Search

Sediments on continental slopes, which are rich in hydrates and organic matters, may release methane into the overlying seawater and even into the atmosphere. To evaluate the impact of sediment emissions on oceanic methane cycling and greenhouse gas emissions, this study assessed the distribution of methane in surface sediments and water columns and calculated the sea-to-air methane flux in the Dongsha area of the northern South China Sea. Results show that the methane concentrations exhibited large spatio-temporal changes. In May 2019, the methane concentrations and sea-to-air methane flux (4.1 ± 3.7 μmol m−2 d−1) were close to the background values, indicating that in situ aerobic methane production was a weak atmospheric methane source. In contrast, in September 2020, abnormally high methane concentrations up to 26.6 nM were detected in the upper slope with water depths of 600–950 m and to the east of a seamount; the average sea-to-air methane flux increased to 11.3 ± 7.9 μmol m−2 d−1. The consistency of methane distribution throughout the water column and surface sediments, with a generally increasing trend with the increasing depth, indicates that in September 2020, sediment emissions were crucial methane sources in seawater and led to moderate emissions into the atmosphere. The heterogeneities of methane concentrations and sea-to-air methane fluxes were attributed to the uneven distribution of methane in the sediments. Besides, strong internal solitary waves in summer and autumn exacerbates methane release, especially around high terrain areas, such as in continental upper slopes and in the upstream side of seamounts. Our results demonstrate that methane released from the sediments, being perhaps affected by internal solitary waves induced sediments disturbance, substantially impacted the oceanic methane cycling in continental slopes. Thus, this study provides new insights into the methane budget of marginal basins. • Abnormally high methane concentrations were detected in the upper slope and to the east of a seamount. • Sediment emissions were crucial methane sources in seawater in the Dongsha area. • ISWs enhance methane released from sediments into atmosphere near high terrain areas in Autumn. [ABSTRACT FROM AUTHOR]

• Nematodes were confirmed in three instars of intermediate host Haematobia irritans. • Parasite growth rate increased as intermediate host developed through instar stages. • P. skrjabini infection of Haematobia irritans begins in the larval stage. Parabronemosis is a disease that severely threatens camel health, causing huge economic losses to industries involved in camel husbandry. Previous studies have reported that horn flies (Haematobia irritans) act as intermediate hosts of Parabronema skrjabini ; however, the infection and developmental processes of P. skrjabini in horn flies remain unclear. In the present study, the infection rates of P. skrjabini were determined in morphologically and molecularly identified horn flies collected from Bactrian camels (Camelus bactrianus) producing regions in Inner Mongolia, China that have high P. skrjabini infection rates. The horn flies were dissected to obtain the nematode larvae at various instar stages. The P. skrjabini found in the different instar stages of horn fly instars were counted and identified to assess the infection and developmental status. Nematode larvae at different developmental stages were obtained from the horn fly instars for further molecular analysis. Sequencing results confirmed that the nematode larvae were P. skrjabini. Furthermore, we found that the mean growth rate of the nematode larva increased as the horn fly instars develops. The results suggested that P. skrjabini infected the horn flies at the larval instar stage, and that the nematode larvae developed simultaneously with the horn fly instars stages. Our findings provide useful information into the elucidation of P. skrjabini infection and life history by studying horn fly development. [ABSTRACT FROM AUTHOR]