1. Visualizing hydrogen sulfide in living cells and zebrafish using a red-emitting fluorescent probe via selenium-sulfur exchange reaction.
- Author
-
Zhang, Xiaoyu, Qu, Wangbo, Liu, Heng, Ma, Yingying, Wang, Linlin, Sun, Qi, and Yu, Fabiao
- Subjects
- *
HYDROGEN sulfide , *FLUORESCENT probes , *SELENIUM , *EXCHANGE reactions , *BRACHYDANIO , *BIOLOGICAL systems , *DETECTION limit - Abstract
Hydrogen sulfide (H 2 S) is an important endogenous gasotransmitter and has been implicated with a variety of biological processes. The development of an efficient method for monitor H 2 S fluctuations in biological systems is of great significance to understand its roles in physiological and pathological conditions. In this work, two red-emitting fluorescent probes SNARF-SSPy and SNARF-SeSPy for H 2 S detection with turn-on fluorescence signals were reported. Interestingly, SNARF-SeSPy exhibited excellent anti-interference via dual selenium-sulfur exchange reaction even in the presence of high concentrations of thiols, whereas SNARF-SSPy did not sense H 2 S in the same condition. Additionally, in the present of H 2 S, SNARF-SeSPy showed a rapid response and excellent sensitivity with a detection limit of 34 nM. Most importantly, SNARF-SeSPy featured low cytotoxicity and could be employed to detect and image exogenous/endogenous H 2 S in living cells and zebrafish. Image 1 • Two red-emitting fluorescent probes SNARF-SSPy and SNARF-SeSPy have been designed for efficient detection of H 2 S. • By comparing the two probes, only SNARF-SeSPy exhibited excellent anti-interference even in the presence of high concentration of thiols. • Results of imaging H 2 S in living cells and zebrafish demonstrated that SNARF-SeSPy could be employed to track exogenous and endogenous H 2 S in vitro and in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF