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Lacustrine-peat sediments from the Oskino-09 borehole in Western Siberia, which span the last 7.5 cal ka BP, were sampled for pollen and plant macro-remains to better understand regional climatic changes and the position of the forest-steppe border. Analysis of palynological assemblages indicate that meadow-steppe landscapes occupied the largest area in the middle of the Holocene (7.5–4.7 cal ka BP) due to reduced effective moisture during a warm climate interval. A subsequent gradual decrease in temperatures and evaporation led to an increase in effective moisture and emergence of birch groves during 4.7–2.0 cal ka BP, with short interruptions at ca. 3.3 and 2.5 cal ka BP. The expansion of pine forests and the advance of taiga to the south were recorded at ca. 2.0 cal ka BP. There is little evidence for significant human impact on forest-steppe belt evolution, and specifically evidence for anthropogenic deforestation on the southern border of the taiga is lacking. Human activities did not start to impact forests until the emergence of semi-nomadic cattle breeding in the Iron Age. A methodological finding of this paper is that we show that the Landscape Reconstruction Algorithm (LRA) is not always reliable and often overestimates forested areas. • Forests area is overestimated by quantifying landscape assessment in forest-steppe. • Largest meadow-steppe areas were due to reduced effective moisture ca 7.5–4.7 ka BP. • Intensive spread of coniferous in Western Siberian forest-steppe ca 2.0 ka BP. • Human impact on the landscape of Western Siberian forest-steppe was not significant. [ABSTRACT FROM AUTHOR]