1. A TICT-based fluorescent probe for hypochlorous acid and its application to cellular and zebrafish imaging.
- Author
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Shao, Sufang, Yang, Taorui, and Han, Yifeng
- Subjects
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FLUORESCENT probes , *CELL imaging , *BRACHYDANIO , *HYPOCHLORITES , *INTRAMOLECULAR charge transfer , *STOKES shift - Abstract
Hypochlorous acid (HOCl) is a crucial reactive oxygen species in biological systems, and its abnormal metabolism is associated with various diseases. Therefore, it is essential to accurately detect HOCl to understand its biological function and develop related therapeutic strategies for diseases. In this work, we developed a novel fluorescence probe, BCy-S , based on the twisted intramolecular charge transfer (TICT) mechanism for rapid and sensitive detection of HOCl in an aqueous solution. The probe exhibited a significant turn-on fluorescence response to HOCl, with high selectivity, rapid kinetics (less than 40 s), large Stokes shift (about 170 nm), and low detection limit (35.2 nM), based on the specific oxidation reaction of HOCl with sulfur atoms. Additionally, the outcomes of the bioimaging experiment showed that BCy-S was capable of real-time fluorescence visualization of HOCl in living HeLa cells and zebrafish. The development of BCy-S presents a new tool for investigating the physiological function of HOCl and its pathological role in diseases. [Display omitted] • A new TICT-based HOCl fluorescent probe has been designed and developed. • The probe exhibits an excellent sensitivity, large Stokes Shift and low detection limit to HOCl. • The probe could be applied for real-time fluorescence imaging of HOCl in live HeLa cells and zebrafish. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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