1. Ultrasensitive competitive method-based electrochemiluminescence immunosensor for diethylstilbestrol detection based on Ru(bpy)32+ as luminophor encapsulated in metal–organic frameworks UiO-67.
- Author
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Dong, Xue, Zhao, Guanhui, Liu, Li, Li, Xuan, Wei, Qin, and Cao, Wei
- Subjects
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ELECTROCHEMILUMINESCENCE , *DIETHYLSTILBESTROL , *BIOSENSORS , *PHOSPHORS , *ENCAPSULATION (Catalysis) , *METAL-organic frameworks - Abstract
In this work, Ru(bpy) 3 2+ encapsulated in metal–organic frameworks (MOFs) UiO-67 (Ru(bpy) 3 2+ /UiO-67) as luminophor was easily prepared and firstly applied in constructing an electrochemiluminescence (ECL) immunosensor to efficiently estimate diethylstilbestrol (DES). The competitive method-based ECL immunosensor platform was fabricated by amino-silicon dioxide which possesses large surface area. The poriness of UiO-67 was splendid so that Ru(bpy) 3 2+ could be easily encapsulated. Ru(bpy) 3 2+ /UiO-67 with excellent ECL luminescence signal existed large specific surface area for easily labeled with antibodies. DES competed with bovine serum albumin-diethylstilbestrol (BSA-DES) for binding to antibody-specific sites in the constructed immunosensor. However DES was micromolecule, which was easier to bond to antibodies than BSA-DES. The ECL signal was gradually decreases with the increase of the concentration of DES. Under optimal conditions, the proposed immunosensor exhibited a wide linear range from 0.01 pg mL −1 to 50 ng mL −1 with a low detetion limit of 3.27 fg mL −1 (S/N = 3). The novel fabricated immunosensor with interference immunity and high stability may cause an attractive approach for the other targets determination. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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