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Hydrogen sulfide (H 2 S) is an important endogenous gasotransmitter and has been implicated with a variety of biological processes. The development of an efficient method for monitor H 2 S fluctuations in biological systems is of great significance to understand its roles in physiological and pathological conditions. In this work, two red-emitting fluorescent probes SNARF-SSPy and SNARF-SeSPy for H 2 S detection with turn-on fluorescence signals were reported. Interestingly, SNARF-SeSPy exhibited excellent anti-interference via dual selenium-sulfur exchange reaction even in the presence of high concentrations of thiols, whereas SNARF-SSPy did not sense H 2 S in the same condition. Additionally, in the present of H 2 S, SNARF-SeSPy showed a rapid response and excellent sensitivity with a detection limit of 34 nM. Most importantly, SNARF-SeSPy featured low cytotoxicity and could be employed to detect and image exogenous/endogenous H 2 S in living cells and zebrafish. Image 1 • Two red-emitting fluorescent probes SNARF-SSPy and SNARF-SeSPy have been designed for efficient detection of H 2 S. • By comparing the two probes, only SNARF-SeSPy exhibited excellent anti-interference even in the presence of high concentration of thiols. • Results of imaging H 2 S in living cells and zebrafish demonstrated that SNARF-SeSPy could be employed to track exogenous and endogenous H 2 S in vitro and in vivo. [ABSTRACT FROM AUTHOR]

Abstract Recently, the fluorescence detection of H 2 S in biological systems has attracted the attention of many analytical chemists. However, most of H 2 S probes showed shorter fluorescence emission (<600 nm) with fluorescence intensity change induced by H 2 S, which has some disadvantages of shallower tissue penetration and bigger autofluorescence interference. Herein, we have rationally designed a near-infrared and ratiometric fluorescent probe (YC−1) for the detection of H 2 S based on isophorone. The detection process can be monitored by naked-eye. Addition of H 2 S, YC−1 showed that the emission ratios of fluorescence intensity at 540 and 660 nm (I540/I660) with an obvious enhancement from 0.05 to 13.3. Besides, the color of YC−1 changes from pink to yellow. As a probe for H 2 S, YC−1 exhibited better selectivity, better sensitivity and lower detection limits (0.17 μM). Moreover, YC−1 showed lower toxicity to HepG−2 cells and can be used for cell imaging. Graphical abstract Image 1 Highlights • 1. A novel ratiometric and near-infrared fluorescent probe for detecting H2S was developed. • 2. The probe showed higher selectivity and lower detection limit (0.17 μM) for H2S monitored by naked-eye. • 3. The probe can be used for monitoring exogenous and endogenous H2S in HepG-2 cells. [ABSTRACT FROM AUTHOR]

• Naphthalimide-based a lysosome-targeting fluorescent probe LyNP-H 2 S for H 2 S sensing. • LyNP-H 2 S displays a significant 262-fold fluorescence intensity enhancement in the presence of H 2 S at 540 nm. • Moreover, LyNP-H 2 S can rapid image H 2 S in lysosome and inflamed tissues. Since the fluctuation of cellular hydrogen sulfide (H 2 S) is a very important third endogenously generated gaseous signaling molecule and plays a key role in the development of numerous human disorders, the real-time fluorescence detection of H 2 S in living systems has attracted plenty of interest during past decade. Although a lot of H 2 S fluorescent probes have been reported, the relationship between the physiology and pathology of H 2 S in organelles remains unclear, especially for inflammatory tissue. In this work, by adopting a weakly basic morpholine group as the lysosome-targeting site, a naphthalimide derivative as the signal reporter group and a 4-dinitrobenzene-ether (DNB) as fluorescence signal quencher and H 2 S-selective recognition moiety, we reported a new lysosome-targeting TP fluorescent probe LyNP-H 2 S for H 2 S detection and imaging in living cells and inflamed tissues. The probe LyNP-H 2 S exhibits very low fluorescence signal in the absence of H 2 S, and displays a significant 262-fold fluorescence intensity enhancement in the presence of H 2 S at 540 nm. Moreover, LyNP-H 2 S has the capability of quantitative detection of H 2 S at concentrations ranging from 0 to 12.0 μM (limit of detection = 9.8 nM), rapid response, as well as high sensitivity and selectivity toward H 2 S. Impressively, the results of living cell and inflamed tissues imaging test demonstrate that LyNP-H 2 S has the potentiality of being an ideal probe for real-time H 2 S detection in biosystems. [ABSTRACT FROM AUTHOR]