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The present study assessed the nutritional composition of coffee silverskin (CSS) obtained from arabica roasted coffee. Following validated analytical methods, CSS resulted to be a high source of proteins (14.2 g/100 g) and dietary fibers (51.5 g/100 g). Moreover, the mineral analysis revealed high contents of calcium (1.1 g/100 g) and potassium (1.0 g/100 g). To date, this study provided the widest mineral profile of CSS with 30 minerals targeted including 23 microminerals with high levels of iron (238.0 mg/kg), manganese (46.7 mg/kg), copper (37.9 mg/kg), and zinc (31.9 mg/kg). Moreover, vitamins B 2 (0.18-0.2 mg/kg) and B 3 (2.5-3.1 mg/kg) were studied and reported for the first time in CSS. β-sitosterol (77.1 mg/kg), campesterol, stigmasterol, and Δ5-avenasterol, were also observed from the phytosterol analysis of CSS with a total level of 98.4 mg/kg. This rich nutritional profile highlights the potential values of CSS for innovative reuses in bioactive ingredients development., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

This study sought to determine the content of phytosterols (PS) in spent coffee grounds (SCG) to assess a novel industrial application of this coffee by-product as a commercial source of PS. Four PS were extracted and analyzed from 14 SCG samples from 12 countries. Sonication in acidic conditions followed by saponification resulted determinant for PS extraction. PS were identified by high-performance liquid chromatography-diode array detector (HPLC-DAD) after derivatization. In the order of abundance, the PS were β-sitosterol (188.5-688.5 mg kg -1 ), campesterol (48.6-214.5 mg kg -1 ), stigmasterol (58.9-188.5 mg kg -1 ), and cycloartenol (14.6-65.8 mg kg -1 mg kg 1 ). Total PS fraction ranged from 343.4 mg kg -1 to 1146.3 mg kg -1 of SCG with an average level of 618.2 ± 195.4 mg kg -1 . Considering the millions of tons of SCG produced every year, this by-product could be a sustainable source of PS to fill a growing global demand., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Pulses are an important source of proteins, carbohydrates, and dietary fibre, and also contain polyphenols, which are bioactive compounds with antioxidant activity. A new analytical method that uses high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for the quantification of sixteen polyphenols in thirty-one pulse varieties. Different extraction procedures were tested (acidic hydrolysis, alkaline hydrolysis and extraction without hydrolysis), and acidic hydrolysis at pH 2, extraction temperature of 20 °C and extraction time of 2 h was proven to be the best in terms of recovery percentages (99.7-107.6%). The highest polyphenol levels were found in beans, particularly black beans (459 mg kg -1 ) and ruviotto beans (189 mg kg -1 ); significant levels of polyphenols were also observed in lentils, particularly black lentils (137 mg kg -1 ) and quality gold lentils (132 mg kg -1 ). This study provides new information about legume polyphenols, offering reasons to promote legumes as part of a healthy diet., (Copyright © 2018 Elsevier Ltd. All rights reserved.)