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Summary In recent years, the Poyang Lake basin is in a prolonged drought which has placed immense pressure on the water resources utilization. In this paper, we explore the spatial and temporal distributions of extreme droughts in the Poyang Lake basin by using the methods of SPI (Standardized Precipitation Index) and EOF (Empirical Orthogonal Function) for the period of 1956–2009, which are influenced by regional precipitation anomalies and river–lake interaction due to water impounding of the Three Gorges Dam (TGD). The results show that: (1) the Poyang Lake basin experienced six extreme droughts during the past 60 years, which lead to decreases in streamflow from five tributary rivers down to the Poyang Lake. The droughts in the 1960s and the 2000s were the most serious ones. However, there was an increasing trend of streamflow in the upper and middle Yangtze in the 1960s, and a decreasing trend appeared in the 2000s. The decline of streamflow in the upper Yangtze reaches has lowered the water level of lower Yangtze River which has caused more outflow from the Poyang Lake to the Yangtze River; (2) the operation of the Three Gorges Dam (TGD) has altered the seasonal pattern of flow regimes in the Poyang Lake and significantly reduced the water level in the lower Yangtze River during the TGD impounding period from late September to early November; and (3) the conjunction of extreme droughts in the Poyang Lake and the upper Yangtze reaches coincided with the impounding of the TGD is the main cause of the low water level in the Poyang Lake. Although the impact of the recent droughts in the Poyang Lake and upper Yangtze reaches has played a crucial role in the low water level of Poyang Lake, more attention should be paid to its sensitivity to the influence of the large dam-induced changes in the interaction between river and lake, particularly during impounding periods. [ABSTRACT FROM AUTHOR]

• The most suitable ecological streamflow was calculated via kernel density estimation method. • Ecological drought was identified using the variable threshold method based on ecological streamflow. • Impacts of climate variability and human activities on ecological drought were quantified. • Human activities are the dominant factor aggravating the ecological drought in the Weihe River Basin. Although various studies have investigated the impacts of climate variability and human activities on drought, researches specifically analysing the impact on ecological drought are still limited. A deep understanding of the climatic and anthropogenic effects on ecological drought processes is crucial for ecological regulation and management in the changing environments. In the present study, an integrated approach for comprehensive understanding and quantification of ecological drought in rivers was proposed which first applied the nonparametric kernel density estimation (KDE) method to calculate the most suitable ecological streamflow (MSES) for a river ecosystem. Then, the variable threshold level method based on the MSES for each month and the run theory method were applied to identify the ecological drought duration and deficit volumes. Finally, a quantification approach based on hydrological model simulation was proposed to attribute the impacts of climate variability and human activities on ecological drought. The proposed approach was applied on two catchments, Xianyang (XY) and Huaxian (HX) within the Weihe River Basin (WRB) in northern China. Comparison results obtained using the two empirical methods revealed that the MSES calculated using the KDE method was reasonable and can be used for ecological drought identification. The identification results showed that both the median and upper quartile values of the drought duration and deficit volumes during the disturbed period (1991–2017) were greater than those during the undisturbed period (1961–1990). Quantification results showed that human activities were the dominant factor aggravating ecological drought in the WRB after 1990. The contribution rates of climate variability and human activities toward ecological drought variations were 25.6% and 74.4%, respectively, for the XY station and 42.7% and 57.3%, respectively, for the HX station. Although the WRB was selected as a case study, the proposed approach can also be applied to other regions to provide scientific guidance for regional ecological management. [ABSTRACT FROM AUTHOR]