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1. One-step generation of different immunodeficient mice with multiple gene modifications by CRISPR/Cas9 mediated genome engineering.

Author

Zhou, Jiankui, Shen, Bin, Zhang, Wensheng, Wang, Jianying, Yang, Jing, Chen, Li, Zhang, Na, Zhu, Kai, Xu, Juan, Hu, Bian, Leng, Qibin, and Huang, Xingxu

Subjects
*IMMUNODEFICIENCY, *GENETIC engineering, *EMBRYOS, *MICROINJECTIONS, *MESSENGER RNA, *LABORATORY mice, *CASPASES
Abstract

Highlights: [•] Embryo co-microinjection of Cas9 mRNA and multiple sgRNAs leads to heritable multiple gene modifications. [•] One-step generating multiple immunodeficient mouse models with multiallelic mutations. [•] Optimal concentration of Cas9 mRNA and sgRNAs improved targeting efficiency. [•] Targeting one site with two adjacent sgRNAs increases Cas9:sgRNA-mediated targeting efficiency and bi-allelic mutations. [ABSTRACT FROM AUTHOR]

Published
2014
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