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Abstract Recently, the fluorescence detection of H 2 S in biological systems has attracted the attention of many analytical chemists. However, most of H 2 S probes showed shorter fluorescence emission (<600 nm) with fluorescence intensity change induced by H 2 S, which has some disadvantages of shallower tissue penetration and bigger autofluorescence interference. Herein, we have rationally designed a near-infrared and ratiometric fluorescent probe (YC−1) for the detection of H 2 S based on isophorone. The detection process can be monitored by naked-eye. Addition of H 2 S, YC−1 showed that the emission ratios of fluorescence intensity at 540 and 660 nm (I540/I660) with an obvious enhancement from 0.05 to 13.3. Besides, the color of YC−1 changes from pink to yellow. As a probe for H 2 S, YC−1 exhibited better selectivity, better sensitivity and lower detection limits (0.17 μM). Moreover, YC−1 showed lower toxicity to HepG−2 cells and can be used for cell imaging. Graphical abstract Image 1 Highlights • 1. A novel ratiometric and near-infrared fluorescent probe for detecting H2S was developed. • 2. The probe showed higher selectivity and lower detection limit (0.17 μM) for H2S monitored by naked-eye. • 3. The probe can be used for monitoring exogenous and endogenous H2S in HepG-2 cells. [ABSTRACT FROM AUTHOR]