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Use of aerobic denitrifying consortia, as a new efficient tool for nitrogen removal, has attracted widespread attention because of their stable and efficient nitrogen removal ability. Yet, little attention has been given to highly efficient aerobic denitrifying consortia from mariculture environments. In this study, a natural aerobic denitrifying consortium, named C3, was obtained by an enrichment technique from a coastal mariculture area of Dongshan Bay of Fujian Province, China. In culture, it exhibited good and stable nitrate removing performance. Nitrogen mass balance indicated that 49.30% of the initial TN was converted into gaseous nitrogen and 49.63% was assimilated to biomass nitrogen. High-throughput sequencing and network analysis revealed that Pseudomonas and Fusarium were putative dominant aerobic denitrifying bacterial and fungal species, respectively, cooperating with Exophiala , Penicillium , Mortierella , Neocamarosporium , Neocosmospora , Sterigmatomyces , Thelebolus , Thermomyces , Cutaneotrichosporon and Gymnopilus to perform aerobic denitrification. In addition, four Pseudomonas strains have been successfully isolated from consortium C3, which supported the inference that Pseudomonas was the dominant contributor in the aerobic denitrifying consortium C3. These results will offer novel insights into aerobic denitrifying consortia in mariculture environments for biotechnological nitrogen pollution removal. • An aerobic denitrifying consortium C3 was screened through sub-cultures. • NO 3 − and TN removal efficiencies of C3 could reach 99.14% and 49.30%, respectively. • NO 3 − removal pathways of C3 were demonstrated by nitrogen mass balance. • Potential microbial cooperative interactions drove the nitrate removal process. • Dominant contribution of Pseudomonas in C3 was supported by the isolation of single strains. [ABSTRACT FROM AUTHOR]

Objective To analyze the prevalence and years lived with disability (YLD) from dental caries among children and adolescents and the time trends over the past two decades in Sichuan province, the largest province in west China. Methods Based on the Global Burden of Disease Study 2015 (GBD2015), which systematically assessed the epidemiological characteristics of major diseases and their transitions by country and region from 1990 to 2015, we extracted the estimated results for China. We then used the Bayesian meta-regression method to estimate the sex- and age-specific prevalences and YLDs from dental caries among children and adolescents under 15 years old in Sichuan province and compared them with global and national indicators for the same period. Results In 2015, there were almost 6 million cases of dental caries in children and adolescents (aged < 15 years) in Sichuan province, accounting for 6% of the total cases in China. For children under 5 years, the prevalence of deciduous caries was 55.9%, and the YLDs value was 10.8 per 100,000, while it was 24.3% and 5.1 per 100,000 respectively among 5- to 14-year-olds; for those aged 5 to 14 years, the prevalence of permanent caries was 21.5%, and the YLDs value was 11.5 per 100,000. From 1990 to 2015, the prevalence of dental caries for children under 5 years increased substantially, by 16.2%, and the YLDs increased by 8.7%. Among those aged 5 to 14 years, the prevalence increased and the YLDs decreased. Conclusion Dental caries remains a huge health burden in Western China. In contrast to the global and national data, the trend has increased rapidly over the past 25 years in this region. This work provides suggestions for the prevention and control for oral health in China with the policy of two-child. [ABSTRACT FROM AUTHOR]

• SSR labeled-release-recapture routines were established to evaluate stock enhancement. • Silver carps were facing population depression and genetic structure homogeny. • Potential genetic risk existed between recaptured populations by constant SE. • Urgent scientific SE breeding programs and traceability system should be constructed. Hatchery release of silver carp (Hypophthalmichthys molitrix) is an efficient strategy that has been carried out for years, and its scales expand year by year in the lower reaches of the Yangtze River (LRYR) in China. As the cultured juveniles are released into self-recruiting wild population to increase fisheries resources yields, they may potentially come along with negative impacts such as intra-inter specific completion, loss of genetic diversity and fitness, even causing genetic risk. To assess the relationship between genetic risk and stock enhancement (SE) of the silver carp in the Yangtze River (YR); microsatellite loci amplification, parentage assignment, genetic diversity, genetic differentiation and structure were conducted and testified by the help of eleven fluorescence-labeled microsatellite markers method. Results showed that silver carp populations in the YR had relatively higher genetic diversity (UHe), inbreeding coefficient (Fis), and effective population size (Ne) than breeding stock populations from eight hatcheries. By using the analysis of molecular variance (AMOVA), results showed that the percentage of variance among populations and within populations were 4.15 % and 95.85 %, respectively, which indicated that genetic structure of YR populations possibly had been changed by SE. Therefore, the present study suggests that the large-scale SE possibly resulted in negative genetic impact on silver carp population. Most importantly, an evaluation system in fisheries resource recruitment and genetic risks was established for SE and management. [ABSTRACT FROM AUTHOR]

Transplant coordinators (TCs) play a pivotal role in the entire donation process. Their professional competences have a direct impact on donation rates. However, few specific indicators are available for assessing TCs' professional competence. Therefore, it is imperative to develop an assessment index system to measure their competence. Our objective was to construct an index system for assessing TCs' professional competences. Based on a literature review and semistructured interviews, a focus group and 12 TCs were enrolled to design the questionnaires. Then, a modified Delphi method was used. Two rounds of questionnaires were distributed to 21 experts from 7 provinces across multiple research areas between October 2020 and March 2021. The data were sorted and analyzed by a focus group. A combined assessment index system, namely, APT, including 3 constructs, the admittance assessment model (henceforth A), practical operation assessment model (P), and thousand-point performance assessment model (T), was constructed. This APT index system consisted of 3 first-level items, 8 second-level items, and 34 third-level items. The APT assessment system has been unanimously recognized by experts. This index system, as a precursor form, will further lay the foundation for formation of an assessment instrument and provide references for other countries. [ABSTRACT FROM AUTHOR]

The gap between the supply and demand of organs is continuously increasing. The emergence of organ procurement coordinators (OPCs), to a certain extent, has decreased the gap. In China, a majority of OPCs work in hospitals that are qualified for transplants, namely in-hospital OPCs (Ih-OPCs). Their profession experiences are closely related to donation rates and indirectly reveal aspects that should be further improved. However, no data are available in China. We sought to explore Ih-OPCs' subjective feeling of the practice experience and provide targeted interventions for administrators. A project team was established and then interview outlines were listed by virtue of discussion and literature review. Furthermore, a total of 13 Ih-OPCs were selected as participants for interview. After that, the conversation was analyzed and encoded, and themes were finally identified according to phenomenology. A total of 3 themes including positive emotions, negative emotions, and personal needs were identified, which were furthermore divided into 7 minor themes ranging from professional identity, competence improvement, recruitment methods, quality of life, workload evaluation, career prospects, and training courses. Social support and public understanding play a pivotal role in strengthening positive emotions. Meanwhile, the administrators should put more emphasis on negative emotions and personal needs, and management system should be further optimized. [ABSTRACT FROM AUTHOR]

Ketamine, as a non-competitive antagonist of N-methyl-D-aspartate (NMDA) receptors, was originally used in general anesthesia. Epidemiological data show that ketamine has become one of the most commonly abused drugs in China. Ketamine administration might cause cognitive impairment; however, its molecular mechanism remains unclear. The glymphatic system is a lymphoid system that plays a key role in metabolic waste removal and cognitive regulation in the central nervous system. Focusing on the glymphatic system, this study evaluated the behavioral performance and circulatory function of the glymphatic system by building a short-term ketamine administration model in mice, and detected the expression levels of the 5-HT2c receptor, ΔFosb, Pten, Akt, and Aqp4 in the hippocampus. Primary astrocytes were cultured to verify the regulatory relationships among related indexes using a 5-HT2c receptor antagonist, a 5-HT2c receptor short interfering RNA (siRNA), and a ΔFosb siRNA. Ketamine administration induced ΔFosb accumulation by increasing 5-HT2c receptor expression in mouse hippocampal astrocytes and primary astrocytes. ΔFosb acted as a transcription factor to recognize the AATGATTAAT bases in the 5′ regulatory region of the Aqp4 gene (−1096 bp to −1087 bp), which inhibited Aqp4 expression, thus causing the circulatory dysfunction of the glymphatic system, leading to cognitive impairment. Although this regulatory mechanism does not involve the Pten/Akt pathway, this study revealed a new mechanism of ketamine-induced cognitive impairment in non-neuronal systems, and provided a theoretical basis for the safety of clinical treatment and the effectiveness of withdrawal. [Display omitted] • Ketamine administration causes circulatory dysfunction of glymphatic system. • Ketamine administration induces cognitive impairment in mice. • Ketamine induces ΔFosb accumulation by increasing the expression of 5-HT2c receptor. • ΔFosb acts as a transcription factor to recognize and inhibit Aqp4 expression. [ABSTRACT FROM AUTHOR]

Abstract: The North China Plain (NCP) is one of the main productive regions for winter wheat (Triticum aestivum L.) and summer maize (Zea mays L.) in China. However, water-saving irrigation technologies (WSITs), such as sprinkler irrigation technology and improved surface irrigation technology, and water management practices, such as irrigation scheduling have been adopted to improve field-level water use efficiency especially in winter wheat growing season, due to the water scarcity and continuous increase of water in industry and domestic life in the NCP. As one of the WSITs, sprinkler irrigation has been increasingly used in the NCP during the past 20 years. In this paper, a three-year field experiment was conducted to investigate the responses of volumetric soil water content (SWC), winter wheat yield, evapotranspiration (ET), water use efficiency (WUE) and irrigation water use efficiency (IWUE) to sprinkler irrigation regimes based on the evaporation from an uncovered, 20-cm diameter pan located 0–5cm above the crop canopy in order to develop an appropriate sprinkler irrigation scheduling for winter wheat in the NCP. Results indicated that the temporal variations in SWC for irrigation treatments in the 0–60-cm soil layer were considerably larger than what occurred at deeper depths, whereas temporal variations in SWC for non-irrigation treatments were large throughout the 0–120-cm soil layer. Crop leaf area index, dry biomass, 1000-grains weight and yield were negatively affected by water stress for those treatments with irrigation depth less than 0.50E, where E is the net evaporation (which includes rainfall) from the 20-cm diameter pan. While irrigation with a depth over 1.0E also had negative effect on 1000-grains weight and yield. The seasonal ET of winter wheat was in a range of 206–499mm during the three years experiments. Relatively high yield, WUE and IWUE were found for the irrigation depth of 0.63E. Therefore, for winter wheat in the NCP the recommended amount of irrigation to apply for each event is the total 0.63E that occurred after the previous irrigation provided total E is in a range of 30–40mm. [Copyright &y& Elsevier]

With abundant thermal resources, there is a long-time span for potential maize sowing in the hot subtropical monsoon region. However, with different sowing dates, maize may be exposed to elevated temperatures at different growth stages. This study aims to understand the impact of sowing date on maize growth and development and yield formation as temperatures increase. A 3-year field experiment was carried out in the typical subtropical monsoon region in China, with 11 sowing dates ranging from mid-March to late June with approximately 10-day intervals (named S1 to S11), and three main commercial varieties were included to validate the impact of sowing date. Following delayed sowing, maize plants were exposed to increased temperatures during the emergence (VE) to silking (R1) stages. Maize plants of the three varieties exhibited similar responses to delayed sowing dates in terms of growth, development, and yield performance. Vegetative growth was accelerated, while the anthesis-silking interval (ASI) and the vegetative-to-reproductive transition, that is, the interval of V12 (twelfth leaf) to R1 stages, were prolonged. The grain yield gradually decreased and reached a minimum at approximately S8-S10 (varied among varieties), which was in accordance with the decreased grain number. Further analyses revealed that the decreased grain number and yield were associated with a prolonged V12-R1 interval with delayed sowing. The duration of V12-R1 was significantly positively correlated with the maximum and minimum temperatures within a day, suggesting that the V12-R1 interval could be a parameter for evaluation of heat's impact on yield formation. Grain weight decreased as the temperature of the R1-R6 stage rose before S8 but increased as the temperature dropped after S8. Inconsistently, biomass accumulation, leaf area, and plant and ear height first increased and then decreased before and after S3-S6, respectively. Delayed sowing in hot subtropical monsoon regions exposes maize plants to elevated temperatures, leading to accelerated vegetative and reproductive growth but a prolonged vegetative-to-reproductive transition, and suppresses yield by reducing grain number and/or weight, resulting the yield decreased with the delayed sowing. By demonstrating the impacts of high temperature with different sowing dates on the phenology, morphology, and yield formation of maize, this study provides a basis for improving maize yield in practical production by optimizing the sowing date. • Optimal date for maize sowing in subtropical monsoon region in China. • Delayed sowing exposes maize to a long-term accumulative high temperature. • Delayed sowing extends vegetative-to-reproductive transition for maize development. • Delayed sowing differently reduces yield by suppressing grain number and/or weight. [ABSTRACT FROM AUTHOR]

Organ transplantation is an important method to save the lives of patients suffering from organ failure. However, the low rate of organ donation is a common problem worldwide. Many potential organ donors in the intensive care unit (ICU) are not properly identified, which is one reason for the low donation rate. ICU nurses play a key role in organ donation but may be uncertain regarding some issues. In this study, an analysis of the reasons why ICU nurses in western China are reluctant to encourage patients and their families to donate organs is performed, providing a reference for promoting ICU nurse participation in organ donation work. From August to November of 2017, using a purposive sampling method, we conducted semi-structured, in-depth interviews using a phenomenological research method with 18 ICU nurses who were working in 4 large hospitals with organ transplant accreditation in Chongqing City, China, and analyzed the data with phenomenology. Reasons for the reluctance of ICU nurses in encouraging patients to donate organs were categorized into the following 4 themes: limitation of the nurses' professional role, influence of the family's negative emotions, lack of training regarding organ donation in medical institutions, and impact of a conservative social attitude. Chinese medical and health institutions need to attach importance to the duties and roles of ICU nurses in organ donation work, the creation of a good death culture, the implementation of training for organ donation specialist nurses, and the strengthening of advocacy efforts for organ donation so that ICU nurses' reluctance in engaging in organ donation coordination in China can be mitigated and the nurses can better participate in promoting organ donation to potential donors. • To the best of our knowledge, China does not have a clear law on the duties of nurses involved in organ donation. However, there are a large number of potential organ donors in the ICU; ICU nurses are the first to identify potential organ donors. This study explored the reasons why ICU nurses refused to participate in organ donation, and thus discovered the existing problems in the implementation of organ donation policy. • There is little research on ICU nurses encouraging patients to donate organs in China. This study aimed to learn the views of ICU nurses, of which were offered as a reference for China and even the world, and to promote the role of ICU nurses in organ donation and the improvement of organ donation policy. [ABSTRACT FROM AUTHOR]

Global promotion of organ transplantation is contingent upon continuous improvement of the donation rate. Organ donation education is an important measure for changing public awareness and promoting organ donation. Therefore, it is important and urgent to conduct a systematic study of the organ donation courses offered in China and around the world. The aim of this study was to learn the views and needs of Chinese medical students and provide a reference for international peers. Semistructured, in-depth interviews and purposive sampling were used in this study. A total of 18 university students majoring in medicine and nursing were selected according to the principle of data saturation and interviewed. The data were sorted and analyzed with phenomenology. These interviewees generally agreed that an organ donation course should be offered. They were in urgent need of knowledge regarding organ donation procedures and relevant policies and eager for a practical and experience-based teaching method design. The Chinese medical students surveyed also believed that the course should also be available to clinical practitioners, nonmedical students, and the public. Medical students' demand for an organ donation course should be considered. To fully stimulate their interest in learning and to provide support and a guarantee for continuous improvement of transplant operations promotion and donation rate worldwide, specific organ donation courses and flexible training should be created. • To the best of our knowledge, there is no organ donation promotion education in China at present. This study explored the design of the content and teaching modes of organ donation education. • This research was conducted from the perspective of those who will receive the organ donation course—medical students—so as to provide a necessary foundation for the later course practice and student acceptance. [ABSTRACT FROM AUTHOR]

The H9N2 subtype of avian influenza virus (H9N2 AIV) has caused significant losses in chicken flocks throughout China. At present, consensus has been reached that field isolates of H9N2 underwent antigenic drift to evolve into distinct groups with significant antigenic divergence from the commercially available vaccines in China. This project continues to monitor the evolution characteristics of H9N2 hemagglutinin (HA) genes in China over the past 3 yr. The results showed that the current circling H9N2 viruses were diversified into h9.4.2.5 subclade, which was genetically distant from commonly used commercial vaccine strains. Compared with vaccine strains or 2014 strains, more than 42.1% of the variable antigenic sites in recent 3 yr' strains have shown significant changes and these stacked changes have caused significant differences in antigenicity. We constructed a recombinant vaccine strain rCQY-GHHA, which uses A/Chicken/China/SichuanCQY/2014 as the framework and A/Chicken/China/SichuanGH/2020 strain, which meets the recent viral antigenic characteristics, as the HA gene donor. The recombinant strain was prepared as an oil-adjuvant inactivated vaccine following an industrial process. The results of the immune protection experiment showed that the rCQY-GHHA vaccine was better than the commercial vaccine strain SS in reducing the morbidity, pathological lesion, virus shedding, and viral load. These results provide a reference for the control of H9N2 AIV in China. [ABSTRACT FROM AUTHOR]

Caprock sealing ability is one of the key geological factors to ensure the stable and safe operation of the underground gas storage (UGS). Gypsum salt rock is the high-quality caprock for oil and gas reservoirs, however, the effect of cyclic stress on its sealing capacity is still unclear, which restricts the construction progress of this kind of UGS. Therefore, taking the H UGS in the Sichuan Basin in China as an example, this paper analyzes the initial sealing capacity of gypsum salt caprock using cast thin section, conventional physical property test, nuclear magnetic resonance and breakthrough pressure tests. On this basis, study the variation characteristics of physical and mechanical parameters of gypsum salt caprock under cyclic stress using cyclic stress loading and unloading experiment, and then analyze the evolution law of its sealing capacity. The results show that gypsum salt caprocks of H UGS can be used as a good tight caprock with the porosity less than 1.0%, permeability less than 0.005 mD, breakthrough pressure greater than 6.0 MPa and triaxial compressive strength greater than 210 MPa. In addition, the physical properties of gypsum salt caprock become worse and the sealing capacity increases under cyclic stress, and physical and mechanical changes of gypsum salt caprock mainly occur in the first 30 cycles accounting for about 75%. Moreover, with the increase of cycles, the Poisson's ratio increases by 88% while the change range of elastic modulus is only 6.4%, indicating that gypsum salt caprocks mainly expands laterally and still maintain good elasticity. However, when the cycle times reach a certain threshold of 1002, the cumulative plastic strain of gypsum salt rock will become larger and larger until fracture. And the gypsum salt caprocks can be effective cover in the 184 cycles of loading and unloading with the maximum pressure threshold of 18 MPa and minimum pressure threshold of 1 MPa. This research results can provide theoretical guidance for cap rock stability analysis and operation parameter design of gas reservoir. • The variation characteristics of physical and mechanical parameters of gypsum salt caprock under cyclic stress were analyzed • The gypsum salt caprock is dominant by the compaction of pores and microcracks and mainly occur in the first 30 cycles. • The damage variables are defined from the perspective of energy, and the fatigue life of gypsum rock is 1002 times. • The sealing capacity of the gypsum salt caprock gradually increases with the process of cyclic loading and unloading. [ABSTRACT FROM AUTHOR]

• Converting natural forest to tea plantation decreased soil labile organic C. • Forest conversion decreased the relative abundance of copiotrophic bacteria. • Land-use change increased oligotrophic bacteria and lignin-degrading fungi groups. • Forest conversion decreased cellulase activity but increased ligninase activity. • Soil labile organic C mediated soil microbial community composition and function. The conversion of natural forests to plantations can lead to a substantial loss of soil organic carbon (SOC) and threaten the function of soil microbial communities. However, the effect of forest conversion on the relationship between SOC dynamics and shifts in the composition and function of the microbial community remain poorly understood, although such information is critical for predicting the long-term effect of land-use change on global SOC dynamics and storage. To fill this knowledge gap, our study investigated the influence of two decades of converting natural forest to a tea plantation on SOC storage, microbial community composition, C-degrading enzyme activity and the abundance of C-degrading microbial functional genes in a subtropical region in China. Two decades after converting natural forest to tea plantation, SOC storage decreased by 30%, and labile SOC [including easily mineralized C (C min), readily oxidizable C (ROC), dissolved organic C (DOC), and microbial biomass C (MBC)] decreased by 21–71%. Land-use change decreased the relative abundance of copiotrophic bacteria but increased the relative abundance of oligotrophic bacteria and lignin-degrading fungi. This resulted in a decrease in cellulase activity and cbh I gene abundance involved in cellulose degradation and conversely an increase in ligninase activity involved in lignin degradation after the conversion. Such shifts in microbial community composition and function associated with land-use change were mediated by the decrease in the labile C pool rather than the total SOC. Our study provided new insights into not only shifts in microbial communities but also specific C functional genes and related enzymes after forest conversion. These findings highlight that labile SOC, as an important functional soil component for microbial communities, significantly affects SOC dynamics and storage. [ABSTRACT FROM AUTHOR]

• This was the first study that was conducted by UHPLC-Quadrupole-Orbitrap-HRMS and HPLC-tri-quadrupole-LIT-MS for Meiguihua oral solution (MOS). • Totally 46 compounds (21 flavonoids and 25 tannins) were firstly detected in MOS, and among them 14 polyphenols were identified for the first time in this herb. • This study provides the simultaneous quantification method for ten compounds including hyperoside and isoquercitrin (isomers). Meiguihua oral solution (MOS), a classical Chinese medicinal formula, was approved by the China Food and Drug Administration for production. However, the quality evaluation of MOS has not been reported. In this present study, qualitative and quantitative analysis of MOS were conducted by ultra high performance liquid chromatography coupled to quadrupole-orbitrap high resolution mass spectrometry (UHPLC-Q-orbitrap-HRMS) and high performance liquid chromatography-tri-quadrupole linear ion trap mass spectrometry (HPLC-tri-Q-LIT-MS). Totally 46 phenolic compounds (21 flavonoids and 25 tannins) were identified in the MOS, among them 14 polyphenols were not reported in raw plant materials of MOS. The simultaneous quantification of ten compounds including gallic acid, quercetin-3- O -sophoroside, ellagic acid, sophoraflavonoloside, hyperoside, isoquercitrin, avicularin, astragalin, quercitrin and juglanin, which were completed in 16 min in the negative electrospray ionization (ESI) mode under multiple reaction monitoring (MRM) method. Linearity was reached fine determination coefficient (r2 > 0.9995). Precisions, repeatability, stability (inter-day and intra-day), and recovery were validated and the relative standard deviations (RSD) were less than 2.9%, 4.7%, 3.6% and 1.79%, respectively. This result proved the high sensitivity and efficiency of the method. The quantitative and qualitative analysis of MOS would provide the substantial basis for further quality control and medicinal values. [ABSTRACT FROM AUTHOR]