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Abstract: A polysaccharide fraction was isolated from fresh Aloe barbadensis Miller leaves, which can promote the wound healing of the superficial II scald model mice. The monosaccharide composition and linkage determination were investigated by methylation and GC–MS, acetylation and GC, 13C NMR and DEPT. The results show that its glycosyl components contain d-glucose, d-galactose, d-xylose in a molar ratio of 5:5:1, and the API consists of a backbone of →2)-α-d-Galp-(1→2)-α-d-Glcp-(1→, having a branch of α-d-xylofuranosyl residue that is (1→3) linkage at O-3 of α-d-galactopyranosyl residue. It was found that the API could enhance proliferation of the human fibroblasts in vitro. The mechanisms of promotion proliferation were studied preliminarily. [Copyright &y& Elsevier]

Abstract: Two sulfated polysaccharide fractions (S1 and S2) were successfully isolated from seaweed Undaia pinnitafida and the chemical characteristics were determined. Antioxidant activities of the polysaccharide fractions were evaluated by assays of various antioxidants in vitro systems, including superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical-scavenging activity and metal chelating ability. The results showed that the two sulfated polysaccharides contained rhamnose as the major neutral sugar and present high sulfate content (33.99–34.29%). Antioxidant assays suggested that the two sulfated polysaccharide fractions (S1 and S2) possessed good antioxidant properties and had stronger antioxidant abilities than de-sulfated polysaccharides (DS-1 and DS-2). Available data obtained by in vitro models suggested that the correlation between the sulfate content and antioxidant activity was positive. [Copyright &y& Elsevier]

Abstract: Orthogonal array design (OAD) was applied for the first time to optimize ultrasonic-assisted extraction (UE) of polysaccharides from Poria cocos (PCP). An analysis of variance technique was employed as the data analysis strategy in this study. PCP were successfully extracted from P. cocos under the optimum UE conditions: 75min extraction time, 70 mesh particle size, and 90°C extraction temperature. An analytical method was developed and validated for the determination of chemical components of PCP by high performance liquid chromatography (HPLC). Wistar rats were fed for 40days with PCP. The total antioxidant status of organs was assayed by monitoring antioxidant enzyme activities. Results indicated that PCP could increased the level of GSH and activities of antioxidant enzymes in Wistar rats. These results confirm the effectiveness of PCP supplementation in detoxifying free radicals that are produced excessively in rats with cervical cancer. [Copyright &y& Elsevier]