12 results on '"Andrade, Sérgio Faloni de"'
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2. Diuretic and natriuretic effect of luteolin in normotensive and hypertensive rats: Role of muscarinic acetylcholine receptors
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Boeing, Thaise, da Silva, Luísa Mota, Mariott, Marihá, Andrade, Sérgio Faloni de, and de Souza, Priscila
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- 2017
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3. Artepillin C, drupanin, aromadendrin-4′-O-methyl-ether and kaempferide from Brazilian green propolis promote gastroprotective action by diversified mode of action.
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Costa, Philipe, Almeida, Marilia O., Lemos, Marivane, Arruda, Caroline, Casoti, Rosana, Somensi, Lincon Bordignon, Boeing, Thaise, Mariott, Marihá, da Silva, Rita de Cássia Melo Vilhena de Andrade Fonseca, Stein, Bruna De Paoli, Souza, Priscila de, dos Santos, Ana Caroline, Bastos, Jairo K., da Silva, Luísa Mota, and Andrade, Sérgio Faloni de
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PEPTIC ulcer prevention , *GASTRITIS , *ANIMAL experimentation , *ETHANOL , *FLAVONOIDS , *FLUIDS , *GASTRIC juice , *GLYCOPROTEINS , *HISTOLOGICAL techniques , *HYDROCHLORIC acid , *HYDROGEN-ion concentration , *INDOMETHACIN , *INFLAMMATORY mediators , *INTRAPERITONEAL injections , *MASS spectrometry , *MICE , *NUCLEAR magnetic resonance spectroscopy , *ORAL drug administration , *OXIDOREDUCTASES , *PEPSIN , *PROPOLIS , *RATS , *STOMACH , *SUPEROXIDE dismutase , *TRANSFERASES , *TUMOR necrosis factors , *OXIDATIVE stress , *PHARMACODYNAMICS , *PREVENTION - Abstract
Ethopharmacological relevance The propolis is extensively used in folk medicine in natura or to prepare pharmaceutical formulations since ancient time to improve health or prevent diseases, among them gastrointestinal disorders. Aiming to contribute in the scientific validation about the popular use of Brazilian Green propolis (BGP) against gastritis and gastric ulcer, this work evaluated the antiulcer potential of isolated compounds from BGP, three prenylated p -coumaric acid derivatives and two flavonoids, respectively named: 3,5 diprenyl-4-hydroxycinnamic acid (artepillin C) ( 1 ), 3-prenyl-4-dihydroxycinnamoiloxy cinnamic acid (baccharin) ( 2 ), 3-prenyl-4-hydroxycinnamic acid (drupanin) ( 3 ), aromadendrin-4′-O-methyl-ether ( 4 ) and kaempferide ( 5 ). Material and methods The compounds were characterized by nuclear magnetic resonance and mass spectrometry. Their gastroprotective effects were evaluated against ethanol/HCl- and indomethacin-induced ulcer in mice. Further, histological, histochemical, oxidative and inflammatory parameters were analyzed at ulcerated tissue. Acid antisecretory activities also were also assessed. Results Compound 2 did not reduce the ethanol/HCl- induced ulcer at 30 mg/kg (p.o), whereas the minimum oral gastroprotective doses of 1 , 3 , 4 and 5 were 0.3, 0.3, 3 and 3 mg/kg, respectively. Besides, these compounds prevented ethanol/HCl-induced ulcer by intraperitoneal route, as well as indomethacin-induced ulcer by oral route. The gastroprotection was accompanied by normalization of superoxide dismutase, catalase and glutathione- S -transferase activities and reduction in myeloperoxidase activity. Moreover, the compounds 4 and 5 increased the gastric mucin content and 1 reduced TNF amount. Furthermore, 1 , 3 , 4 and 5 decreased volume, pH, total acidity and pepsin activity of the gastric juice from rats. Conclusions Together, our findings showed a diversified mode of action elicited by 1 , 3 , 4 and 5 on the gastroprotection and contribute to explain the anti-ulcer activity reported for BGP. [ABSTRACT FROM AUTHOR]
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- 2018
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4. Preclinical evaluation of the diuretic and saluretic effects of (-)-epicatechin and the result of its combination with standard diuretics.
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Mariano, Luísa Nathália Bolda, Boeing, Thaise, da Silva, Rita de Cássia Melo Vilhena de Andrade Fonseca, Cechinel-Filho, Valdir, Niero, Rivaldo, da Silva, Luisa Mota, de Souza, Priscila, and Andrade, Sérgio Faloni de
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DIURETICS , *EPICATECHIN , *FLAVONOIDS , *FLAVONOLS , *PROSTANOIDS , *HIGH performance liquid chromatography - Abstract
Graphical abstract Highlights • This study shows the diuretic and saluretic actions of (-)-epicatechin (EPI) in rats. • The combination with HCTZ strengthened EPI-induced diuresis. • The treatment with amiloride plus EPI preserved the potassium-sparing effect. • EPI-induced diuresis was precluded in the presence of a cyclooxygenase inhibitor. Abstract Several studies have suggested that (-)-epicatechin-containing foods and plant extracts benefit conditions that affect the cardiovascular system, such as hypertension and endothelial dysfunction. However, no study was conducted so far to evaluate the potential of this flavonoid on diuretic activity assay. For that, female Wistar normotensive (NTR) and spontaneously hypertensive rats (SHR) received a single oral treatment with (-)-epicatechin (EPI), hydrochlorothiazide (HCTZ) or just vehicle (VEH). The effects of EPI in combination with diuretics for clinical use, as well as with L-NAME, atropine and indomethacin were also explored. Cumulative urine volume, plasma and urinary parameters were evaluated at the end of 8 h experiment. When given to NTR and SHR, at doses of 0.3, 1 and 3 mg/kg, EPI was able to stimulate both diuresis and saluresis (Na+, K+ and Cl−), without interfering with plasma electrolyte content or urinary pH and uric acid values, when compared with VEH-treated only rats. The combination with HCTZ, but not with furosemide or amiloride, successfully strengthened EPI-induced diuresis. This effect was not accompanied by a potentiation of the saluretic effects. On the other hand, when given EPI in combination with amiloride, a significant increase in Cl− excretion and maintenance of the potassium-sparing effects characteristic of this class of diuretics were detected. In addition, the diuretic effect of EPI was enhanced after pretreatment with L-NAME and its action was significantly precluded in the presence of indomethacin, a cyclooxygenase inhibitor. In conclusion, this study shows the diuretic and saluretic properties of EPI in rats, adding another biological activity whose effect may contribute to the different positive actions already described. [ABSTRACT FROM AUTHOR]
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- 2018
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5. Essential oil of Cymbopogon citratus (lemongrass) and geraniol, but not citral, promote gastric healing activity in mice.
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Venzon, Larissa, Mariano, Luísa Nathália Bolda, Somensi, Lincon Bordignon, Boeing, Thaise, de Souza, Priscila, Wagner, Theodoro Marcel, Andrade, Sérgio Faloni de, Nesello, Luciane Angela Nottar, and da Silva, Luísa Mota
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LEMONGRASS , *ESSENTIAL oils , *GASTRIC diseases , *HISTOCHEMISTRY , *MUCINS - Abstract
Cymbopogon citratus , popularly known as lemongrass, is used for the treatment of gastric, nervous and hypertensive disorders, in addition to its use in the food and pharmaceutical industries. This study evaluated the gastroprotective and gastric healing effect of essential oil of C. citratus (EOCC), citral and geraniol at doses of 1–100 mg/kg (p.o) on acute ethanol-induced ulcer and chronic acetic acid-induced ulcer. Histological and histochemical evaluation was also performed, as well as the in vitro evaluation of the effects of these phytochemicals on H + /K + -ATPase activity. In the ethanol-induced gastric ulcer, the minimum effective oral dose of EOCC, citral and geraniol were 10, 100 and 3 mg/kg, reducing the ulcer area by 51.67%, 96.57% and 55.74%, respectively, compared to vehicle group (25.82 ± 3.59 mm 2 ). Moreover, EOCC (10 mg/kg, p.o) and geraniol (3 mg/kg), but not citral (100 mg/kg), accelerated the gastric healing process by 34.52 and 80.57%, compared to acetic-acid ulcerated group treated with vehicle (36.04 ± 1.03 mm 2 ). These healing effects were confirmed histologically by the contraction of the ulcer base and by the enhancement on mucin staining in slices of ulcer site from mice treated with EOCC or geraniol. Interestingly, EOCC and citral at 100 μg/ml inhibited the H + / K + -ATPase activity by 28.26% and 44.36%, whereas geraniol did not change this parameter. Together, these findings confirm the gastroprotective and healing gastric ulcer effects of essential oil from aerial parts of C. citratus and added the information that geraniol, but not citral, promotes healing effects on installed ulcers. [ABSTRACT FROM AUTHOR]
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- 2018
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6. Prolonged diuretic and saluretic effect of nothofagin isolated from Leandra dasytricha (A. Gray) Cogn. leaves in normotensive and hypertensive rats: Role of antioxidant system and renal protection.
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de Almeida, Camila Leandra Bueno, Cechinel-Filho, Valdir, Boeing, Thaise, Mariano, Luísa Nathália Bolda, Silva, Luísa Mota da, Andrade, Sérgio Faloni de, and de Souza, Priscila
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HYDROCHLOROTHIAZIDE , *LABORATORY rats , *DIURETICS , *SUPEROXIDE dismutase , *GLUTATHIONE - Abstract
Although the acute diuretic effect of nothofagin has been recently demonstrated, its effects after dose-repeated treatment have not yet been explored. For that, male Wistar normotensive (NTR) and spontaneously hypertensive rats (SHR) were orally treated, once a day, with vehicle (VEH: distilled water; 1 ml/kg), hydrochlorothiazide (HCTZ; 10 mg/kg) or nothofagin (NOT; 1 mg/kg). The cumulative diuretic index and urinary electrolytes excretion were measured each 24 h. On the last day of the experiment (7th day), urine, blood and kidney samples were collected for biochemical and molecular analyzes. The urinary volume of both NTR and SHR were significantly increased with the treatment with NOT (from the second to the seventh day of treatment), with final values reaching an increase of 56% and 82%, respectively, when compared with VEH-treated group. This effect was associated with increased levels of urinary excretion of Na + and Cl − , without any changes on K + excretion. None of the treatments modified urinary pH or density values. Importantly, neither the NOT nor the HCTZ caused any change in body weight following the dose-repeated treatment, and also did not provoke an electrolytic disturbance. Regarding the renal analyzes, when compared with the vehicle-treated NTR group, the activity of superoxide dismutase (SOD) and the reduced glutathione (GSH) levels in kidney homogenates of the SHR group were decreased, while the generation of lipid hydroperoxides were significantly increased. The daily treatment with NOT was able to restore the GSH levels and SOD activity, as well as reduced the lipoperoxidation in the kidney homogenates obtained from SHR animals. Finally, NOT significantly augmented the levels of nitrite, a marker of nitric oxide production, in the plasma obtained from SHR group when compared with the vehicle-treated only NTR. This study revealed the prolonged diuretic and saluretic effect of nothofagin in normotensive and hypertensive rats. Our data also showed the renal protective effects of nothofagin by the improvement of antioxidative capacity, as well as by the augmented bioavailability of plasma nitric oxide in the hypertensive group. [ABSTRACT FROM AUTHOR]
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- 2018
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7. Diuretic, natriuretic and potassium-sparing effect of nothofagin isolated from Leandra dasytricha (A. Gray) Cogn. leaves in normotensive and hypertensive rats.
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de Almeida, Camila Leandra Bueno, Boeing, Thaise, Somensi, Lincon Bordignon, Steimbach, Viviane Miranda Bispo, da Silva, Luísa Mota, Andrade, Sérgio Faloni de, Delle Monache, Franco, Cechinel-Filho, Valdir, and de Souza, Priscila
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POTASSIUM-sparing diuretics , *ANIMAL models in research , *HYPERTENSION , *CARDIOVASCULAR disease treatment , *DIHYDROCHALCONES , *ETHYL acetate - Abstract
Active constituents from natural origin have long been used for the treatment of patients suffering from cardiovascular and renal diseases. This study therefore aimed to investigate the diuretic and natriuretic properties of nothofagin, a dihydrochalcone isolated from Leandra dasytricha (A. Gray) Cogn. leaves in normotensive and hypertensive rats. Male Wistar normotensive rats were orally treated with vehicle (1 ml/kg); hydrochlorothiazide (HCTZ; 25 mg/kg); ethyl acetate fraction from L. dasytricha (EALD; 3–30 mg/kg) and nothofagin (NOT; 0.3–3 mg/kg). Spontaneously hypertensive rats (SHR) received NOT (1 mg/kg), HCTZ (25 mg/kg) or vehicle. The cumulative diuretic index, urinary electrolytes excretion (Na + and K + ), pH, density and conductivity were measured at the end of the experiment (after 8 h). A7r5 and L929 cell lines were used to measure cell viability after exposure to NOT. Nitric oxide generation was quantified in A7r5 cell supernatant, and DPPH assay was used for evaluating the antioxidant properties of NOT. The urinary volume of normotensive rats were increased after the treatment with EALD, without any changes in Na + or K + excretion. NOT was able to induce diuresis and natriuresis, but not kaliuresis, in both normotensive and hypertensive rats. The reduction in prostanoids generation through cyclooxygenase inhibition, as well as the muscarinic receptor antagonism, fully avoided NOT-induced increases in diuretic index. NOT, which did not interfere with L929 or A7r5 cell viability, was able to stimulate nitric oxide generation in A7r5 cell, besides showing an antioxidant effect in scavenging the free-radical DPPH. Taken together, our study shows, for the first time, the diuretic, natriuretic and potassium-sparing effect of nothofagin in rats, which was associated with prostanoids generation, muscarinic receptor activation and antioxidant properties. [ABSTRACT FROM AUTHOR]
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- 2017
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8. In vivo evaluation of the genetic toxicity of Rubus niveus Thunb. (Rosaceae) extract and initial screening of its potential chemoprevention against doxorubicin-induced DNA damage.
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Tolentino, Flora, Araújo, Priscila Alves de, Marques, Eduardo de Souza, Petreanu, Marcel, Andrade, Sérgio Faloni de, Niero, Rivaldo, Perazzo, Fábio F., Rosa, Paulo César Pires, and Maistro, Edson Luis
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ALTERNATIVE medicine , *ANIMAL experimentation , *BIOLOGICAL assay , *BIOPHYSICS , *BONE marrow , *CHEMOPREVENTION , *CHROMOSOMES , *COMBINED modality therapy , *DNA , *DOXORUBICIN , *DRUG toxicity , *RESEARCH methodology , *MEDICINAL plants , *MUTAGENS , *PROBABILITY theory , *PHYTOCHEMICALS , *PLANT extracts , *PLANT anatomy , *DESCRIPTIVE statistics - Abstract
Ethnopharmacological relevance Rubus niveus Thunb. plant belongs to Rosaceae family and have been used traditionally to treat wounds, burns, inflammation, dysentery, diarrhea and for curing excessive bleeding during menstrual cycle. The present study was undertaken to investigate the in vivo genotoxicity of Rubus niveus aerial parts extract and its possible chemoprotection on doxorubicin (DXR)-induced DNA damage. In parallel, the main phytochemicals constituents in the extract were determined. Materials and methods The animals were exposed to the extract for 24 and 48 h, and the doses selected were 500, 1000 and 2000 mg/kg b.w. administered by gavage alone or prior to DXR (30 mg/kg b.w.) administered by intraperitoneal injection. The endpoints analyzed were DNA damage in bone marrow and peripheral blood cells assessed by the alkaline alkaline (pH>13) comet assay and bone marrow micronucleus test. Results and conclusion The results of chemical analysis of the extract showed the presence of tormentic acid, stigmasterol, quercitinglucoronide (miquelianin) and niga-ichigoside F1 as main compounds. Both cytogenetic endpoints analyzed showed that there were no statistically significant differences ( p >0.05) between the negative control and the treated groups with the two higher doses of Rubus niveus extract alone, demonstrating absence of genotoxic and mutagenic effects. Aneugenic/clastogenic effect was observed only at 2000 mg/kg dose. On the other hand, in the both assays and all tested doses were observed a significant reduction of DNA damage and chromosomal aberrations in all groups co-treated with DXR and extract compared to those which received only DXR. These results indicate that Rubus niveus aerial parts extract did not revealed any genotoxic effect, but presented some aneugenic/clastogenic effect at higher dose; and suggest that it could be a potential adjuvant against development of second malignant neoplasms caused by the cancer chemotherapic DXR. [ABSTRACT FROM AUTHOR]
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- 2015
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9. Dragon's blood Croton palanostigma induces genotoxic effects in mice.
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Maistro, Edson Luis, Ganthous, Giulia, Machado, Marina da Silva, Zermiani, Tailyn, Andrade, Sérgio Faloni de, Rosa, Paulo Cesar Pires, and Perazzo, Fabio Ferreira
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DNA analysis , *LIVER analysis , *MEDICINAL plants , *ALTERNATIVE medicine , *ANALYSIS of variance , *ANIMAL experimentation , *BIOLOGICAL assay , *BIOPHYSICS , *HIGH performance liquid chromatography , *LEUCOCYTES , *RESEARCH methodology , *MICE , *MUTAGENS , *ORAL drug administration , *RESEARCH funding , *STATISTICS , *PHYTOCHEMICALS , *PLANT extracts , *DATA analysis , *DATA analysis software , *DESCRIPTIVE statistics - Abstract
Abstract: Ethnopharmacological relevance: Dragon's blood is a dark-red sap produced by species from the genus Croton (Euphorbiaceae), which has been used as a famous traditional medicine since ancient times in many countries, with scarce data about its safe use in humans. In this research, we studied genotoxicity and clastogenicity of Croton palanostigma sap using the comet assay and micronucleus test in cells of mice submitted to acute treatment. Material and methods: HPLC analysis was performed to identify the main components of the sap. The sap was administered by oral gavage at doses of 300mg/kg, 1000mg/kg and 2000mg/kg. For the analysis, the comet assay was performed on the leukocytes and liver cells collected 24h after treatment, and the micronucleus test (MN) on bone marrow cells. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio). Results and conclusion: The alkaloid taspine was the main compound indentified in the crude sap of Croton palanostigma. The results of the genotoxicity assessment show that all sap doses tested produced genotoxic effects in leukocytes and liver cells and also produced clastogenic/aneugenic effects in bone marrow cells of mice at the two higher doses tested. The PCE/NCE ratio indicated no cytotoxicity. The data obtained suggest caution in the use of Croton palanostigma sap by humans considering its risk of carcinogenesis. [Copyright &y& Elsevier]
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- 2013
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10. Genotoxicity assessment of Garcinia achachairu Rusby (Clusiaceae) extract in mammalian cells in vivo
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Marques, Eduardo de Souza, Silva, Suellen, Niero, Rivaldo, Andrade, Sérgio Faloni de, Rosa, Paulo Cesar Pires, Perazzo, Fabio Ferreira, and Maistro, Edson Luis
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LIVER analysis , *ALTERNATIVE medicine , *ANIMAL experimentation , *BIOLOGICAL assay , *BIOLOGICAL models , *BIOPHYSICS , *BONE marrow , *DRUG toxicity , *GENES , *HISTOLOGICAL techniques , *LEUCOCYTES , *RESEARCH methodology , *MEDICINAL plants , *MICE , *ORAL drug administration , *SEEDS , *TESTIS , *PLANT extracts , *DESCRIPTIVE statistics - Abstract
Abstract: Ethnopharmacological relevance: Garcinia achachairu Rusby (Clusiaceae) is popularly known as “achachairu”, and is used in Bolivian folk medicine for its healing, digestive, and laxative properties, and in the treatment of gastritis, rheumatism and inflammation. Despite its widespread therapeutic use, there is a lack of data regarding its in vivo genotoxic effects. Therefore, in this study, we used the comet assay and the micronucleus test, respectively, to evaluate the possible genotoxic and clastogenic effects of Garcinia achachairu seed extract (GAE) on different cells of mice. Material and methods: The GAE was administered by oral gavage at doses of 500, 1000 and 2000mg/kg. For the analysis, the comet assay was performed on the leukocytes (collected 4 and 24h after treatment), liver, bone marrow and testicular cells (collected 24h after treatment), and the micronucleus test (MN) on bone marrow cells. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio). Results and conclusion: The results showed that GAE did not induce significant DNA damage in leukocytes (4h and 24h samples), liver, bone marrow and testicular cells (24h samples). GAE also did not show any significant increase in micronucleated polychromatic erythrocytes (MNPCEs) at the three tested doses. The PCE/NCE ratio indicated no cytotoxicity. Under our experimental conditions, the data obtained suggest that a single oral administration of G. achachairu extract does not cause genotoxicity and clastogenicity in different cells of mice. [Copyright &y& Elsevier]
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- 2012
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11. Gastroprotective activity of hydroalcoholic extract obtained from the leaves of Brassica oleracea var. acephala DC in different animal models
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Lemos, Marivane, Santin, José Roberto, Júnior, Luiz Carlos Klein, Niero, Rivaldo, and Andrade, Sérgio Faloni de
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PEPTIC ulcer prevention , *ACID-base equilibrium , *ALTERNATIVE medicine , *ANALYSIS of variance , *ANIMAL experimentation , *ANTIULCER drugs , *BIOPHYSICS , *DOSE-effect relationship in pharmacology , *LEAVES , *RESEARCH methodology , *MEDICINAL plants , *MUCUS , *PLANT extracts , *PHARMACODYNAMICS - Abstract
Abstract: Ethnopharmacological relevance: Brassica oleracea var. acephala DC has been extensively used in Brazilian traditional medicine to treat gastric ulcer. Aim of the study: This study was conducted to evaluate the antiulcerogenic property of hydroalcoholic extract obtained from the leaves of Brassica oleracea. Materials and methods: Antiulcer assays were performed using the protocol of ulcer induced by ethanol/HCl, and non-steroidal anti-inflammatory drugs (NSAIDs). Parameters of gastric secretion (volume, pH and [H+]) were determined by the pylorus ligation model and mucus in gastric contents. Results: In the ethanol-induced ulcer model, we observed a significant reduction in all the parameters analyzed, obtaining curative ratios of 58.8±11.5, 86.2±12.2 and 42.8±6.6% for the groups treated with 50 and 100mg/kg of extract and omeprazole (30mg/kg), respectively. The dose of 25mg/kg of hydroalcoholic extract of Brassica oleracea showed no significant results. In the indomethacin-induced ulcer, the percentages of ulcer inhibition were 64.3±9.9, 66.4±12.3 and 81.2±7.5% for the groups treated with 50 and 100mg/kg extract and positive control (cimetidine, 100mg/kg), respectively. The results showed a significant increase in pH and mucus production in the groups treated with Brassica oleracea when compared with the control group. No sign of toxicity was observed in the acute toxicity study. Conclusions: The results of the present study show that hydroalcoholic extract of Brassica oleracea displays antiulcer activity, as demonstrated by the significant inhibition of ulcer formation induced using different models. The data suggest that the effectiveness of the extract is based on its ability to stimulate the synthesis of mucus, increase pH and decrease H+ ions in the stomach. This work corroborates the ethnopharmacology use of Brassica oleracea preparations, contributing to its pharmacological validation by suggesting that preparations obtained from Brassica oleracea could be used for the development of new phytopharmaceuticals for the treatment of gastric ulcer. [Copyright &y& Elsevier]
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- 2011
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12. Evaluation of antiulcer activity of the main phenolic acids found in Brazilian Green Propolis
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Barros, Muriel Primon de, Lemos, Marivane, Maistro, Edson Luis, Leite, Mateus Freire, Sousa, João Paulo Barreto, Bastos, Jairo Kennup, and Andrade, Sérgio Faloni de
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PHENOLIC acids , *PROPOLIS , *ANTIULCER drugs , *NONSTEROIDAL anti-inflammatory agents , *HYDROGEN-ion concentration , *PRECANCEROUS conditions , *CIMETIDINE , *OMEPRAZOLE , *THERAPEUTICS - Abstract
Abstract: Aim of the study: In a previous study, our group described the gastric protective effect of the hydroalcoholic extract of Brazilian green propolis. The main compounds found in Brazilian green propolis include phenolic acids, such as: caffeic, ferulic, p-coumaric and cinnamic acids. This study was therefore carried out to evaluate the antiulcerogenic property of the main phenolic acids found in Brazilian Green Propolis. Material and methods: The anti-ulcer assays were performed using the following protocols: nonsteroidal-antiinflammatory drug (NSAID)-induced ulcer, ethanol-induced ulcer, and stress-induced ulcer. The effects of the phenolic acids on gastric content volume, pH and total acidity, using the pylorus ligated model, were also evaluated. Results: It was observed that treatment using doses of 50 and 250mg/kg of caffeic, ferulic, p-coumaric and cinnamic acids and positive controls (omeprazol or cimetidine) significantly diminished the lesion index, the total area of the lesion and the percentage of lesion in comparison with the negative control groups. In addition, the percentage of ulcer inhibition was significantly higher in the groups treated with the different phenolic acids, cimetidine or omeprazol, in all the protocols used, compared with the negative control groups. In the model to determine gastric secretion, using ligated pylorus, treatment with phenolic acids and cimetidine reduced the volume of gastric juice and total acidity and significantly increased the gastric pH (p <0.05), compared with the control group, with the exception of the group treated with 50mg/kg of p-coumaric acid, in which no significant difference was observed, compared with the control. In relation to the acute toxicity, none sign of toxicity was observed when phenolic acids, used in this study, were administered for rats in dose of 2000mg/kg. Conclusions: In conclusion, the results of this study show that caffeic, ferulic, p-coumaric and cinnamic acids display antiulcer activity. [Copyright &y& Elsevier]
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- 2008
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