1. Microbicidal gentamicin-alginate hydrogels.
- Author
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Kondaveeti S, Bueno PVA, Carmona-Ribeiro AM, Esposito F, Lincopan N, Sierakowski MR, and Petri DFS
- Subjects
- Carbodiimides chemistry, Chitosan chemistry, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Pseudomonas aeruginosa drug effects, Spectroscopy, Fourier Transform Infrared, Staphylococcus aureus drug effects, Thermogravimetry, Alginates chemistry, Anti-Infective Agents chemistry, Gentamicins chemistry, Hydrogels chemistry
- Abstract
Sodium alginate (Alg) reacted with antibiotic gentamicin sulfate (GS) in an aqueous-phase condition mediated by carbodiimide chemistry, in the molar ratios Alg: GS of (1:0.5), (1:1) and (1:2). The Alg-GS conjugated derivatives were characterized by elemental analysis for nitrogen content, Fourier transform infrared spectroscopy in the attenuated total reflection mode (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), thermogravimetric analyses (TGA) and water sorption measurements. XPS and FTIR-ATR analyses clearly indicated that GS molecules covalently attached to the backbone of the alginate chains by amide bond formation. The highest amount of GS bound to Alg (43.5 ± 0.4 wt%) and the highest swelling ratio (4962 ± 661%) were observed for the Alg-GS (1:2) sample. Bioluminescence assays with Pseudomonas aeruginosa PAO1/lecA:lux and colony forming counting of Staphylococcus aureus and Escherichia coli upon contact with all Alg-GS conjugates revealed microbicidal activity; however, Alg-GS (1:2) was the most efficient, due to the highest GS content., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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