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Trehalose is the major "blood sugar" of insects and it plays a crucial role in energy supply and as a stress protectant. The hydrolysis of trehalose occurs only under the enzymatic control of trehalase (Treh), which plays important roles in growth and development, energy supply, chitin biosynthesis, and abiotic stress responses. Previous reports have revealed that the vital hormone 20-hydroxyecdysone (20E) regulates Treh, but the detailed mechanism underlying 20E regulating Treh remains unclear. In this study, we investigated the function of HaTreh1 in Helicoverpa armigera larvae. The results showed that the transcript levels and enzymatic activity of HaTreh1 were elevated during molting and metamorphosis stages in the epidermis, midgut, and fat body, and that 20E upregulated the transcript levels of HaTrehl through the classical nuclear re-ceptor complex EcR-B1/USP1. HaTreh1 is a mitochondria protein. We also found that knockdown of HaTreh1 in the fifth- or sixth-instar larvae resulted in weight loss and increased mortality. Yeast two-hybrid, coimmunoprecipitation, and glutathione-S-transferase (GST) pull-down experiments demonstrated that HaTreh1 bound with ATP synthase subunit alpha (HaATPs-α) and that this binding increased under 20E treatment. In addition, 20E enhanced the transcript level of HaATPs-α and ATP content. Finally, the knockdown of HaTreh1 or HaATPs-α decreased the induction effect of 20E on ATP content. Altogether, these findings demonstrate that 20E controls ATP production by up-regulating the binding of HaTreh1 to HaATPs-α in H. armigera. [ABSTRACT FROM AUTHOR]

Background: The fruit of Psoralea corylifolia L., Psoraleae fructus (PF), is widely used in traditional Chinese medicine as a well-known herbal tonic. Previous studies have shown that PF and its major constituents may have potential values in the treatment of Parkinson and Alzheimer diseases, though their pharmacokinetics and brain distribution were largely unknown.Purpose: To develop a liquid chromatographic-tandem mass spectrometry (LC-MS/MS) method for simultaneous studies of the plasma pharmacokinetics and cerebral nuclei (including cerebellum, thalamus, brainstem, hippocampus, corpus striatum and cortex) distribution in rats of eleven known PF compounds following as psoralen, isopsoralen, psoralidin, bavachin, bavachinin, isobavachin, isobavachalcone, bavachalcone, neobavaisoflavone, corylifol A, and corylin.Methods: Rats were orally administered via gavage at a single dose of PF extract at 1.2 g/kg. The eleven known PF compounds were extracted from rat plasma and cerebral nuclei at different time points, and then determined by the established LC-MS/MS method. Non-compartmental pharmacokinetic profiles were calculated, and the distribution in rat plasma and cerebral nuclei were compared.Results: The results showed that all the tested compounds were quickly absorbed into rat plasma and distributed almost evenly to the cerebral nuclei. The distribution concentrations at different nuclei varied at one determined time point, but the overall trends were basically similar to the plasma concentration-time results. Psoralen and isopsoralen, the two highest coumarins contained in PF, displayed far higher plasma concentrations (AUC0→∞, plasma≈53,884∼65,578 ng·h/ml) and central nervous system penetration (AUC0→∞, brain nuclei ≈44,659∼65,823 ng·h/g) than the prenylflavonoids (other compounds except psoralidin, AUC0→∞, plasma≈69∼324 ng·h/ml; AUC0→∞, brain nuclei ≈119∼3662 ng·h/g). However, the total brain-to-plasma ratios of the prenylflavonoids were higher than the coumarins, suggesting the prenylflavonoids can more readily enter the brain than the coumarins.Conclusion: The established LC-MS/MS method is sensitive and specific for the simultaneous quantitation of the eleven PF compounds in rat plasma and cerebral nuclei. The results of plasma pharmacokinetics and cerebral nuclei distribution may reveal the possible substance basis for the CNS activities of PF, and highlight the application possibility of PF and its major constituents in the treatment of Parkinson and Alzheimer diseases. [ABSTRACT FROM AUTHOR]

A gravitropism-deficient mutant M96 was isolated from a mutant bank, generated by ethyl methane sulfonate (EMS) mutagenesis of indica rice accession ZJ100. The mutant was characterized as prostrate growth at the beginning of germination, and the prostrate growth phenotype ran through the whole life duration. Tiller angle and tiller number of M96 increased significantly in comparison with the wild type. Tissue section observation analysis indicated that asymmetric stem growth around the second node occurred in M96 . Genetic analysis and gene mapping showed that M96 was controlled by a single recessive nuclear gene, tentatively termed as gravitropism-deficient M96 ( gdM96 ), which was mapped to a region of 506 kb flanked by markers RM5960 and InDel8 on the long arm of chromosome 11. Sequencing analysis of the open reading frames in this region revealed a nucleotide substitution from G to T in the third exon of LOC_Os11g29840 . Additionally, real-time fluorescence quantitative PCR analysis showed that the expression level of LOC_Os11g29840 in the stems was much higher than in the roots and leaves in M96 . Furthermore, the expression level was more than four times in M96 stem than in the wild type stem. Our results suggested that the mutant gene was likely a new allele to the reported gene LAZY1 . Isolation of this new allele would facilitate the further characterization of LAZY1 . [ABSTRACT FROM AUTHOR]

Myosin-5a contains two heavy chains, which are dimerized via the coiled-coil regions. Thus, myosin-5a comprises two heads and two globular tail domains (GTDs). The GTD is the inhibitory domain that binds to the head and inhibits its motor function. Although the two-headed structure is essential for the processive movement of myosin-5a along actin filaments, little is known about the role of GTD dimerization. Here, we investigated the effect of GTD dimerization on its inhibitory activity. We found that the potent inhibitory activity of the GTD is dependent on its dimerization by the preceding coiled-coil regions, indicating synergistic interactions between the two GTDs and the two heads of myosin-5a. Moreover, we found that alanine mutations of the two conserved basic residues at N-terminal extension of the GTD not only weaken the inhibitory activity of the GTD but also enhance the activation of myosin-5a by its cargo-binding protein melanophilin (Mlph). These results are consistent with the GTD forming a head to head dimer, in which the N-terminal extension of the GTD interacts with the Mlph-binding site in the counterpart GTD. The Mlph-binding site at the GTD-GTD interface must be exposed prior to the binding of Mlph. We therefore propose that the inhibited Myo5a is equilibrated between the folded state, in which the Mlph-binding site is buried, and the preactivated state, in which the Mlph-binding site is exposed, and that Mlph is able to bind to the Myo5a in preactivated state and activates its motor function. [ABSTRACT FROM AUTHOR]

The accumulation of pigments affects the color of rice hulls while only limited information is known about its underlying mechanisms. In the present study, a rice brown hull 6 ( bh6 ) mutant was isolated from an ethane methyl sulfonate (EMS)-induced IR64 mutant bank. Brown pigments started to accumulate in bh6 rice hulls after heading and reached a higher level in mature seeds. Some major agronomic traits including panicle length and 1000-grain weight in bh6 were significantly lower than those in its corresponding wild type IR64, while other agronomic traits such as plant height, growth duration and seed-setting rate were largely similar between the two genotypes. The analysis of pigment content showed that the contents of total flavonoids and anthocyanin in bh6 hulls were significantly higher than those in IR64 hulls. Our results showed that the brown hull phenotype in bh6 was controlled by a single recessive gene which locates on the long arm of chromosome 9. Sequencing analysis detected a single base substitution (G/A) at position 1013 of the candidate gene (LOC_Os09g12150) encoding an F-box domain-containing protein (FBX310). Functional complementation experiment using the wild type allele can rescue the phenotype in bh6 . Thus, we named this mutated gene as OsFBX310 bh6 , an allele of OsFBX310 functioning as an inhibitor of brown hull. The isolation of OsFBX310 bh6 and its wild type allele can provide useful experimental materials and will facilitate the studies on revealing the mechanisms of flavonoid metabolism in monocot plants. [ABSTRACT FROM AUTHOR]

• The SEM observes the failure initiation and development. • The FEM analysis predicts the failure location and stress distribution. • The optimization schemes solve the failure problem. This paper presents the failure analysis of an engine mounting bracket. During the proving ground testing, the right brackets of the three testing cars get broken or cracked at the mileage about 7,000 km. In order to determine the causes of the failure, a nonlinear finite element method (FEM) and failure morphology analysis together with mechanical properties, chemical components and micro-structural analyses of the specimen s material have been performed. The metallographic observation shows the precipitates observed at the inner and outer surfaces, which reduces the strength of inter-granular and then causes the fatigue fracture initiation at the inter-granular. The FEM results show that, the max mises stress is right just at the fillet of bracket, where is the initiation of the crack, and it is a little higher than the yield stress of SAPH400. An optimized structure is proposed, the max tress reduces 20%, and the following tests show the optimized bracket achieving the test mileage more than18,000 km and meets the test requirement. [ABSTRACT FROM AUTHOR]

Background: It is well known that percutaneous coronary intervention (PCI) in hemodialysis (HD) patients is associated with higher rates of in-stent restenosis and major adverse cardiovascular events (MACE) compared to that in non-HD patients, even if the target value in cholesterol management is achieved. Methods: To evaluate the factors that are associated with MACE in HD patients, we selected 142 HD patients (164 lesions) without acute coronary syndrome (ACS) from 2148 patients (2568 lesions) who underwent PCI in our database of the FU-Registry [UMIN000005679, Fukuoka University Hospital EC/ IRB: 10-1-08(09-105)], and compared 52 patients (53 lesions) with MACE (MACE(+)] to 90 patients (111 lesions) without MACE [MACE(-)]. Results: Total cholesterol (TC: 150 ± 30 mg/dL vs 166 ± 39 mg/dL, p < 0.05) and high-density lipoprotein cholesterol (HDL-C: 40.1 ± 14.7 mg/dL vs 47.8 ± 13.5 mg/dL, p < 0.01) levels were significantly lower in the MACE(+) group at follow-up. No significant differences were observed in other parameters, including triglyceride, low-density lipoprotein cholesterol (LDL-C; LDL-C/HDL-C ratio, and % changes in HDL-C, non-HDL-C, LDL-C), and hemoglobin Ale (US National Glycohemoglobin Standardization Program) between before and after PCI. TC, LDL-C, and non-HDL-C at the time of PCI and TC, and HDL-C at the 9-month follow-up were negatively correlated with MACE, while body mass index (BM1) [odds ratio (OR): 0.81 ; 95% confidence interval (CI): 0.68-0.95)], prior coronary artery bypass graft (CABG) (OR: 3.89; 95%CI: 1.29-12.6), and insulin use (OR: 3.17; 95%C1: 1.23-8.55) were strongly correlated with MACE in a multivariate analysis. Conclusion: BMI, CABG, and insulin use, but not LDL-C, are independent predictors of MACE in HD patients, suggesting that the application of lipid management for non-HD patients to HD patients at the time of PCI may not necessarily be beneficial for medium-term clinical outcomes. [ABSTRACT FROM AUTHOR]

With the increasing pressure to international energy supply, greater climate and environment problem related to the traditional fossil energy, many countries have decided to develop the renewable energy vigorously. Renewable energy technologies include hydro, wind, solar, biomass, ocean energy and geothermal. Different countries and regions have different choices on renewable energy technology because of their different economic, technical and environmental conditions. The academic has paid more and more attention to the evaluation and selection of renewable energy technology. This paper builds a comprehensive evaluation index system for Chinese renewable energy technology based on analytic hierarchy process (AHP) and data envelopment analysis (DEA), from economic, technology efficiency, social and environmental benefits. The result shows that wind and solar power are more suitable for Chinese economic, technical, environmental and social requirements, followed by biomass, hydropower, ocean energy, geothermal energy. [ABSTRACT FROM AUTHOR]

The ataxia telangiectasia-mutated and Rad3-related (ATR) kinase functions as a central node in the DNA damage response signaling network. The mechanisms by which ATR activity is amplified and/or maintained are not understood. Here we demonstrate that BRIT1/microcephalin (MCPH1), a human disease-related protein, is dispensable for the initiation but essential for the amplification of ATR signaling. BRIT1 interacts with and recruits topoisomerase-binding protein 1 (TopBP1), a key activator of ATR signaling, to the sites of DNA damage. Notably, replication stress-induced ataxia telangiectasia-mutated or ATR-dependent BRIT1 phosphorylation at Ser-322 facilitates efficient TopBP1 recruitment. These results reveal a mechanism that ensures the continuation of ATR-initiated DNA damage signaling. Our study uncovers a previously unknown regulatory axis of ATR signaling in maintaining genomic integrity, which may provide mechanistic insights into the perturbation of ATR signaling in human diseases such as neurodevelopmental defects and cancer. [ABSTRACT FROM AUTHOR]

The component pick-and-place sequence is one of the key factors to affect the working efficiency of the surface mounting machine in the printed circuit board assembly. In this paper, an improved Shuffled Frog-leaping Algorithm was presented by improving the basic Shuffled Frog-leaping Algorithm (SFLA) with the strategy of letting all frogs taking part in memetic evolution and adding the self-variation behavior to the frog. The objective function of component pick-and-place sequence of the gantry multi-head component surface mounting machine was established. Parameters selection is critical for SFLA. In this study, Three-way ANOVA was used in parameters analyzing of the new improved SFLA. The parameters like memeplex numbers m, the frogs' number P and local evolution numbers iPart were found having notable effects on the mounting time (time spent for components picking and placing), but the interactions among these parameters were not obvious. Multiple comparison procedures were adopted to determine the best parameter settings. In order to test the performance of the new algorithm, several experiments were carried out to compare the performance of improved SFLA with the basic SFLA and the genetic algorithm (GA) in solving the component pick-and-place sequence optimization problems. The experiment results indicate that improved SFLA can solve the optimization problem efficiently and outperforms SFLA and GA in terms of convergence accuracy, although more CPU time is undeniably needed. [ABSTRACT FROM AUTHOR]

The purpose of the present study was to assess the efficacy of the long-term results after video-assisted pulmonary vein isolation and left atrial appendage excision for lone atrial fibrillation (AF) and to determine the most significant risk factors for the long-term results. Methods From December 2006 to December 2012, 332 consecutive patients with lone AF underwent minimally invasive surgical ablation at our center. Of the 332 patients, 91, who had undergone video-assisted pulmonary vein isolation >5 years earlier, were evaluated in the present study (48 with paroxysmal AF, 21 with persistent AF, and 22 with long-standing persistent AF). The median follow-up period was 66 months. The primary endpoint was the success rate of video-assisted pulmonary vein isolation, defined as the absence of any atrial arrhythmia recurrence lasting >30 seconds at the clinical visit and on the electrocardiogram or long-term cardiac rhythm recording after discharge. Results During the follow-up period, 1 patient (1.1%) experienced a stroke and 4 (4.4%) died of noncardiac disease. At the 5-year follow-up point, 43 of 78 patients (55.1%) were in normal sinus rhythm. Of the 39 patients with paroxysmal AF and 39 with nonparoxysmal AF, 27 (69.2%) and 16 (44.1%) were in normal sinus rhythm, respectively. The results of the univariate and multivariate analyses of the preoperative risk factors for AF recurrence showed a left atrial diameter of ≥44 mm (hazard ratio, 5.56; 95% confidence interval, 1.68-18.387; P = .005) and an AF duration of ≥31.5 months (hazard ratio, 3.67; 95% confidence interval, 1.50-8.95; P = .004) were the most significant independent risk factors. Conclusions Patients with lone AF with a large preoperative left atrial diameter and long AF duration will not be suitable for video-assisted pulmonary vein isolation alone and might need to undergo ablation of the lesions. [ABSTRACT FROM AUTHOR]

The compressible fluid flow field synergy principle was presented as an effective theoretical guide to reduce the drag during compressible flow. A compressible flow field synergy model was presented based on the incompressible flow field synergy principle. A variable cross-section pipeline air of compressible flow was used to verify the model. Two specific improvement schemes were studied for practical applications, which showed a 24% and 20% reduction in the resistance. [ABSTRACT FROM AUTHOR]

Objective To investigate the in vitro effect of caffeic acid phenethyl ester (CAPE), a NF-κB inhibitor, on the apoptosis of osteoarthritic (OA) chondrocytes and on the regulation of the gelatinases matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9). Methods Annexin V-FITC/propidium iodide (PI) labeling and western blotting were used to observe and determine the apoptosis in TNFα-stimulated primary cultured osteoarthritic chondrocytes. Also, gelatin zymography was applied to examine MMP-2 and MMP-9 activities in supernatants. Results It was confirmed by both flow cytometry and western blotting that chondrocytes from OA patients have an apoptotic background. Use of CAPE in combination with 10 ng/mL of TNFα for 24 h facilitated the apoptosis. MMP-9 in the supernatant could be autoactivated (from proMMP-9 to active MMP-9), and the physiologic calcium concentration (2.5 mmol/L) could delay the autoactivation of MMP-9. The activities of MMP-2 and MMP-9 in the fresh supernatant increased significantly in response to stimulation by 10 ng/mL of TNFα for 24 h. The stimulatory effect of TNFα just on proMMP-9 was counteracted significantly by CAPE. Conclusion NF-κB could prevent chondrocytes apoptosis though its activation was attributed to the increase of proMMP-9 activity induced by TNFα (a pro-apoptotic factor). Therefore, therapeutic NF-κB inhibitor was a 'double-edged swords' to the apoptosis of chondrocytes and the secretion of MMP-9. [ABSTRACT FROM AUTHOR]

Objective Isoliquiritigenin (ISL), a licorice chalconoid, is considered to be a bioactive agent with chemopreventive potential. This study investigates the mechanisms involved in ISL-induced apoptosis in human cervical carcinoma HeLa cells. Methods Cell viability was evaluated using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Apoptosis was determined by flow cytometry using an Annexin V-FITC Apoptosis Detection Kit. The intracellular ROS levels were assessed using a 2, 7-dichlorofluorescein probe assay. The mitochondrial membrane potential was measured with the dual-emission potential-sensitive probe 5, 5', 6, 6'-tetra-chloro-1, 1', 3, 3'-tetraethyl-imidacarbocyanine iodide (JC-1). The degradation of poly-ADP-ribose polymerase (PARP) protein, the phosphorylation of PKR-like ER kinase (PERK), the phosphorylation of the α-subunit of eukaryotic initiation factor 2 (elF2α), the expression of the 78 kD glucose-regulated protein (GRP 78), and the activation of caspase-12 were analyzed via western blot analysis. Results ISL significantly inhibited the proliferation, the increase in ROS levels and apoptotic rates of HeLa cells in a concentration-dependent manner. Moreover, ISL induced mitochondrial dysfunction, caspase activation, and PARP cleavage, which displayed features of mitochondria dependent on apoptotic signals. Besides, exposure of HeLa cells to ISL triggered endoplasmic reticulum (ER) stress, as indicated by the increase in p-elF2α and GRP78 expression, ER stress-dependent apoptosis is caused by the activation of ER-specific caspase-12. Conclusion The findings from our study suggest that ISL-induced oxidative stress causes HeLa cell apoptosis via the mitochondrion-dependent and the ER stress-triggered signaling pathways. [ABSTRACT FROM AUTHOR]

Abstract: This paper responds to challenges made by city issues. A sustainable city is one that relates social,environment and economic. Based on the three areas of social progress, environmental protection and economic development, the paper gives the compound system model of eco-city construction. Methods of Factor Analysis are used to establish a evaluation system of comprehensive developmental level of eco-city construction level in china and measure the level of eco-city construction level in china. Thus conclusions were drawn:(1) most cities in China are improving in the eco-city construction level with time, but the spatial differences is still exist, especially Central and Eastern is better than Western. (2) Xi’an ranking the first in 2005 to 2007, second in 2008, showed a slight decrease in the overall state; Lanzhou ranking third in 2005 and2006, ranking the final in 2007and 2008, State of the overall downward; Xining ranking the final in2005, fourth in 2006 and 2007, third in 2008, the overall condition was improving; Yinchuan ranking second from 2005 to 2007,first in 2008, the overall condition was improving; Wulumuqi ranking in fourth 2005 and 2008, the final in 2006, second in 2007, the overall state was fluctuating. [Copyright &y& Elsevier]

Abstract: On the basis of analyzing flaws of the traditional means of traffic accident scene survey and inadequate of the existing photogrammetry technique, this paper presents a panoramic stereo photogrammetry technology applying to traffic accident survey. By employing the result of photogrammetry, the drawing module can automatically draw traffic accident scene diagrams for the accident analysis and reconstruction, so as to quickly achieve the purpose of measurement and drawing.Experiments show that the presented accident scene coordinate-machine can measure the distance and the azimuth of all the relevant objects on the accidental scene, and enjoys the advantages of high precision in measurement, convenience in operation and carry, no necessity in calibration,etc. So it can meet the traffic police''s needs of the measurement and drawing of the traffic accident scene. [Copyright &y& Elsevier]

Abstract: A fast synchronization method of short code guiding long code is discussed in a DS/FH hybrid spread spectrum TT&C system. The acquisition of frequency-hopping short code is achieved with matched filter and the acquisition of direct-sequence spread spectrum short code is achieved with FFT based on code sequence and on carrier. The simulation results indicate that acquisition time can be greatly reduced by combining the system with carrier-based FFT, code sequence-based FFT algorithm and matched filter, which is perfectly applicable to the TT&C application environment [Copyright &y& Elsevier]

Abstract: A frequency-hopping sequence based on chaotic code is designed to solve the anti-interception and anti-jamming problems of TT&C system. It is generated by an improved binary quantization-based method and ranging long code is designed according to equivalent speed/multiple periods coding method. The results of numerical analysis indicate that hybrid spread spectrum TT&C system has made great progress in terms of anti-jamming and anti-interception performance compared with the current single spread spectrum. [Copyright &y& Elsevier]

Abstract: A DS-FFH/MSK hybrid spread spectrum TT&C system is designed to improve the anti-interception and antijamming performance of TT&C system. MSK modulation signal is selected for hybrid spread spectrum TT&C system upon analysis and comparison. A synchronization method of short code guiding long code is discussed. The frame format of complete information and data of the hybrid spread spectrum is designed. The simulation results indicate that acquisition time can be greatly reduced, which is perfectly applicable to the TT&C application environment. [Copyright &y& Elsevier]

Abstract: In this paper, based on the analysis of existing embedded real-time operating system, the architecture of an operating system is designed and implemented. The experimental results show that the design fully complies with the requirements of embedded real-time operating system, can achieve the purposes of reducing the complexity of embedded software design and improving the maintainability, reliability, flexibility. Therefore, this design program has high practical value. [Copyright &y& Elsevier]

Staphylococcus epidermidis is a skin-resident bacterium and a major cause of biomaterial-associated infections. The transition from residing on the skin to residing on an implanted biomaterial is accompanied by regulatory changes that facilitate bacterial survival in the new environment. These regulatory changes are dependent upon the ability of bacteria to "sense" environmental changes. In S. epidermidis, disparate environmental signals can affect synthesis of the biofilm matrix polysaccharide intercellular adhesin (PIA). Previously, we demonstrated that PIA biosynthesis is regulated by tricarboxylic acid (TCA) cycle activity. The observations that very different environmental signals result in a common phenotype (i.e. increased PIA synthesis) and that TCA cycle activity regulates PIA biosynthesis led us to hypothesize that S. epidermidis is "sensing" disparate environmental signals through the modulation of TCA cycle activity. In this study, we used NMR metabolomics to demonstrate that divergent environmental signals are transduced into common metabolomic changes that are "sensed" by metabolite-responsive regulators, such as CcpA, to affect PIA biosynthesis. These data clarify one mechanism by which very different environmental signals cause common phenotypic changes. In addition, due to the frequency of the TCA cycle in diverse genera of bacteria and the intrinsic properties of TCA cycle enzymes, it is likely the TCA cycle acts as a signal transduction pathway in many bacteria. [ABSTRACT FROM AUTHOR]

Carbon monoxide dehydrogenase from the aerobic bacterium Oligotropha carboxidovorans catalyzes the oxidation of CO to CO2, yielding two electrons and two H+. The steady-state kinetics of the enzyme exhibit a pH optimum of 7.2 with a kcat of 93.3 s-1 and Km of 10.7 µM at 25 °C. kred for the reductive half-reaction agrees well with kcat and exhibits a similar pH optimum, indicating that the rate-limiting step of overall turnover is likely in the reductive half-reaction. No dependence on CO concentration was observed in the rapid reaction kinetics, however, suggesting that CO initially binds rapidly to the enzyme, possibly at the Cu(I) of the active site, prior to undergoing oxidation. A Mo(V) species that exhibits strong coupling to the copper of the active center (1 = 3/2) has been characterized by EPR. The signal is further split when [13C]CO is used to generate it, demonstrating that substrate (or product) is a component of the signal-giving species. Finally, resonance Raman spectra of CODH reveal the presence of FAD, Fe/S clusters, and a [CuSMoO2] coordination in the active site, consistent with earlier x-ray absorption and crystallographic results. [ABSTRACT FROM AUTHOR]

Abstract: Based on the study of the vertical sedimentary sequence, we recognize the assemblage of vertical lithology, the geochemical test result of mudstone sample, and the characteristics of logs, the sequence stratigraphic interfaces such as regional unconformity, regional tectonic regime interface, sedimentary system interface, transgression surface and so on. Benxi to Taiyuan formation formed in the epicontinental sea, which has subdued floor, so most of the sequence structures from Benxi to Taiyuan formation are duality system tract. Tectonic disturbance, sea level change, and differential compaction provide enough accommodation space for distribution of K1 sandstone and Qiaotou sandstone. At last we establish the framework and model of sequence stratigraphy from Benxi to Taiyuan formation in northeastern Ordos basin in this paper, which contains type model of ramp marginal basin and type of ramp in epicontinental sea background. [Copyright &y& Elsevier]

Metal-responsive transcription factor 1 (MTF-1) specifically binds to metal response elements (MREs) associated with a number of metal- and stress-responsive genes. Human MTF-1 contains a cysteine-rich cluster, -632Cys-Gln-Cys-Gln-Cys-Ala-Cys638-, conserved from pufferfish to humans far removed from the MRE-binding zinc finger domain and just C-terminal to a previously mapped serine/threonine-rich transcriptional activation domain. MTF-1 proteins containing two Cys→Ala substitutions (C632A/C634A) or a deletion in this region altogether (Δ(632-644)) are significantly impaired in their ability to induce Zn(II)- and Cd(II)-responsive transcription of a MRE-linked reporter gene in transiently transfected mouse dko7 (MTF-1-/-) cells in culture under moderate metal stress but retain the ability to drive basal levels of transcription in a MRE-dependent manner in vivo and in vitro. In addition, the mutated proteins respond to induction by Zn(II) or Cd(II) with nuclear translocation and MRE binding activities comparable with wild-type MTF-1. Attempts to rescue the Δ(632-644) deletion mutant phenotype by insetting similar Cys-rich sequences from Drosophila MTF-1 were unsuccessful, suggesting that the structure of this motif within intact human MTF-1, rather than the simple presence of multiple closely spaced Cys residues, is required for function. This cysteine cluster therefore functions at a step subsequent to nuclear translocation and MRE-binding DNA to naked promoter-containing DNA and appears to be specifically required for MTF-1 to activate transcription in the presence of inducing heavy metal ions. [ABSTRACT FROM AUTHOR]

Utilizing exhaust gases from engines with the goal of heat recovery is recognized as an essential solution to overcome the economic and environmental problems in the energy industry. Hence, the motivation of this study is to design an auxiliary tri-generation system for a ship based on exhaust gases from its engine and implement the smart use technique of the waste heat. Thermodynamic and exergoeconomic analyses are conducted to evaluate the proposed system considering acceptable thermodynamic assumptions. The system consists of a Kalina cycle, an ejector-booster refrigeration cycle, and a humidification dehumidification desalination unit for power, cooling, and freshwater production. A parametric analysis is conducted to illustrate the effect of some design variables on the sum unit cost of products. Also, to achieve the best design of the system, different optimized cases are evaluated based on a genetic algorithm. According to the attained results, employing exhaust gases from a ship's engine through the proposed tri-generation system enhanced the thermodynamic and cost outcomes. So, this is a cost-effective and clean production solution to improve the design of a ship. Considering the multi-objective optimization, energy and exergy efficiencies and the sum unit cost of products were 81%, 49%, and 78.6 $/GJ, correspondingly. Image 1 • An auxiliary tri-generation system producing cooling, power and freshwater is presented. • The system is designed for a ship based on exhaust gases from its engine as a prime mover. • Thermodynamic and exergoeconomic analyses are implemented to evaluate the system. • The system is optimized based on genetic algorithm in different cases. • Optimum energy and exergy efficiencies and production's cost are 53%, 58%, and 66.7 $/GJ, respectively. [ABSTRACT FROM AUTHOR]

Translational regulation is one of the decisive steps in gene expression, and its dysregulation is closely related to tumorigenesis. Eukaryotic translation initiation factor 3 subunit i (eIF3i) promotes tumor growth by selectively regulating gene translation, but the underlying mechanisms are largely unknown. Here, we show that eIF3i is significantly increased in colorectal cancer (CRC) and reinforces the proliferation of CRC cells. Using ribosome profiling and proteomics analysis, several genes regulated by eIF3i at the translation level were identified, including D-3-phosphoglycerate dehydrogenase (PHGDH), a rate-limiting enzyme in the de novo serine synthesis pathway that participates in metabolic reprogramming of tumor cells. PHGDH knockdown significantly represses CRC cell proliferation and partially attenuates the excessive growth induced by eIF3i overexpression. Mechanistically, METTL3- mediated N6-methyladenosine modification on PHGDH mRNA promotes its binding with eIF3i, ultimately leading to a higher translational rate. In addition, knocking down eIF3i and PHGDH impedes tumor growth in vivo. Collectively, this study not only uncovered a novel regulatory mechanism for PHGDH translation but also demonstrated that eIF3i is a critical metabolic regulator in human cancer. [ABSTRACT FROM AUTHOR]

Helicases play a critical role in processes such as replication or recombination by unwinding double-stranded DNA; mutations of these genes can therefore have devastating biological consequences. In humans, mutations in genes of three members of the RecQ family helicases (blm, wrn, and recq4) give rise to three strikingly distinctive clinical phenotypes: Bloom syndrome, Werner syndrome, and Rothmund-Thomson syndrome, respectively. However, the molecular basis for these varying phenotypic outcomes is unclear, in part because a full mechanistic description of helicase activity is lacking. Because the helicase core domains are highly conserved, it has been postulated that functional differences among family members might be explained by significant differences in the N-terminal domains, but these domains are poorly characterized. To help fill this gap, we now describe bioinformatics, biochemical, and structural data for three vertebrate BLM proteins. We pair high resolution crystal structures with SAXS analysis to describe an internal, highly conserved sequence we term the dimerization helical bundle in N-terminal domain (DHBN). We show that, despite the N-terminal domain being loosely structured and potentially lacking a defined three-dimensional structure in general, the DHBN exists as a dimeric structure required for higher order oligomer assembly. Interestingly, the unwinding amplitude and rate decrease as BLM is assembled from dimer into hexamer, and also, the stable DHBN dimer can be dissociated upon ATP hydrolysis. Thus, the structural and biochemical characterizations of N-terminal domains will provide new insights into how the N-terminal domain affects the structural and functional organization of the full BLM molecule. [ABSTRACT FROM AUTHOR]

We previously reported successful cryopreservation of shoot tips of potato 'Zihuabai' by three vitrification-based protocols. In the present study, cryo-injury to shoot tips and genetic stability in regenerants recovered from cryopreserved shoot tips by the three vitrification-based protocols were further investigated. The results showed that sucrose preculture caused no obviously different injuries, while dehydration with plant vitrification solution 2 (PVS2) was the step causing major damage to cells of shoot tips, regardless of the cryogenic procedures. Compared with droplet-vitrification and encapsulation--vitrification, vitrification caused the most severe injury to cells of the shoot tips, thus resulting in much longer time duration for shoot recovery and much lower shoot regrowth rate. Cells in apical dome and the youngest leaf primordia were able to survive and subsequently some of them regrew into shoots following all three vitrification-based cryopreservation procedures. Analyses using inter-simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) markers in shoots regrown from all three vitrification-based protocols did not find any polymorphic bands. The results reported here suggest that vitrification-based cryo-procedures can be considered promising methods for long-term preservation of potato genetic resources. [ABSTRACT FROM AUTHOR]

Reservoir fracturing stimulation is the key to constructing an enhanced geothermal system (EGS) for geothermal development in hot dry rock (HDR) reservoir. To clarify the crack propagation law of HDR fracturing, a 3D thermo-hydro-mechanical coupling simulation model of fracture propagation is produced based on the continuum–discontinuum element method (CDEM-THM3D). The correctness of the CDEM-THM3D model is validated by the theoretical solution of the nonisothermal soil consolidation model and Penny fracture model. Then, hydraulic fracturing numerical simulations are performed to analyse the influence of controlling variables on fracture propagation. The results indicate that the thermal tensile stress induced by injecting cold water can decrease reservoir fracture pressure and fracture extension pressure, causing an increasement in fracture width and a reduction in fracture length. Increasing thermal expansion coefficient and temperature difference enhances the effect of thermal stresses and even creates new branch fractures. A large elastic modulus favours an increase in fracture length, while large rock tensile strength and minimum horizontal stress lead to a decrease in fracture length. With increasing injection flow rate and fracturing fluid viscosity, the reservoir fracture pressure and the fracture width rise significantly, and the fracture easily breaks through the barrier of the high-stress compartment. [ABSTRACT FROM AUTHOR]

Investigates the constitutive activation of CCR5, a G protein-couple receptor for RANTES MIP-1alpha, MIP01beta and MCP-2, and CCR2 induced by conformational changes in the conserved TXP motif in transmembrane helix2. Finding that the expression of CCR5 and CCR2 in yeast and the availability of variants with autonomous signaling represent critical tools for characterizing receptor antagonists and developing approaches to block their role in human disease.

The envelope protein of Zika virus (ZIKV) exists as a dimer on the mature viral surface and is an attractive antiviral target because it mediates viral entry. However, recombinant soluble wild-type ZIKV envelope (wtZE) might preferentially exist as monomer (monZE). Recently, it has been shown that the A264C substitution could promote formation of dimeric ZIKV envelope protein (ZEA264C), requiring further characterization of purified ZEA264C for its potential applications in vaccine development. We also noted that ZEA264C, connected by disulfide bond, might be different from the noncovalent native envelope dimer on the virion surface. Because the antibody Fc fragment exists as dimer and is widely used for fusion protein construction, here we fused wtZE to human immunoglobulin G1 (IgG1) Fc fragment (ZE-Fc) for noncovalent wtZE dimerization. Using a multistep purification procedure, we separated dimeric ZEA264C and ZE-Fc, revealing that they both exhibit typical β-sheet-rich secondary structures and stabilities similar to those of monZE. The binding activities of monZE, ZEA264C, and ZE-Fc to neutralizing antibodies targeting different epitopes indicated that ZEA264C and ZE-Fc could better mimic the native dimeric status, especially in terms of the formation of tertiary and quaternary epitopes. Both ZEA264C and ZE-Fc recognize a ZIKV-sensitive cell line as does monZE, indicating that the two constructs are still functional. Furthermore, a murine immunization assay disclose that ZEA264C and ZE-Fc elicit more neutralizing antibody responses than monZE does. These results suggest that the two immunogen candidates ZEA264C and ZE-Fc have potential utility for neutralizing antibody selection and vaccine design against ZIKV. [ABSTRACT FROM AUTHOR]

Huntington's disease (HD) is a fatal neurodegenerative disorder caused by an abnormal expansion of polyglutamine repeats in the huntingtin protein (Htt). Transcriptional dysregulation is an early event in the course of HD progression and is thought to contribute to disease pathogenesis, but how mutant Htt causes transcriptional alterations and subsequent cell death in neurons is not well understood. RNA-Seq analysis revealed that expression of a mutant Htt fragment in primary cortical neurons leads to robust gene expression changes before neuronal death. Basic helix-loop-helix transcription factor Twist1, which is essential for embryogenesis and is normally expressed at low levels in mature neurons, was substantially up-regulated in mutant Htt-expressing neurons in culture and in the brains of HD mouse models. Knockdown of Twist1 by RNAi in mutant Htt-expressing primary cortical neurons reversed the altered expression of a subset of genes involved in neuronal function and, importantly, abrogated neurotoxicity. Using brain-derived neurotrophic factor (Bdnf), which is known to be involved in HD pathogenesis, as a model gene, we found that Twist1 knockdown could reverse mutant Htt-induced DNA hypermethylation at the Bdnf regulatory region and reactivate Bdnf expression. Together, these results suggest that Twist1 is an important upstream mediator of mutant Htt-induced neuronal death and may in part operate through epigenetic mechanisms. [ABSTRACT FROM AUTHOR]

High density lipoprotein (HDL) has been proposed to be internalized and to promote reverse cholesterol transport in endothelial cells (ECs). However, the mechanism underlying these processes has not been studied. In this study, we aim to characterize HDL internalization and cholesterol efflux in ECs and regulatory mechanisms. We found mature HDL particles were reduced in patients with coronary artery disease (CAD), which was associated with an increase in CC-chemokine ligand 2 (CCL2). In cultured primary human coronary artery endothelial cells and human umbilical vein endothelial cells, we determined that CCL2 suppressed the binding (4 °C) and association (37 °C) of HDL to/with ECs and HDL cellular internalization. Furthermore, CCL2 inhibited [3H]cholesterol efflux to HDL/ apoA1 in ECs. We further found that CCL2 induced CC-chemokine receptor 2 (CCR2) expression and siRNA-CCR2 reversed CCL2 suppression on HDL binding, association, internalization, and on cholesterol efflux in ECs. Moreover, CCL2 induced p42/44 mitogen-activated protein kinase (MAPK) phosphorylation via CCR2, and p42/44 MAPK inhibition reversed the suppression of CCL2 on HDL metabolism in ECs. Our study suggests that CCL2 was elevated in CAD patients. CCL2 suppressed HDL internalization and cholesterol efflux via CCR2 induction and p42/44 MAPK activation in ECs. CCL2 induction may contribute to impair HDL function and form atherosclerosis in CAD. [ABSTRACT FROM AUTHOR]