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The construction of advanced systems that can accurately detect neuron-specific enolase (NSE) is critical to the rapid diagnosis of small cell lung cancer (SCLC). Herein, a luminol-based electrochemiluminescence (ECL) biosensor enhanced by reactive oxygen species (ROS) is proposed to perform the ultrasensitive detection of NSE. D-Fe 2 O 3 @Pt, synthesized as a signal indicator, was combined with luminol to significantly shorten its electron transfer pathway. Its peroxidase activity catalyzed the decomposition of H 2 O 2 to generate a large amount of •OH, thus considerably increasing the ECL signal of the luminol–H 2 O 2 system. CePO 4 /CeO 2 heterostructures with improved surface-active areas were then employed as sensing substrates. The platform enabled the accelerated generation of O 2 •− through enriched Ce4+/Ce3+ redox pairs, thereby amplifying the strength of the response of the foundation. Through integrated dual ROS amplification, the proposed sandwich ECL immunosensor configuration achieved sensitive detection in the detection range of 76 fg/mL – 100 ng/mL, with a detection limit of 72.4 fg/mL. Furthermore, the sensor exhibited high selectivity for the determination of NSE in human serum. Overall, this study serves as an important reference for integrating ROS and enzymatic strategies in ECL research to achieve accurate, sensitive, and highly selective detection of a target. [ABSTRACT FROM AUTHOR]

Development of a simple, cost-effective and sensitive biosensing strategy is highly desirable to advance the applications in Alzheimer's disease diagnosis. In this paper, we present a simple, label-free and signal-on electrochemiluminescence (ECL) aptasensor for the detection of amyloid-beta (Aβ) peptide using luminol as ECL emitter and in-situ generated reactive oxygen species (ROS) as coreactant via catalytic reaction between Cu2+-Aβ and the dissolved O 2 in the presence of ascorbic acid (AA). Aβ 16 , the binding site of Cu2+ in the monomeric full-length Aβ, was used as a model in present study. As a result, this signal-on ECL aptasensor has exhibited favorable analytical performance for Aβ 16 monomer with a linear range of 1.0 × 10-13 mol/L-1.0 × 10-8 mol/L and a limit of detection of 3.5 × 10-14 mol/L (S/N=3). Furthermore, the proposed biosensor was also able to detect the full length Aβ 40 not only in the phosphate buffer saline (PBS) solution but also in human serum. The presented biosensor represents a promising, simple, turn-on and label-free diagnostic tool for blood analysis. • A simple, label-free and signal-on ECL aptasensor for the detection of Aβ peptide was development. • The aptasensor can monitor the aggregation process of Aβ 16 monomer accelerated by Cu2+. • The proposed biosensor was able to detect the full length Aβ 40 not only in the PBS solution but also in human serum. [ABSTRACT FROM AUTHOR]