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Mitochondrial viscosity and ONOO− are involved in various pathological and physiological processes. Abnormal viscosity or irregular ONOO− concentration would disrupt the normal function of cells. Hence, we skillfully designed and synthesized a dual-responsive probe Mito-HC-TZ for the simultaneous detection of ONOO− and viscosity by different channels. 3-ethylbenzothiazolium was acted as fluorescent rotor to regulate the twisted intramolecular charge transfer (TICT) process; the C = C of 3-ethylbenzothiazolium linked to the xanthene core could be easily broken by ONOO−. Thus, the probe responded to viscosity and ONOO− by red and orange fluorescence channels, respectively. Mito-HC-TZ had high selectivity, low cytotoxicity and wide range of pH values. The results of cell imaging experiments showed the probe could be used to respectively monitor the viscosity and ONOO− level in HepG2 cells. [Display omitted] • A NIR probe for viscosity and ONOO− according to two channels was developed. • Mito-HC-TZ could ratiometrically respond to mitochondrial viscosity and ONOO−. • Mito-HC-TZ was applied to detect the changes of viscosity and ONOO− in cells. [ABSTRACT FROM AUTHOR]

A fluorescent probe, 1,4-diethyl-7-(3-pyridin-2-yl-acroyloyl)-1,2,3,4-tetrahydro-5-oxa-1,4-diazaanthracen-6-one (probe 1 ), with an emission in near-infrared region was developed for sensitive and selective detection of biothiols. Probe 1 is essentially non-fluorescent due to the strong intramolecular charge transfer (ICT) from the electron-donating tetrahydroquinoxaline moiety to the electron-withdrawing pyridine group. Upon the treatment with biothiols, the conjugation system was broken via Michael addition reaction between biothiols and α,β-unsaturated ketone, which inhibited the ICT process and resulted in a remarkable fluorescence enhancement with an emission maximum at 660 nm. Moreover, probe 1 was successfully applied to image intracellular thiols in living cells. [ABSTRACT FROM AUTHOR]

[Display omitted] • A novel Near-infrared Turn-on Fluorescent probe has been synthesized. • Fast response to Selenocysteine with a large turn-on Near-infrared fluorescence signal. • Low detection limit, high selectivity and sensitivity. • Capability for identification of Sec in living cells and mice. Selenocysteine (Sec) plays critical roles in many physiological and pathological processes of human beings, thus it is of great significance to develop accurate methods for detecting and visualizing Sec. Herein, a new anthracene derivative (YZ-M5) as a near-infrared (NIR) fluorescent probe for sensing Sec was designed. Based on the target-induced modulation of the intramolecular charge transfer (ICT) process of the probe, YZ-M5 can display a rapid strong NIR fluorescence enhancement at 660 nm for Sec within 5 min. The quantitative detection range for Sec is 0–40 μM with a low detection limit of 18.5 nM. YZ-M5 also showed high selectivity and sensitivity, as well as the capability for identification of Sec in living cells and mice. [ABSTRACT FROM AUTHOR]

• A simple near-infrared fluorescence probe based on dicyanoisophorone was synthesized. • This probe exhibited excellent specificity in the detection of N 2 H 4 over other amino compounds. • This probe showed excellent response performance to N 2 H 4 through distinct fluorescence enhancement. • This probe was applicable for N 2 H 4 detection in living cells and real water samples. With the widespread use of N 2 H 4 , it brings potential risks to human health. In order to determine N 2 H 4 effectively in both environment and living systems, a simple near-infrared fluorescence probe was prepared for the specific response of N 2 H 4 based on the intramolecular charge transfer (ICT) mechanism. This probe provided a "turn on" fluorescence response to N 2 H 4 with characteristics including high selectivity, rapid response (within 1 min), low cytotoxicity, and low detection limit (0.022 μM). In addition, it can be used to expediently trace N 2 H 4 in HepG2 cells and water samples. Thus, Probe 1 is a promising tool to identify the level of N 2 H 4. [ABSTRACT FROM AUTHOR]