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In previous studies about heterogeneous investment, many researchers only focus on the monodirectional effect of reputation, but ignore the reversed influence about the heterogeneity of reputation fluctuation driven by investment. Given this, considering the interplay between reputation and heterogeneous investment, we explore the evolution of cooperation in public goods game. In detail, each player's reputation situations in different groups determine its differentiated investment amounts, and in turn, the heterogeneity of reputation fluctuation is dependent on how much it invests into each group. Furthermore, we introduce two parameters, α and β , to characterize the effect degree of investment on heterogeneous reputation fluctuation and the one of reputation on heterogeneous investment, respectively. The simulation results suggest that cooperation can be boosted to some extent, especially for the larger α and β , as they magnify the interplay of reputation and heterogeneous investment. • We introduce the interplay between reputation and heterogeneous investment in the public goods game. • Reputation effects unequal investment and heterogeneous reputation fluctuation is effected by investment in turn. • We introduce parameter α to characterize the effect degree of investment on heterogeneous reputation fluctuation. • Parameter β is presented to denote the effect degree of reputation on heterogeneous investment. • Our new model can boost cooperation significantly, especially for the larger α and β. [ABSTRACT FROM AUTHOR]