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• MaskAL is software that can reduce the annotation effort for training Mask R-CNN. • MaskAL uses active learning to select images about which Mask R-CNN is uncertain. • On a broccoli dataset, MaskAL performed significantly better than random sampling. • By using MaskAL, 1400 image annotations were saved compared to random sampling. • The software is available on https://github.com/pieterblok/maskal. The generalisation performance of a convolutional neural network (CNN) is influenced by the quantity, quality, and variety of the training images. Training images must be annotated, and this is time consuming and expensive. The goal of our work was to reduce the number of annotated images needed to train a CNN while maintaining its performance. We hypothesised that the performance of a CNN can be improved faster by ensuring that the set of training images contains a large fraction of hard-to-classify images. The objective of our study was to test this hypothesis with an active learning method that can automatically select the hard-to-classify images. We developed an active learning method for Mask Region-based CNN (Mask R-CNN) and named this method MaskAL. MaskAL involved the iterative training of Mask R-CNN, after which the trained model was used to select a set of unlabelled images about which the model was most uncertain. The selected images were then annotated and used to retrain Mask R-CNN, and this was repeated for a number of sampling iterations. In our study, MaskAL was compared to a random sampling method on a broccoli dataset with five visually similar classes. MaskAL performed significantly better than the random sampling. In addition, MaskAL had the same performance after sampling 900 images as the random sampling had after 2300 images. Compared to a Mask R-CNN model that was trained on the entire training set (14,000 images), MaskAL achieved 93.9% of that model's performance with 17.9% of its training data. The random sampling achieved 81.9% of that model's performance with 16.4% of its training data. We conclude that by using MaskAL, the annotation effort can be reduced for training Mask R-CNN on a broccoli dataset with visually similar classes. Our software is available on https://github.com/pieterblok/maskal. [ABSTRACT FROM AUTHOR]