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Porcine circovirus 4 (PCV4) is a recently discovered circovirus that was first reported in 2019 in several pigs with severe clinical disease in Hunan province of China, and also identified in pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV). To further investigate the epidemic profile and genetic characteristics of the two viruses, 150 clinical samples were collected from 9 swine farms in Shaanxi and Henan provinces of China, and a SYBR Green I-based duplex quantitative real-time polymerase chain reaction (qPCR) was developed for detecting PCV4 and PRRSV simultaneously. The results showed the limits of detection were 41.1 copies/μL and 81.5 copies/μL for PCV4 and PRRSV, respectively. The detection rates of PCV4 and PRRSV were 8.00% (12/150) and 12.00% (18/150) respectively, and a case of co-infection with PCV4 and PRRSV was found in the lung tissue of a suckling pig with respiratory symptom. Subsequently, the complete genomic sequences of five PCV4 strains were obtained, of which one PCV4 strain (SX-ZX) was from Shaanxi province, and these strains were 1770 nucleotides in length and had 97.7%−99.4% genomic identity with 59 PCV4 reference strains. The genome characteristic of the SX-ZX strain was evaluated from three aspects, a "stem-loop" structure, ORF1 and ORF2. As essential elements for the replication, the 17-bp iterative sequence was predicted as the stem structure, in which three non-tandem hexamers were found at downstream with H1/H2 (12-CGGCACACTTCGGCAC-27) as the minimal binding site. Three of the five PCV4 strains were clustered into PCV4b, which was composed of Suidae, fox, dairy cow, dog and raccoon dog. Phylogenetic analysis revealed that seven PRRSV strains from the present study were clustered into the PRRSV-2 genotype. Collectively, these data extend our understanding of the genome characteristic of PCV4 as well as the molecular epidemiology and the genetic profile of PCV4 and PRRSV. • A duplex qPCR assay was developed for the simultaneous detection of PCV4 and PRRSV. • Limits of detection were 41.1 copies/μL and 81.5 copies/μL for PCV4 and PRRSV, respectively. • A case of co-infection with PCV4 and PRRSV was found in lung tissue of a suckling pig with respiratory symptom. • The 17-bp iterative sequence was predicted as the stem structure. [ABSTRACT FROM AUTHOR]

Abstract: A cache of shoots, leaves and fruits dated by 14C at 2500 years B.P. were unearthed in the Yanghai Tombs, Turpan District in Xinjiang, China. By comparing the morphological and anatomical characteristics of the plant remains found in the tomb and specimens of modern plants, it is shown that the remains belong to Cannabis. Based on the shamanistic background of the deceased man and ancient customs, it is assumed that the Cannabis was utilized for ritual/medicinal purposes. [Copyright &y& Elsevier]

The bistatic radar system has been one of the effective methods to detect the space debris in low earth orbit (LEO). Tianlai radio array with cylindrical-parabolic antennas is designed for dark energy detection, which has large field of view and high sensitivity, offering a fan-beam during the observation. We propose a bistatic radar system, which consists of Tianlai radio array and an incoherent scattering radar (ISR) assumed as a transmitter in the Qujing city of China, to detect space debris. In this paper, we calculate and analyze the detection capabilities of this system. The results show the bistatic radar system has the potential to detect small space debris of less than 10 cm in LEO. We provide a space debris detection method to obtain the position of the cross-beam satisfying the observation requirement with the TLE data of the space debris. The method can solve the problem of space synchronization between the radio array and ISR. We used the long-short baseline method of the radio array to locate the space target. The relationship among positioning error, the azimuth and the elevation angle are also discussed. [ABSTRACT FROM AUTHOR]

Five new meroterpenoids, chrodrimanins O–S ( 1 – 5 ), as well as a known one ( 6 ), were isolated from the fermentation broth of Penicillium sp. SCS-KFD09 isolated from a marine worm, Sipunculusnudus , from Haikou Bay, China. The structures including the absolute configurations of the new compounds were unambiguously elucidated by spectroscopic data and ECD spectra analysis along with quantum ECD calculations. Among them, compound 1 represents the first example of an unusual trichlorinated meroterpenoid with an unique dichlorine functionality. Compounds 1 and 4 – 6 displayed inhibitory activity of protein tyrosine phosphatase 1B (PTP1B) with IC 50 values of 71.6, 62.5, 63.1, and 39.6 μM, respectively, and showed no apparent activity against three tumor cell lines (A549, HepG2, and Hela) and human umbilical vein endothelial cells (HUVEC) at 10 μM. [ABSTRACT FROM AUTHOR]

Abstract: About 20 million tonnes of sludge (with 80% moisture content) is discharged by the sewage treatment plants per year in China, which, if not treated properly, can be a significant source of greenhouse gases (GHGs) emissions. Anaerobic digestion is a conventional sewage sludge treatment method and will continue to be one of the main technologies in the following years. This research has taken into consideration GHGs emissions from typical processes of sludge thickening+anaerobic digestion+dewatering+residue land application in China. Fossil CO2, biogenic CO2, CH4, and avoided CO2 as the main objects is discussed respectively. The results show that the total CO2-eq is about 1133kg/t DM (including the biogenic CO2), while the net CO2-eq is about 372kg/t DM (excluding the biogenic CO2). An anaerobic digestion unit as the main GHGs emission source occupies more than 91% CO2-eq of the whole process. The use of biogas is important for achieving carbon dioxide emission reductions, which could reach about 24% of the total CO2-eq reduction. [Copyright &y& Elsevier]

Two new norlignan derivatives, crassifoside I ( 1 ) and sinensigenin C ( 2 ), were isolated from the rhizomes of Curculigo capitulate, along with six known norlignan derivatives, 1,1-bis(3,4-dihydroxyphenyl)-1-(2-furan)-methane ( 3 ), crassifogenin B ( 4 ), crassifoside A ( 5 ), breviscaside A ( 6 ), crassifoside D ( 7 ), and curcapital ( 8 ). Their structures were elucidated on the basis of spectral evidence and comparisons with literature data. The 1H and 13C NMR data of compound 3 was first assigned. Compounds 3 – 7 were isolated from this plant for the first time. [Copyright &y& Elsevier]