1. Ni(OH)2/NGQDs-based electrochemiluminescence immunosensor for prostate specific antigen detection by coupling resonance energy transfer with Fe3O4@MnO2 composites.
- Author
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Zhu, Wenjuan, Saddam Khan, Malik, Cao, Wei, Sun, Xu, Ma, Hongmin, Zhang, Yong, and Wei, Qin
- Subjects
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ELECTROCHEMILUMINESCENCE , *QUENCHING (Chemistry) , *COMPOSITE materials , *DOPING agents (Chemistry) , *QUANTUM dots - Abstract
An efficient quenching electrochemiluminescence (ECL) immunosensor based on ECL resonance energy transfer (ECL-RET) was studied for sensitive analysis of prostate specific antigen (PSA). In this protocol, nitrogen-doped graphene quantum dots (NGQDs) which could produce excellent ECL emission, were loaded onto Ni(OH) 2 with the three-dimensional (3D) hierarchical and stacked lamellar structure. Taking advantages of high conductivity and large specific surface area of Ni(OH) 2 , the better electrochemical and ECL behavior of Ni(OH) 2 /NGQDs were presented. As another part of immunosensor, the Fe 3 O 4 @MnO 2 composites with wider ultraviolet absorption range were successfully assembled. The absorption spectra of Fe 3 O 4 @MnO 2 precisely overlapped with the ECL spectra of NGQDs, leading to the significant decrement of ECL signal. Herein, the sandwich-type immunosensor was developed based on the quenching mechanism between NGQDs (ECL donor) and Fe 3 O 4 @MnO 2 (ECL acceptor). The system was optimized to realize reliable determination of PSA concentration within the linear range of 10 −5 –10 ng/mL and a detection limit of 5 fg/mL (S/N = 3). Eminently, the developed method was successfully applied in real serum samples with good recoveries in a range from 94.0% to 102%, indicating the good accuracy of the proposed method for PSA detection. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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