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Extensive efforts are currently being devoted to the establishment of soil spectral libraries on regional, national, continent-wide and global domains. In particular, a new development goes in the direction of global harmonized soil spectral databases that shall be acquired following common standards and procedures so that they can be merged with other soil spectral libraries. The choice of standards is important, especially with respect to their behaviors when laboratory conditions, such as humidity, change. In this study, we test the application and robustness of the Internal Soil Standard (ISS) spectral re-alignment procedure on an extended number of soil samples acquired at different laboratories. In particular, a focus is placed on the comparison between two standards Lucky Bay (LB) and Wylie Bay (WB), for their performance with different humidity laboratory conditions. LB and WB are almost pure quartz sands from Australia. For this, 71 soil samples from Israel with different mineralogical background and variable soil organic matter contents are scanned at two laboratories. The scanning took place with different spectral measurement protocols and extreme diverse conditions in terms of laboratory humidity and moist. All scans are completed by the repeated scans of the two Australian white sands through all scan batches to harmonize the spectral measurements. Our results show that the ISS minimizes the visual spectral variation, aligns the minor and extreme systematic changes of the protocols, and makes them more stable. Furthermore, the LB and WB standards do not exhibit equal performance regarding to relatively dry and humid conditions. The WB standard provides more stable and satisfactory results in humid condition. However, the high performance of the LB sample in spectral correction is still observed, particularly in laboratories with lower moist. Accordingly, these analyses suggest that in the ISS re-alignment procedure, the WB sample is more reliable to be used in humid laboratory condition. • Testing the ISS method for spectral harmonization of a large number of soil spectra acquired in different laboratories • Testing the effects of different setups and extreme different laboratory humidity conditions • Comparing the performances of two standards LB and WB in the ISS re-alignment procedure • WB is recommended to be used in laboratories with high moist condition [ABSTRACT FROM AUTHOR]