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A new process called Forcespinning™ has been developed to make nanofibers from a wide range of materials. This new method uses centrifugal force, rather than electrostatic force as in the electrospinning process. The Forcespinning™ method uses either solutions or solid materials that are solution or melt spun into nanofibers. Key parameters to control the geometry and morphology of the nanofibers include rotational speed of the spinneret, collection system and temperature. Orifices of the spinneret can have arbitrary geometric shapes to provide corresponding cross-section of nanofibers. The Forcespinning™ method has been successfully used to make nanofibers of poly-ethylene oxide, poly-lactic acid, bismuth, polypropylene, acrylonitrile-butadiene-styrene, polyvinyl pyrrolidone, and polystyrene among others. [ABSTRACT FROM AUTHOR]

Abstract: Electrification structures design for railway systems is a crucial and complex process, since it compounds plenty of infrastructure elements, design decisions, and calculation conditions. In this paper, an ontology-driven decision support system for designing complex railway portal frames is presented and developed. A knowledge-rules database has been also developed relying on experts knowledge and complying with railway standards. Our system outperforms the current portal frames design methods by decreasing construction time and costs. As a result, an intelligent computer-aided design tool is provided, thus facilitating the task of seeking for the optimal portal frame, which is geometrically and structurally feasible, and cost-effective. [Copyright &y& Elsevier]

Perilipins, the major structural proteins coating the surfaces of mature lipid droplets of adipocytes, play an important role in the regulation of triacylglycerol storage and hydrolysis. We have used proteomic analysis to identify CGI-58, a member of the α/β-hydrolase fold family of enzymes, as a component of lipid droplets of 3T3-L1 adipocytes. CGI-58 mRNA is highly expressed in adipose tissue and testes, tissues that also express perilipins, and at lower levels in liver, skin, kidney, and heart. Both endogenous CGI-58 and an ectopic CGI-58-GFP chimera show diffuse cytoplasmic localization in 3T3-L1 preadipocytes, but localize almost exclusively to the surfaces of lipid droplets in differentiated 3T3-L1 adipocytes. The localization of endogenous CGI-58 was investigated in 3T3-L1 cells stably expressing mutated forms of perilipin using microscopy. CGI-58 binds to lipid droplets coated with perilipin A or mutated forms of perilipin with an intact C-terminal sequence from amino acid 382 to 429, but not to lipid droplets coated with perilipin B or mutated perilipin A lacking this sequence. Immunoprecipitation studies confirmed these findings, but also showed co-precipitation of perilipin B and CGI-58. Remarkably, activation of cAMP-dependent protein kinase by the incubation of 3T3-L1 adipocytes with isoproterenol and isobutylmethylxanthine disperses CGI-58 from the surfaces of lipid droplets to a cytoplasmic distribution. This shift in subcellular localization can be reversed by the addition of propanolol to the culture medium. Thus, CGI-58 binds to perilipin A-coated lipid droplets in a manner that is dependent upon the metabolic status of the adipocyte and the activity of cAMP-dependent protein kinase. [ABSTRACT FROM AUTHOR]

• Cerato-platanin protein has been identified and isolated from P. salina. • Hydrophobin protein has been identified and isolated from T. pinophilus. • CPs and HFBs are extracted from a fermented culture medium with seaweed biomass. • Self-assemble of this extracted proteins were influenced by the polysaccharides' seaweed. The marine fungi Paradendryphiela salina and Talaromyces pinophilus degrade and assimilate complex substrates from plants and seaweed. Additionally, these fungi secrete surface-active proteins, identified as cerato-platanins and hydrophobins. These hydrophobic proteins have the ability to self-assemble forming amyloid-like aggregates and play an essential role in the growth and development of the filamentous fungi. It is the first time that one cerato-platanin (CP) is identified and isolated from P. salina (PsCP) and two Class I hydrophobins (HFBs) from T. pinophilus (TpHYD1 and TpHYD2). Furthermore, it is possible to extract cerato-platanins and hydrophobins using marine fungi that can feed on seaweed biomass, and through a submerged liquid fermentation process. The propensity to aggregate of these proteins has been analyzed using different techniques such as Thioflavin T fluorescence assay, Fourier-transform Infrared Spectroscopy, and Atomic Force Microscopy. Here, we show that the formation of aggregates of PsCP and TpHYD, was influenced by the carbon source from seaweed. This study highlighted the potential of these self-assembling proteins generated from a fermentation process with marine fungi and with promising properties such as conformational plasticity with extensive applications in biotechnology, pharmacy, nanotechnology, and biomedicine. [ABSTRACT FROM AUTHOR]