1. Association of CD30 transcripts with Th1 responses and proinflammatory cytokines in patients with end-stage renal disease.
- Author
-
Velásquez, Sonia Y., Opelz, Gerhard, Rojas, Mauricio, Süsal, Caner, and Alvarez, Cristiam M.
- Subjects
- *
CD30 antigen , *TH1 cells , *CYTOKINES , *KIDNEY diseases , *SERUM , *PELVIC inflammatory disease , *PATIENTS - Abstract
High serum sCD30 levels are associated with inflammatory disorders and poor outcome in renal transplantation. The contribution to these phenomena of transcripts and proteins related to CD30-activation and -cleavage is unknown. We assessed in peripheral blood of end-stage renal disease patients (ESRDP) transcripts of CD30-activation proteins CD30 and CD30L, CD30-cleavage proteins ADAM10 and ADAM17, and Th1- and Th2-type immunity-related factors t-bet and GATA3. Additionally, we evaluated the same transcripts and release of sCD30 and 32 cytokines after allogeneic and polyclonal T-cell activation. In peripheral blood, ESRDP showed increased levels of t-bet and GATA3 transcripts compared to healthy controls (HC) (both P < 0.01) whereas levels of CD30 , CD30L , ADAM10 and ADAM17 transcripts were similar. Polyclonal and allogeneic stimulation induced higher levels of CD30 transcripts in ESRDP than in HC (both P < 0.001). Principal component analysis (PCA) in allogeneic cultures of ESRDP identified two correlation clusters, one consisting of sCD30, the Th-1 cytokine IFN-γ, MIP-1α, RANTES, sIL-2Rα, MIP-1β, TNF-β, MDC, GM-CSF and IL-5, and another one consisting of CD30 and t-bet transcripts, IL-13 and proinflammatory proteins IP-10, IL-8, IL-1Rα and MCP-1. Reflecting an activated immune state, ESRDP exhibited after allostimulation upregulation of CD30 transcripts in T cells, which was associated with Th1 and proinflammatory responses. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF