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Due to the lack of innovative valorization strategies, berry pomaces are a poorly utilized as a cheap source of valuable nutrients and phytochemicals. An effective biorefining scheme was developed to recover functional components from lingonberry pomace by consecutive supercritical CO 2 (SFE-CO 2 ), pressurized liquid (PLE) and enzyme assisted (EAE) extractions. SFE-CO 2 at optimized parameters yielded 11.8 g/100 g of lipophilic fraction, containing 43.3 and 37.4% of α-linolenic and linoleic fatty acids, respectively. The combined PLE with ethanol and water additionally recovered 61.8 g/100 g of polar constituents and reduced the antioxidant capacity of starting material by up to 94%. The major portion of the antioxidants (89-94% in different assays), anthocyanins (231 mg/100 g pomace) and proanthocyanidins (15.9 g/100 g pomace) was present in PLE-EtOH extract. Cyanidin-3-galactoside was the major anthocyanin (146.9 mg/100 g). High-pressure fractionation was more efficient for obtaining bioactive pomace constituents as compared with conventional and enzyme-assisted extractions., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

In this study supercritical carbon dioxide (SFE-CO 2 ) and pressurized liquid (PLE) extractions were optimized for the recovery of valuable fractions from blackberry pomace. Consecutively applied SFE-CO 2 and PLE at optimized parameters yielded 9.9, 26.3 and 5.1 g/100 g of CO 2 , ethanol (EtOH) and water-soluble extracts, respectively. Oil of lipophilic fraction was composed mainly of healthy polyunsaturated fatty acids (linoleic 64.1%, α-linolenic 12.9%), while polar solvents effectively recovered antioxidants (up to 29.1 mg gallic acid and 168.7 mg Trolox equivalents from g pomace). PLE-EtOH extract contained 12.2 mg/g of cyanidin-3-glucoside, while other anthocyanins were detected in significantly lower quantities (0.5-0.7 mg/g). SFE-CO 2 and PLE reduced the antioxidant capacity of starting plant material by 86-93%. In terms of extraction time, solvent consumption, total yields, and phytochemical characteristics, high-pressure fractionation was more efficient for obtaining valuable pomace constituents as compared to conventional and enzyme-assisted extractions., (Copyright © 2019 Elsevier Ltd. All rights reserved.)