on AD remains unclear. This study aimed to critically investigate the effects of two representative PFAS compounds, i.e., perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS), on the AD performance and microbial community structure. 100 mg/L of both PFOA and PFOS considerably inhibited the AD performance and changed the microbial community structure. Especially, PFOA was more toxic to bacterial and archaeal activity than PFOS, which was reflected in AD performance. In addition, the sulfonic acid group in PFOS affected the changes in microbial community structure by inducing abundant sulfate reducing bacteria (i.e., Desulfobacterota). This study provides a significant reference to the response of AD system on different PFAS types and dosage., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

This work aimed to elucidate the metabolic mechanism of heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria influenced by varying concentrations of ammonium nitrogen (NH 4 + -N) in high-strength synthetic wastewater treatment. The results showed that the removal rates of NH 4 + -N and total nitrogen, along with enzymatic activities related to nitrification and denitrification, increased with rising NH 4 + -N concentrations (N 500 :500 mg/L, N 1000 :1000 mg/L and N 2000 :2000 mg/L). The relative abundances of HN-AD bacteria were 50 %, 62 % and 82 % in the three groups. In the N 2000 group, the cAMP signaling pathway, glycerophospholipid metabolites, purines and pyrimidines related to DNA/RNA synthesis, electron donor NAD + -related energy, the tricarboxylic acid (TCA) cycle and glutamate metabolism were upregulated. Therefore, influent NH 4 + -N at 2000 mg/L promoted glutamate metabolism to accelerate the TCA cycle, and enhanced cellular energy and advanced denitrification activity of bacteria for HN-AD. This mechanism, in turn, enhanced microbial growth and the carbon and nitrogen metabolism of bacteria for HN-AD., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Pengying Xiao reports writing assistance was provided by American Journal Experts. Pengyinng Xiao reports a relationship with National Natural Science Foundation of China, Natural Science Foundation Project of Chongqing CSTC, Project of Science and Technology Research Program of Chongqing Education Commission of China that includes: funding grants. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The high levels of free ammonia (FA) challenge the application of partial nitritation (PN) and denitrification (DN) in the treatment of ammonia-rich wastewater. This study explored the impact of high levels of FA on the PN and DN stability and microbial community dynamics. By reducing reflux and increasing influent load, the concentrations of FA in PN and DN reactors increased from 28.9 mg/L and 140.0 mg/L to 1099.8 mg/L and 868.4 mg/L, respectively. During this process, the performance of PN and DN remained stable. The microbial analysis revealed that the Nitrosomonas exhibited strong tolerance to high levels of FA, and its relative abundance was positively correlated with amoABC (R 2 0.984) and hao (R 2 0.999) genes. The increase in microbial diversity could enhance the resistance ability of PN against the FA impact. In contrast, high levels of FA had scant influence on the microbial community and performance of DN., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

Aerobic denitrification technology can effectively abate the nitrogen pollution of water source reservoirs. In this study, 40% siliceous material was used as the carrier to replace the activated carbon in Fe/C material to enhance denitrification and purify water. The removal efficiency of new material for target pollutants were nitrate nitrogen (95.68%), total phosphorus (68.23%) and chemical oxygen demand (46.20%). Aerobic denitrification of water samples and anaerobic denitrification of sediments in three systems jointly assisted nitrogen removal. In a reactor with new material, diversity and richness of denitrifying bacterial communities were enhanced, and the symbiotic structure of aerobic denitrifying bacteria was more complex (Bacillus and Mycobacteria as the dominant bacteria); the microbial distribution better matched the Zif and Mandelbrot models. This system significantly increased the abundance of key enzymes in water samples. The new material effectively removed pollutants and represents a promising and innovative in-situ remediation method for reservoirs., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Generally, dark fermentation (DF) of hydrogen (H 2 ) synthesis has low H 2 production from industrial-scale plants. In this study, campus greening wastes-ginkgo leaves were used to produce molten salt-modified biochar (MSBC) and nitrogen (N 2 )-atmosphere BC (NBC) in molten salt and N 2 environment at 800 °C, respectively. MSBC showed excellent properties including high specific surface area and electron transfer ability. After supplementation with MSBC, H 2 yield rose by 32.4% compared to the control group without carbon material. Electrochemical analysis revealed MSBC improved the electrochemical properties of sludge. Furthermore, MSBC optimized the microbial community structure and increased the relative abundance of dominant microbes, thus promoting H 2 production. This work provides a deep understanding of two carbons that play vital roles in increasing microbial biomass, supplementing trace element and favoring electron transfer in DF reactions. Salt recovery achieved 93.57% in molten salt carbonization, which has sustainability compared with N 2 -atmosphere pyrolysis., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Aerobic granular sludge (AGS) has proved to be a promising biotechnology for microplastics wastewater treatment. However, polyacrylonitrile microplastics (PAN MPs), the most widely used plastic in textile materials, have not been investigated. Therefore, the effect of the neglected PAN MPs on AGS at different concentrations (1, 10, and 100 mg/L) was evaluated. The results indicated that PAN MPs with 1 and 10 mg/L concentrations had no obvious effect on granular stability and nutrient removal performance, but greatly promoted the secretion of EPS. Remarkably, the granule structure was severely damaged under 100 mg/L PAN MPs. Moreover, microbial community analysis showed that phylum Proteobacteria played a dominant role in resistance to PAN MPs. Metabolic analysis further revealed that genes related to denitrification pathway (nasA, nirK, nirS and norB) and membrane transport were significantly inhibited under PAN MPs stress. This study may provide additional information on the treatment of microplastics wastewater using AGS., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

The study aim was to reveal the mechanism of impact of two type biochar on composting of hen manure (HM) and wheat straw (WS). Biochar derived from coconut shell and bamboo used as additives to reduce antibiotic resistant bacteria (ARB) in HM compost. The results manifested that effect of biochar amendment was significant to reduce ARB in HM composting. Compared with control, the microbial activity and abundance were increased in both biochar applied treatment, and bacterial community was changed. Additionally, network analysis revealed that biochar amendment increased the quantity of microorganisms related to organic matter degrading. Among them, coconut shell biochar (CSB) played a pioneering role to mitigate ARB to better exert its effects. Structural correlation analysis showed that CSB reduce ARB mobility and promote organic matter degradation via improving beneficial bacterial community structure. Overall, composting with participation of biochar amendment stimulated antibiotic resistance bacterial dynamics. These results evidence practical value for scientific research and lay the foundation for agricultural promotion of composting., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The slow growth and difficulty in cultivating anammox bacteria limit the rapid start-up of anammox process and effective microbial enrichment. In this study, microbial electrolysis cell (MEC) was coupled with anammox to investigate the effects of different applying voltage methods on substrate removal efficiency and rates, microbial community structure, anammox metabolism and metabolic pathways. The results showed that applying voltage not only improved NH 4 + -N removal efficiency and removal rates, but also promoted electron transfer efficiency, key enzyme activity and extracellular polymeric substances (EPS) secretion in the systems. Step-up voltage was more conducive to the growth of Candidatus&#95;Kuenenia in the cathode, which promoted the rapid start-up of anammox and treating wastewater with low ammonia concentration. The main metabolic pathway in step-up voltage operation was hydrazine to nitrogen, while in constant voltage operation was hydroxylamine oxidation pathway. These findings provided a new insight into the enhancement and operation of anammox system., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: [Xiuhong Liu reports financial support was provided by R&D Program of Beijing Municipal Education Commission.]., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

This study established a stable and efficient pilot-scale denitrification (DN) and partial nitritation (PN) combined with autotrophic nitrogen removal process for mature landfill leachate treatment. A total inorganic nitrogen removal efficiency (TINRE) of 95.3% was achieved without any external carbon source input, including 17.1%, 1.0% and 77.2% of nitrogen removal contributed by the DN, PN and autotrophic processes, respectively. ANAMMOX genus, Ca&#95;Anammoxoglobus (19.4%) was dominant in autotrophic reactor. Moreover, denitrifying bacteria could utilize in-situ organics, including poorly degradable organics, to enhance the nitrogen removal performance of autotrophic process, contributing 3.4% of TINRE. This study provides new insights for the economical, low-carbon, and efficient treatment of mature landfill leachate., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

Marine anammox bacteria (MAB) have promising nitrogen removal performance in high saline wastewater treatment. Nevertheless, the impact resulting from moderate and low salinities on MAB is still unclear. Herein, MAB were applied to treat saline wastewater from high to moderate and low salinities for the first time. Independent of salinities (35-3.5 g/L), MAB consistently exhibited good nitrogen removal performance, and maximum total nitrogen removal rate (0.97 kg/(m 3 ·d)) occurred at 10.5 g/L salts. More extracellular polymeric substances (EPSs) were secreted by MAB-based consortia to resist hypotonic surroundings. However, a sharp EPS decrease was accompanied by the collapse of MAB-driven anammox process, and MAB granules disintegrated due to long-term exposure to salt-free environment. The relative abundance of MAB varied from 10.7% to 15.9% and 3.8% as salinity decreased from 35 to 10.5 and 0 g/L salts. These findings will provide practical implementation of MAB-driven anammox process treating wastewater with different salinities., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

In this work, the bioelectrochemical system (BES) is a feasible alternative for successfully degrading typical refractory emerging contaminant triclosan (TCS). A single-chamber BES reactor with an initial TCS concentration of 1 mg/L, an applied voltage of 0.8 V, and a solution buffered with 50 mM PBS degraded 81.4 ± 0.2% of TCS, exhibiting TCS degradation efficiency improvement to 90.6 ± 0.2% with a biocathode formed from a reversed bioanode. Both bioanode and biocathode were able to degrade TCS with comparable efficiencies of 80.8 ± 4.9% and 87.3 ± 0.4%, respectively. Dechlorination and hydrolysis were proposed as the TCS degradation pathway in the cathode chamber, and another hydroxylation pathway was exclusive in the anode chamber. Microbial community structure analysis indicated Propionibacteriaceae was the predominant member in all electrode biofilms, and the exoelectrogen Geobacter was enriched in anode biofilms. This study comprehensively revealed the feasibility of operating BES technology for TCS degradation., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

This study performed co-digestion of poultry litter (PL) with wheat straw in the daily anaerobic sequencing batch reactor considering operation parameters of carbon-to-nitrogen ratio (C/N, 11.6 to 28.4), total solids (TS, 2.6 to 9.4%), and hydraulic retention time (HRT, 7.6 to 24.4d). The inoculum with a diverse microbial community structure included 2% of methanogens (Methanosaeta) was chosen. Experimental performance by central composite design showed continuous methane production with the highest biogas production rate (BPR) obtained at C/N = 20, TS = 6%, and HRT = 7.6d, being (1.18 ± 0.14 L/L R /d). A significant modified quadratic model (p < 0.0001) for predicting BPR was built (R 2  = 0.9724). The operation parameters and process stability both affected the release of nitrogen, phosphorus, and magnesium in the effluent. The results provided new support for the novel reactor operations for efficient bioenergy production from PL and agricultural wastes., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Cu is widely present in the feedstocks of dark fermentation, which can inhibit H 2 production efficiency of the process. However, current understanding on the inhibitory mechanisms of Cu, especially the microbiological mechanism, is still lacking. This study investigated the inhibitory mechanisms of Cu 2+ on fermentative hydrogen production by metagenomics sequencing. Results showed that the exposure to Cu 2+ reduced the abundances of high-yielding hydrogen-producing genera (e.g. Clostridium sensu stricto), and remarkably down-regulated the genes involved in substrate membrane transport (e.g., gtsA, gtsB and gtsC), glycolysis (e.g. PK, ppgK and pgi-pmi), and hydrogen formation (e.g. pflA, fdoG, por and E1.12.7.2), leading to significant inhibition on the process performances. The H 2 yield was reduced from 1.49 mol H 2 /mol-glucose to 0.59 and 0.05 mol H 2 /mol-glucose upon exposure to 500 and 1000 mg/L of Cu 2+ , respectively. High concentrations of Cu 2+ also reduced the rate of H 2 production and prolonged the H 2 -producing lag phase., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

An engineering-scale sulfur driven autotrophic denitrification vertical-flow constructed wetland (SADN-VFCW) was established to treat low C/N ratio tailwater from municipal wastewater treatment plants (MWTPs). One-year stable operation results indicated that the addition of sulfur prominently enhanced TN, NO 3 - -N and TP removal with efficiencies higher than 68.9%, 69.2% and 45.5%, respectively. Higher nitrogen and phosphorus removal rates were achieved in summer than that in other seasons. Furthermore, the microbial analysis revealed the structure of the microbial community changed significantly after sulfur addition, which proved that sulfur promoted the enrichment of autotrophic (Thiobacillus, Sulfurimonas, Ferritrophicum) and heterotrophic (Denitratisoma, Anaerolineaae, Simplicispira) functional bacteria, thus facilitating pollutants removal. Function prediction analysis results also indicated the abundance of nitrate removal/sulfur metabolism functions was significantly strengthened. This study achieved reliable engineering-scale application of SADN-VFCW and offered great potential for simultaneous in-depth treatment of N and P in municipal tailwater by SADN system., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Azo dyes are significant organic pollutants known for their adverse effects on humans and aquatic life. In this study, anthraquinone-2-sulfonate (AQS) immobilized on biochar (BC) was employed as a novel carrier in up-flow anaerobic fixed-bed reactors to induce specific biofilm formation and promote the biotransformation efficiency of azo dyes. Novel carrier-packed reactor 1 (R1) and BC-packed reactor 2 (R2) were used to treat red reactive 2 (RR2) under continuous operation for 175 days. The decolorization rates of R1 and R2 were 96-83% and 91-73%, respectively. The physicochemical characteristics and extracellular polymeric substances (EPS) of the biofilm revealed a more stable structure in R1. Furthermore, the microbial community in R1 interacted more closely with each other and contained more keystone genera. Overall, this study provides a feasible method for improving the biotransformation of azo dyes, thus providing support for practical applications in wastewater treatment projects., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The accumulation of emerging organic contaminants (EOCs) in waste activated sludge (WAS) is a global concern. In this study, a multi-heteroatom nitrogen and sulfur was successfully embedded into lignin-based biochar (N-S-LGBC) and used it to activate calcium peroxide (CP) for the degradation of 4-nonylphenol (4-NP) in WAS. N-S-LGBC/CP was effective in degrading 85 % of 4-NP within 12 h through the activation of CP owing to hydroxyl radicals and singlet oxygen species generated from the synergism among pyrrolic-N, thiophenic-S, and lattice oxygen, i.e., active sites responsible for 4-NP degradation. These results highlight substrate biodegradability for subsequent bioprocesses that improves WAS treatment in EOC degradation by the N-S-LGBC/CP-mediated process. There was abundance of distinct Aggregatilinea genus within the phylum Chloroflexi during N-S-LGBC/CP treatment, indicating high 4-NP pretreatment efficiency in WAS. This work provides a new understanding of N-S-co-doped carbocatalysts in green and sustainable hydroxyl radical-driven carbon advanced oxidation (HR-CAOP) platforms for WAS remediation., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Carbon and nitrogen evolution and bacteria and fungi succession in two functional membrane-covered aerobic composting (FMCAC) systems and a conventional aerobic composting system were investigated. The micro-positive pressure in each FMCAC system altered the composting microenvironment, significantly increased the oxygen uptake rates of microbes (p < 0.05), and increased the abundance of cellulose- and hemicellulose-degrading microorganisms. Bacteria and fungi together influenced the conversion between carbon and nitrogen forms. FMCAC made the systems less anaerobic and decreased CH 4 production and emissions by 22.16 %-23.37 % and N 2 O production and emissions by 41.34 %-45.37 % but increased organic matter degradation and NH 3 production and emissions by 16.91 %-90.13 %. FMCAC decreased carbon losses, nitrogen losses, and the global warming potential by 7.97 %-11.24 %, 15.43 %-34.00 %, and 39.45 %-42.16 %, respectively. The functional membrane properties (pore size distribution and air permeability) affected fermentation process and gaseous emissions. A comprehensive assessment indicated that FMCAC has excellent prospects for application., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

A two-stage partial nitritation/anammox process based on microbial encapsulation (PN/A-E) was established. The nitrogen removal characteristics of PN/A-E under high and low ammonia nitrogen and after reactivation following a long-term shutdown were comprehensively investigated and compared with anammox granular sludge (AnGS). The stable PN process did not depend on high ammonia nitrogen, and the nitrite accumulation rate reached 95.2 ± 0.7 %. The overall nitrogen removal rate of encapsulated anammox bacteria was twice that of the AnGS, and it was more tolerant to external interference. Moreover, PN/A-E showed good reactivation performance, and the total nitrogen in the effluent was 10.0 ± 1.4 mg·L -1 when the final hydraulic retention time was 2.18 h. The immobilized fillers support an increase in ammonia-oxidizing bacteria under restricted conditions and were more conducive to the dominance of functional bacteria and the stability of microbial community under low ammonia nitrogen. This study provides a positive method to achieve a reliable PN/A., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Acid-pretreated pistachio shells were used as carbon sources to investigate the effects of carbon source dosage on simultaneous nitrogen and phosphorus removal under different carbon/nitrogen (C/N) ratios (7, 9, and 11). Results showed that C/N was positively correlated with mixed liquor suspended solids (MLSS) (R 2  = 0.998, p < 0.01) and f value (R 2  = 0.975, p < 0.05). Moreover, it was negatively correlated with the sludge volume index (SVI) (R 2  =  - 0.959, p < 0.05). C/N was also significantly negatively related to chemical oxygen demand removal rate (R 2  =  - 0.986, p < 0.05) and positively related to ammonia nitrogen (NH 4 + -N), total nitrogen (TN), and total phosphorus (TP) removal rate (p < 0.05), the correlation coefficients were 0.992, 0.990 and 0.994, respectively. In the reactor with C/N of 11, the MLSS concentration and f value were the highest, the SVI was the lowest, and the removal efficiencies of NH 4 + -N (85.49 % ± 1.96 %), TN (84.19 % ± 1.42 %) and TP (94.10 % ± 1.67 %) were the highest. Furthermore, the relative abundance of denitrifying bacteria was the highest in the reactor. The abundance of nitrifying bacteria and phosphorus-removal bacteria was also relatively high., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Co-composting of forest residues (FR) with cattle manure (CM) results in a low nitrogen (N) conversion efficiency, a low organic matter (OM) degradation rate, and a low quality compost product. This study evaluated the effects of addition of bone charcoal (BC), pumice (PM), or straw biochar (SB) at a ratio of 10 % (w/w) on the co-composting of FR with CM. The highest quality compost was obtained with addition of 10 % PM. Compared with the control (without any additive), PM addition increased the OM degradation rate, the nitrate-N, the available phosphorus, and the available potassium by 25 %, 110 %, 24 %, and 9 %, respectively, and increased the relative abundance of bacteria (Planomicrobium, Flavobacterium, and Pseudomonas) involved in lignocellulose degradation and N transformation. With the addition of PM, the co-composting of FR with CM generated a high quality, useful product in only 39 days., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

To address the problem of antibiotic mycelial dreg (AMD) treatment and removal of antibiotic resistance genes (ARGs), this study adopted anaerobic digestion (AD) technology, and added biochar (BC) and biochar loaded with nanosized zero-valent iron (nZVI-BC) to promote the AD of AMD and enhance the removal of ARGs. Results showed that nZVI-BC was better than BC in promoting AD due to the hydrogen evolution corrosion and the synergistic effect of nZVI and BC. In addition, BC and nZVI-BC can enhance the oxidative stress response and reduce ammonia stress phenomenon, which significantly reduces the abundance of aadA, ant(2″)-Ⅰ, qacEdelta1 and sul1. In conclusion, the enhance effect of nZVI-BC is greater than BC. The removal efficiency rates of nZVI-BC on the above-mentioned four ARGs were improved by 33%, 9%, 24% and 11%., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

To reveal the mechanisms of enhanced biological nitrogen and phosphorus removal by denitrifying phosphorus removal in a Micro-pressure swirl reactor (MPSR), this study used a MPSR to treat municipal wastewater and enriched denitrifying phosphate accumulating organisms (DPAOs) by using its alternating anaerobic-anoxic-aerobic environment. The coupling of denitrification phosphorus removal (DPR) and simultaneous nitrification endogenous denitrification phosphorus removal (SNEDPR) was achieved in MPSR, and the average removal rates of COD, NH 4 + -N, TN and TP were 91.57%, 98.51%, 85.88%, 96.08% respectively. The results of the batch experiments showed that DPAOs activity in the low dissolved oxygen (DO) and high DO zones were 70.5% and 74.3%. The results of intracellular carbon source conversion patterns, microbial assays and functional gene prediction indicated that Flavobacterium and Dechloromonas dominated the DPR process in the low DO zone. Based on these findings, nutrient removal pathways within the MPSR were integrated., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The management of potato peel waste (PPW) has been a challenge faced by the potato industry. This investigation assessed the feasibility of PPW for volatile fatty acids (VFAs) production via anaerobic fermentation, and investigated the impact of Fe 0 nanoparticles (Fe 0 NPs) supplementation on the VFAs production. It is found that PPW is a potential feedstock for producing VFAs, achieving a yield of 480.4 mg COD/g-vS Meanwhile, the supplementation of Fe 0 NPs significantly promoted the VFAs productivity and quality. The higher enrichment of VFAs-producing bacteria, including Clostridium, Proteiniphilum, Fonticella and Pygmaiobacter, contributed to the promotion of the VFAs yield. Furthermore, metagenomic analysis revealed that the encoding genes responsible for carbohydrate metabolism (especially starch), membrane transport, glycolysis and the formation of acetic and butyric acids were remarkably up-regulated,which could be the essential reason for the enhanced metabolic activity and VFAs productivity. This work provides a promising strategy for recycling PPW., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

In this work, metal-organic frameworks (MOFs) were prepared to evaluate its impact on carbon dioxide (CO 2 ) biomethanization during anaerobic degradation (AD). The results showed that MOFs significantly improved the CO 2 biomethanation efficiency, especially in the AD reactors using a concentration of 1.0 g/L MOFs. Furthermore, MOFs promoted direct interspecific electron transfer and alleviated the hydrogen competition of bacteria. Meanwhile, hydrogenotrophic methanogens were enriched in the AD reactors with MOFs. After the addition of MOFs, there was 3.28 times and 3.41 times increase in the abundance of metabolic functions related to methanogenesis by CO 2 reduction with hydrogen and dark hydrogen oxidation, respectively. There was an increased abundance of all genes that encode the key enzymes used in methane metabolism. However, functional genes involved in nitrate reduction had their expressions inhibited. The work may offer a contribution to helping the industry achieve the carbon capture and utilization policy., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

In the present study, a biochemical strategy for improving propionic acid production from kitchen waste acidification by bioaugmentation with Propionibacterium acidipropionici (P. acidipropionici) was investigated. When the inoculum of P. acidipropionici was 30% (w/w) of the seeding sludge, the propionic acid production increased by 79.57%. Further, bioaugmentation improved the relative abundance of Firmicute and Actinobacteria. The results of metagenomic analysis further reveal that the ATP-binding cassette (ABC) transporters and all related pathways of Propanoate metabolism (ko00640) were enriched when P. acidipropionici was added. For Propanoate metabolism, most functional genes involved in the conversion from Glycolysis / Gluconeogenesis (ko00010) to Propanoyl-CoA and conversion from Propanoyl-CoA to propionic acid were enhanced after bioaugmentation with P. acidipropionici, thereby promoting propionic acid production. As such, bioaugmentation with P. acidipropionici was effective in the anaerobic acidification of kitchen waste for propionic acid production., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

In this study, the integration of the electrocoagulation (EC) process with anaerobic digestion as a novel in-situ biomethanation approach was considered for the first time. As a result of this integration (iron electrodes, current density of 1.5 mA/cm 2 and an exposure mode of 10-min-ON/ 30-min-OFF), the carbon dioxide content of biogas reached below 2%. Also, the methane production rate improved by 18.0 ± 0.4%, whereas the removal efficiencies of chemical oxygen demand, turbidity, phosphate, and sulfate increased by 12.0 ± 1.5%, 30.7 ± 1.7%, > 99%, and 75.7%, respectively. Anaerobic granular sludge characteristics were also improved. Moreover, the EC process stimulated growth and quantity of functional microorganisms, especially Acinetobacter in bacterial and Methanobacterium in archaeal community. Methane concentration, however decreased due to possible excess hydrogen production. The application of the biogas as bio-hythane, and the optimization of the hybrid bioreactor to decrease hydrogen production, are possible avenues for further research., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Algal-bacterial granular sludge (ABGS) is an energy-saving and environment-friendly wastewater treatment technology; however, the effects of microplastics (MPs) on the performance and stability of the ABGS system remain unknown. Herein, the influencing mechanisms of polystyrene MPs (50 μm) on the ABGS were systematically investigated. The ABGS exhibited a high removal efficiency of MPs (over 96%) at 1 mg/L and 20 mg/L. Although the biomass content, sludge settling and particle size were not obviously affected by MPs, the COD and total phosphorus (TP) removal efficiencies were inhibited by 2.6%-4.1% and 2.9%-5.8%, respectively. Meanwhile, the structural stability of ABGS was damaged by MPs, owing to the excessive oxidative stress, low content of protein-like substance (especially tryptophan and tyrosine), and the large portion of loose protein secondary structure. Microbial community analysis revealed that the relative abundance of some functional bacteria (Candidatus&#95;Competibacter and Rhodobacter) and algal species (Tetradesmus) were decreased under the MPs stress., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Thermophilic biological nitrogen removal would be a promising alternative to conventional approaches for the treatment of high-temperature wastewater. In this study, the response of thermophilic denitrification system (50 °C) to a wide range of pH (3-11) was investigated. The results showed that thermophilic denitrification could adapt to pH 5-11, but suffered from obvious nitrite and ammonia accumulation at pH 3. Microbial insights indicated that the enrichment of specific functional thermophiles has contributed to the tolerance towards unfavorable pH. Besides, the potential selecting advantage of nitrate reducing bacteria over nitrite reducing bacteria and the enrichment of dissimilatory nitrate reduction to ammonium (DNRA) bacteria could be responsible for the nitrite and ammonia accumulation at pH 3. Moreover, the functional gene prediction denoted higher narG/(nirK + nirS) and nrfA at pH 3, which could facilitate partial denitrification and DNRA. These findings could provide new insight into the application of thermophilic biological nitrogen removal., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Low hydrogen (H 2 ) yield via dark fermentation often occurs, being mainly due to H 2 generation pathway shift. In this study, lanthanum-iron oxide nanoparticles (LaFeO 3 NPs) were prepared to investigate their effects on bioH 2 production. The highest H 2 yield of 289.8 mL/g glucose was found at 100 mg/L of LaFeO 3 , being 47.6% higher than that from the control (196.3 mL/g glucose). The relative abundance of Firmicutes increased from 54.2% to 67.5%. The large specific surface area of LaFeO 3 provided sufficient sites for the colonization of Firmicutes and increased the bacterial access to nutrients. Additionally, the La 3+ gradually released from LaFeO 3 NPs raised microbial transmembrane transport capacity, promoting glycolytic efficiency and Fe availability, thereby increasing hydrogenase content, and shifting the bioH 2 evolution to butyrate pathway for more H 2 . This provides the novelty for biochemical utilization of La and new insights into the improved H 2 yield amended with LaFeO 3 ., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Promoting direct interspecies electron transfer (DIET) with conductive additives has proved effective in improving anaerobic digestion performance and stability. However, its application is limited by the need to replenish the washout loss of conductive materials. This study reports the formation of conductive magnetite-embedded granular sludge and its long-term influence on the performance of upflow anaerobic sludge blanket reactors treating dairy wastewater. The magnetite-supplemented reactor maintained better performance than the no-magnetite control, with greater sludge settling and electron transport activity, throughout the 192-d experiment at increasing organic loading rates (1.2-8.5 g chemical oxygen demand/L·d). The abundance of electroactive microbes also remained higher in the magnetite-supplemented reactor. The results suggest that DIET-based electric syntrophy was promoted in the magnetite-embedded granules. This study is the first to demonstrate the self-embedment of submicron conductive material into granular sludge and its benefits. These findings offer a new approach to enhancing anaerobic granular sludge systems., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

The effects of static magnetic field (SMF) on an anoxic/oxic sequencing batch reactor were investigated from the perspective of electron transport via determining the variations of reduced/oxidized nicotinamide adenine dinucleotide (NADH/NAD + ) ratio, NADH concentration, electron transport system activity (ETSA), poly-β-hydroxybutyrate (PHB), extracellular polymeric substances (EPS), as well as the gene expression under different conditions. Moreover, the shifts of microbial community were also analyzed. The application of SMF with an appropriate intensity significantly improved the performance of the process, the abundance of the anoxic denitrifiers, and the activity of the aerobic denitrifiers. The NADH content, as well as ETSA were also enhanced, therefore, the total nitrogen removal efficiency of the process was increased. However, the overhigh SMF intensity resulted in the change of microbial community, meanwhile, had negative effects on the metabolism of microorganisms. Selecting a proper intensity is crucial for the SMF-enhanced biological wastewater treatment process., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

To improve the efficiency of methane production from chicken manure (CM) anaerobic digestion, the mechanism of coal slime (CS) as an additive on methane production characteristics were investigated. The results showed that adding an appropriate amount of CS quickened the start of the fermentation and effectively increased the methane yield. In addition, the pH changed in a stable manner in the liquid phase, and the concentrations of total ammonia nitrogen (TAN) and free ammonia nitrogen (FAN) were reduced. Moreover, organic matter was decomposed and volatile fatty acids (VFAs) were consumed effectively. The abundance of Bacteroides in the bacterial community and Methanosarcina in the archaea was increased. In addition, the reduction of CO 2 was the main methanogenic pathway, and adding CS raised the abundance of genes for key enzymes in metabolic pathways during methane metabolism. The results provide a novel method for the efficient methane production from CM., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Microbiota quorum sensing (QS) induced by 3O-C6-HSL (N-(β-ketocaproyl)-DL-homoserine lactone) inhibited the calcification of anaerobic granular sludge (AnGS), and the mechanism of promoting the activity recovery of calcified AnGS was studied in this paper. Through research, it was speculated that 3O-C6-HSL acted on calcified AnGS residual microorganisms to trigger QS. It enriched many functional microorganisms. For example, it promoted the growth of Methanospirillum. The CO 2 could be consumed quickly by Methanospirillum and reduced the calcium carbonate formation. The increase of microbial biomass would promote the activity of sludge. What's more, the pore size and porosity of sludge would increase, so the mass transfer channel will be broadened at same time. All those, could help the calcified AnGS quickly restore the activity and anaerobic system recover normal, which provided a new idea for the emergency rescue of anaerobic system in the future., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Thermophilic biological nitrogen removal will provide low-cost strategies for the treatment of high-temperature nitrogenous wastewater (greater than 45 ℃). In this study, a thermophilic denitrifying granular sludge system was established at 50 ℃ and compared with mesophilic systems (30 ℃ and 40 ℃). The results showed a significant increase in COD and nitrate removal rate with the elevating temperature. Besides, the microbial community analysis indicated an obvious succession of key functional bacteria at different temperatures. Enriched thermophiles including Truepera, Azoarcus, and Elioraea were the dominant denitrifiers in the thermophilic denitrifying granular sludge system, which ensured the high nitrate removal at 50 ℃. Moreover, the functional gene prediction also denoted an enrichment of nitrate reduction genes and carbon metabolism pathways at 50 ℃, which could explain the enhancement of thermophilic denitrification. These findings could provide new insight into the application of denitrifying granular sludge in thermophilic wastewater treatment., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Cassava alcohol wastewater (CAW) was utilized as a mixed feedstock to explore whether the addition of CAW could improve the anaerobic digestion of Molasses alcohol wastewater (MAW). The result showed that the rate of removal of the soluble chemical oxygen demand in the M treatment mixed with CAW was 70.13 ± 0.16%, which was significantly higher than that of the C treatment (only MAW), which was 61.23 ± 0.36%. Co-digestion in the M treatment resulted in higher methane production, achieving 23.89% increase in methane yield compared to C treatment. The addition of CAW helps to alleviate the accumulation of volatile fatty acids (397.06 ± 141.82 mg·L -1 ), enhance the stability of system and promote the establishment of stable and active microbial communities. Microbial community structure analysis indicated that hydrolytic bacteria such as Bacteroidetes, Firmicutes, and Proteobacteria, and acetoclastic methanogens, including Methanosaeta and Methanosarcina were more abundant in the co-digests., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

This research investigated for the first time the influence of the single fractions (proteins, lipids, starch, cellulose, fibers and sugars) composing Household Food Wastes on Volatile Fatty Acids (VFA). A production at different pH (uncontrolled, 5.5 and 7.0): both the amount and profile of VFA were investigated. It was found that fractions rich in proteins and starch led to the greatest VFA productions (12-15 g/L), especially at neutral pH condition. On the contrary, fractions rich in cellulose, fibers, and sugars showed a very low VFA production (<2 g/L). The chemical nature of HFW influenced the speciation of the microbial communities too. Lactobacillaceae family was highly represented in proteins-, starch-, fibers and sugars-rich substrates and Atopobiaceae, Eggerthellaceae, Acidaminococcaceae and Veillonellaceae displayed positive correlation to VFAs production. Instead, Comamonadaceae showed high relative abundance in lipids- and cellulose-rich fraction and was negatively correlated to the VFAs generation., (Copyright © 2021 Elsevier Ltd. All rights reserved.)