1. Promising two-dimensional rhenium diselenide nanosheets: Facile Preparation, characterization, in vivo tumor photoacoustic imaging and photothermal therapy.
- Author
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Yuan, Yufeng, Song, Yiwan, Hu, Jianchang, Wu, Sihan, Wang, Yiping, Zhang, Xiaoying, Song, Jun, Liu, Liwei, Rao, Feng, Zeng, Shuwen, Peng, Xiao, and Qu, Junle
- Subjects
ACOUSTIC imaging ,NANOSTRUCTURED materials ,PHOTOACOUSTIC spectroscopy ,PHOTOTHERMAL effect ,RHENIUM ,PHOTOTHERMAL conversion ,PHOTOACOUSTIC effect - Abstract
Nanoagent-mediated photothermal therapy (PTT) has been considered as an useful strategy for curing cancer. Nonetheless, identifying high-quality photothermal nanoagents with excellent biocompatibility testing, superior photothermal conversion efficiency (PTCE), and high treatment efficacy, is still a key dilemma for performing practical application in biomedicine field. In our work, a novel photothermal nanoagent named two-dimensional (2D) colloidal rhenium diselenide (ReSe 2) nanosheets was exfoliated via a facile liqiud-assisted exfoliation approach. Owing to surface functionalization with poly-ethylene glycol (PEG), the obtained PEG-ReSe 2 nanosheets demonstrated good solubility and excellent biocompatibility. Notably, the PEG-ReSe 2 nanosheets showed excellent near infrared (NIR) photothermal activity. For the radiation of an NIR laser at 808 nm, an ultrahigh PTCE of 81.3 % was demonstrated. Owing to its high Re element and good photoacoustic effect, the PEG-ReSe 2 nanosheets can serve as good contrast nanoagents for in vivo photoacoustic (PA) imaging. Furthermore, the PEG-ReSe 2 nanosheets acted as excellent photothermal agents (PTAs) for performing PTT. Thanks to high photothermal effects of PEG-ReSe 2 nanosheets, in vitro photothermal effects showed that, the mortality rate of 4T1 cells was almost 100 %. Meanwhile, in vivo experiments showed that mouse tumors were 100 % ablated. More importantly, the PEG-ReSe 2 nanosheets exhibited negligible levels of toxicity, as demonstrated by in vivo cytotoxicity assays and histological analysis. Finally, changes in micro-RNA expression level was proposed to assess PTT treatment efficacy of PEG-ReSe 2 nanoagent. Interestingly, the miRNAs such as miR-125b-5p, miR-20a-5p, miR-214–3p, miR-221–3p, miR-212–3p and miR-27b-3p, were down-regulated. In addiiton, the miRNAs including miR-451a, miR200c-3p, and miR-let-7d-3p, were up-regulated. More importantly, these obtained miRNAs can work as typical indicators for screening PTT outcomes of PEG-ReSe 2 nanosheets-involved PTT. Our research suggested that, the PEG-ReSe 2 nanoagent may represent potential therapeutic nanoagents for in vivo photoacoustic imaging and cancer therapy. [Display omitted] • A novel photothermal nanoagent named PEG-ReSe 2 nanosheets with ultra-high photothermal conversion efficiency was prepared. • PEG-ReSe 2 nanosheets can work as good nanoagents for in vivo photoacoustic imaging and photothermal therapy. • Both down-regulation and up-regulation miRNAs were important biomarkers for screening the therapeutic outcomes of PEG-ReSe 2 nanosheets. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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