Search

A fluorescent probe ( 1 ) for distinguishing amongst biothiols, including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), is developed based on different cascade reactions. The key design feature of fluorescent probe 1 is the integration of two potential reaction groups for the thiol and amino groups of biothiols in one molecule. By reacting with the halogen atom and α, β-unsaturated malonitrile in probe 1 , Cys, Hcy and GSH can generate a total of three main products with distinct photophysical properties. Probe 1 shows a strong fluorescence turn-on response to Cys with blue–green emission by using an excitation wavelength of 390 nm. At an excitation wavelength of 500 nm, probe 1 responds to GSH over Cys and Hcy and emits strong orange fluorescence. The discrimination of biothiols can be demonstrated by cell imaging experiments, indicating that probe 1 can be a useful tool for the selective imaging of Cys and GSH in living cells. [ABSTRACT FROM AUTHOR]

• A colorimetric and fluorescent probe QLB-CF for Hydrogen polysuldes was reported. • The probe achieved the fast naked-eye detection to H 2 S n and the detection limit as low as 0.03μM. • The QLB-CF was successfully applicable to exogenous H 2 S n detection and imaging in living cells. A colorimeric and fluorescent probe QLB-CF has been developed for the highly sensitive and specific imaging of hydrogen polysuldes (H 2 S n , n> 1). Its selective fluorometric/colorimetric sensing of H 2 S n was demonstrated in detail. Upon addition of H 2 S n , the probe exhibited fast response to H 2 S n along with a remarkable color change from colorless to yellow which could be easily observed by the naked eye. Similarly, the probe exhibited fluorescence response to H 2 S n with approximately 7-fold fluorescence enhancement and the fluorescence color change from none to cyan. In addition, the probe displayed high selectivity towards H 2 S n among various analytes. What's more, the probe has been successfully applied to exogenous H 2 S n detection and imaging in living cells, allowing us to visualize its sensing potential application in living cells. [ABSTRACT FROM AUTHOR]