1. Differential regulation of gene expression and insulin-induced activation of phosphodiesterase 3B in adipocytes of lean insulin-resistant IRS-1 (−/−) mice
- Author
-
Hasegawa, Masaaki, Tang, Yan, Osawa, Haruhiko, Onuma, Hiroshi, Nishimiya, Tatsuya, Ochi, Masaaki, Terauchi, Yasuo, Kadowaki, Takashi, and Makino, Hideichi
- Subjects
- *
PHOSPHODIESTERASES , *FAT cells , *ENZYME metabolism , *ANIMAL experimentation , *BLOOD sugar , *CARRIER proteins , *COMPARATIVE studies , *ENZYMES , *ESTERASES , *FATTY acids , *GENES , *INSULIN , *INSULIN resistance , *RESEARCH methodology , *MEDICAL cooperation , *MICE , *PHOSPHOPROTEINS , *REFERENCE values , *RESEARCH , *RNA , *EVALUATION research - Abstract
Phosphodiesterase (PDE) 3B, a major isoform of PDE in adipocytes, mediates the antilipolytic action of insulin. PDE3B gene expression is generally reduced in adipocytes of either monogenic or polygenic rodent models of obese, insulin-resistance. An increased fat cell size, a common feature of obesity, could account for this reduction. Insulin receptor substrate-1 (IRS-1) (−/−) mice are lean with a reduced fat cell size and have insulin resistance due to a primary defect of insulin signaling. To determine whether the regulation of PDE3B gene expression is correlated with fat cell size, we examined this gene expression in adipose tissues of IRS-1 (−/−) mice. In IRS-1 (−/−) mice, PDE3B mRNA and protein levels were increased 1.24- and 1.35-fold those in C57BL/6J control mice, respectively. Independently, the fold induction of PDE activity by insulin (insulin-induced/basal) was 1.7-fold in control mice, but was reduced to 1.35-fold in IRS-1 (−/−) mice. Thus, PDE3B gene expression may be inversely correlated with a fat cell size, whereas insulin-induced PDE3B activation is mediated through IRS-1. [Copyright &y& Elsevier]
- Published
- 2002
- Full Text
- View/download PDF