Search

Abstract: High-precision time synchronization between satellites and ground stations plays the vital role in satellite navigation system. Laser time transfer (LTT) technology is widely recognized as the highest accuracy way to achieve time synchronization derived from satellite laser ranging (SLR) technology. Onboard LTT payload has been designed and developed by Shanghai Astronomical Observatory, and successfully applied to Chinese Beidou navigation satellites. By using the SLR system, with strictly controlling laser firing time and developing LTT data processing system on ground, the high precise onboard laser time transfer experiment has been first performed for satellite navigation system in the world. The clock difference and relative frequency difference between the ground hydrogen maser and space rubidium clocks have been obtained, with the precision of approximately 300ps and relative frequency stability of 10E−14. This article describes the development of onboard LTT payload, introduces the principle, system composition, applications and LTT measuring results for Chinese satellite navigation system. [Copyright &y& Elsevier]

Recent advancements in super-resolution nanoscopy allowed the study of mitochondrial biology at nanoscale and boosted the understanding its correlated cellular processes those were previously poorly understood. Nevertheless, studying mitochondrial ultrastructure remains a challenge due to the lack of probes that could target specific mitochondrial substances (e.g. cristae or mtDNA) and survive under harsh super-resolution optical conditions. Herein, in this work, we have rationally constructed a pyridine-BODIPY (Py-BODIPY) derivative that could target mitochondrial membrane in living cells without interfering its physiological microenvironments. Furthermore, we found Py-BODIPY is a membrane potential independent probe, hence it is not limit to live-cell staining but also showed a strong internalization into pre-fixed and stimulus disrupted sample. Importantly, its cristae specificity and superb photostability allow the observation of mitochondrial dynamic nano-structures with an unprecedented resolution, allow demonstrating how mitochondrial 3D ultrastructure evolved under oxidative phosphorylation condition. • Py-BODIPY is a ΔΨm independent mitochondrial probe. • Py-BODIPY showed superb photostability and increased cristae resolution. • Py-BODIPY revealed mitochondrial 3D dynamics at nanoscale. [ABSTRACT FROM AUTHOR]

Lipid droplets (LDs) ultrastructure progression under proteohormone stimulation provide valuable clues for understanding conditions associated with obesity and diabetes. Current available LDs probes either alter intra-LDs environments or lack photo-resistance; thus, this research domain is poorly understood. In this work, a N–B–O type BODIPY- hexylcarbazole derivative named BoCz-Lip was rationally designed while achieving specific LDs live-cell targeting. BoCz-Lip showed minimal impact on cell viability as well as internal LDs' protein, triglyceride and cholesterol level. Along with its good photostability, it can be used to monitor LDs evolution under super-resolution nanoscopy. More importantly, we concluded how proteohormone could influence LDs ultrastructure, offering a better understanding for correlating diseases at the nanoscale. • BoCz-Lip has specific fluorescent response against LDs' phospholipids. • BoCz-Lip to uncover LDs morphology under STED nanoscopy. • BoCz-Lip showed minimal impact on cell viability as well as internal LDs' protein, triglyceride and cholesterol level. [ABSTRACT FROM AUTHOR]