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The success of food and beverage production processes carried out by Saccharomyces cerevisiae and the thriving of food spoilage fungi are dependent on the ability of a cell to cope with the many environmental insults imposed during food production and preservation processes. Post-genomic approaches, especially transcriptomics, proteomics and chemogenomics, applied to S. cerevisiae made possible the unveiling of general and specific genome-wide adaptive response programs against stress induced by weak acids, ethanol, sulfite, heat and cold shock, osmotic pressure and nutrient limitation. These programs and the underlying signaling pathways are overviewed herein, highlighting the recent identification of genes and pathways found to be involved in stress response and tolerance. These are good candidate targets for genetic engineering aiming at the development of improved strains. The extension of the data gathered in S. cerevisiae to food spoilage fungi is considered. The relevance of the different genome-wide approaches in this context is also discussed. [Copyright &y& Elsevier]

Genomic information and tools are beginning to be used to increase our understanding of how organisms of all types interact with their environment. The study of the expression of all genes, at the genome, transcriptome, proteome and metabolome level, in response to exposure to a toxicant, is known as toxicogenomics. Here, we show how this new field of environmental genomics has enhanced the development of fundamental knowledge on the mechanisms behind the toxicity of and resistance to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Although 2,4-D is one of the most successfully and widely used herbicides, its intensive use has led to the emergence of resistant weeds and might give rise to several toxicological problems when present in concentrations above those recommended. This review summarizes recent mechanistic insights into 2,4-D toxicity and the corresponding adaptive responses based on studies carried out using Saccharomyces cerevisiae and Arabidopsis thaliana as model organisms. [Copyright &y& Elsevier]

The emergence of widespread multidrug resistance (MDR) is a serious challenge for therapeutics, food-preservation and crop protection. Frequently, MDR is a result of the action of drug-efflux pumps, which are able to catalyze the extrusion of unrelated chemical compounds. This review summarizes the current knowledge on the Saccharomyces cerevisiae drug:H+ antiporters of the major facilitator superfamily (MFS), a group of MDR transporters that is still characterized poorly in eukaryotes. Particular focus is given here to the physiological role and expression regulation of these transporters, while we provide a unified view of new data emerging from functional genomics approaches. Although traditionally described as drug pumps, evidence reviewed here corroborates the hypothesis that several MFS-MDR transporters might have a natural substrate and that drug transport might occur only fortuitously or opportunistically. Their role in MDR might even result from the transport of endogenous metabolites that affect the partition of cytotoxic compounds indirectly. Finally, the extrapolation of the gathered knowledge on the MDR phenomenon in yeast to pathogenic fungi and higher eukaryotes is discussed. [Copyright &y& Elsevier]