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Your search keyword '"Alajmi, Mohamed Fahad"' showing total 5 results
Start Over Author "Alajmi, Mohamed Fahad" Publisher elsevier b.v.
5 results on '"Alajmi, Mohamed Fahad"'

4. Microwave-assisted polycrystalline Ag/AgO/AgCl nanocomposites synthesis using banana corm (rhizome of Musa sp.) extract: Characterization and antimicrobial studies.

Author

Jaiswal, Krishna Kumar, Banerjee, Ishita, Dutta, Swapnamoy, Verma, Ravikant, Gunti, Lokanadhan, Awasthi, Sharad, Bhushan, Mayank, Kumar, Vinod, Alajmi, Mohamed Fahad, and Hussain, Afzal

Subjects
CHEMICAL fingerprinting, VIBRATIONAL spectra, NANOCOMPOSITE materials, BANANAS, STAPHYLOCOCCUS aureus, ESCHERICHIA coli
Abstract

[Display omitted] • Microwave-irradiated bio-fabrication of Ag-NCs polycrystalline phases using BCE. • Formation of polycrystalline Ag/AgO/AgCl with a maximum crystallite size ∼37 nm. • SEM and HR-TEM micrograph revealed a spherical/oval shape with a size of ∼50 nm. • SERS and SAED pattern confirmed polycrystalline Ag-NCs according to XRD. • Zone of inhibition and MIC showed adequate antibacterial and antifungal properties. The rapid emergence of multidrug-resistant microbes has turned into the main threat to public health worldwide. The preparation and application of nanomaterial-based antimicrobials have increased to address this problem with great significance. This study has investigated the bio-fabrication of polycrystalline Ag-NCs using BCE as a reducer and stabilizer under microwave irradiation. Ag-NCs formations were optimized using varying concentrations of BCE and silver acetate. The XRD confirmed the incidence of phases of nanostructured crystals of Ag/AgO/AgCl. The average crystallite sizes were calculated to be 22.19, 36.65, and 36.65 nm for Ag, AgO, and AgCl, respectively. SERS spectra revealed the vibrational modes of the Ag and Ag-O stretch bands. FTIR spectra have indicated the incidence of chemical fingerprints for Ag-NCs and BCE biomolecules. SEM and HR-TEM micrographs exhibited the almost spherical to the oval shape of ∼50 nm. SAED pattern attested to silver crystallinity in agreement with XRD analysis. The MIC values of the Ag-NCs against Escherichia coli , Staphylococcus aureus , Aspergillus brasiliensis , and Rhizopus stolonifer were observed to be 120, 60, 200, and 100 µg/ml, respectively. This study has demonstrated the facile method to prepare polycrystalline Ag-NCs with appropriate structural and morphological characteristics for antimicrobial applications. [ABSTRACT FROM AUTHOR]

Published
2022
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5. Development and validation of UPLC-PDA method for concurrent analysis of bergenin and menisdaurin in aerial parts of Flueggea virosa (Roxb. ex Willd.).

Author

Hussain, Afzal, Alam, Perwez, Siddiqui, Nasir Ali, Alajmi, Mohamed Fahad, Rehman, Md Tabish, Kalam, Mohd Abul, and Al-Rehaily, Adnan Jathlan

Abstract

Abstract Bergenin and menisdaurin are biologically active components which are found in plant Flueggea virosa (Phyllanthaceae). Bergenin has pharmacological actions such as chemopreventive and antihepatotoxic while menisdaurin has an anti-viral activity which needs its evaluation by an analytical method (UPLC-PDA method) that can be applied to the quality control of pharmaceutical preparations. The developed UPLC-PDA method was applied for identification and quantification of standards bergenin and menisdaurin in the methanol extract of F. virosa (FVME). The analysis was carried out using Eclipse C18 (4.6 × 100 mm, 3.5 µm) UPLC column. The optimized chromatographic condition was achieved at 0.16 mL/min flow rate using gradient system with acetonitrile and water as mobile phase. Both biomarkers were measured at λ max 235 nm in PDA detector at ambient temperature. The developed method furnished sharp and intense peaks of menisdaurin and bergenin at Rt = 2.723 and 3.068 min, respectively along with r2 > 0.99 for both. The recoveries of bergenin and menisdaurin were found in the range of 99.37–101.49% and 98.20–100.08%, respectively. With other validation data, including precision, specificity, accuracy, and robustness, this method demonstrated excellent reliability and sensitivity. The separation parameters i.e. retention, separation, and resolution factors for resolved standards (bergenin and menisdaurin) were >1, which showed good separation. The quantity of bergenin and menisdaurin in the FVME sample was found as 15.16 and 3.28% w/w, respectively. The developed UPLC-PDA method could be conveniently adopted for the routine quality control analysis. [ABSTRACT FROM AUTHOR]

Published
2018
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