1. A multiple aptasensor for ultrasensitive detection of miRNAs by using covalent-organic framework nanowire as platform and shell-encoded gold nanoparticles as signal labels.
- Author
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Cao, Zhengming, Duan, Fenghe, Huang, Xiaoyu, Liu, Yang, Zhou, Nan, Xia, Lei, Zhang, Zhihong, and Du, Miao
- Subjects
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GOLD nanoparticles , *NANOWIRES , *POLYCONDENSATION , *MICRORNA , *SEMICONDUCTOR nanowires , *LABELS , *SINGLE-stranded DNA , *DETECTION limit - Abstract
We report herein a novel multiple electrochemical aptasensor based on covalent–organic framework (COF) for sensitive and simultaneous detection of miRNA 155 and miRNA 122, by using shell-encoded gold nanoparticles (Au NPs) as signal labels (AgNCs@AuNPs and Cu 2 O@AuNPs, respectively, NCs = nanoclusters). A new COF nanowire was synthesized via condensation polymerization of 1,3,6,8-tetra(4-carboxylphenyl)pyrene and melamine (represented by TBAPy-MA–COF–COOH) for multiple aptasensor fabrication. The nanowire was then used as a platform for anchoring single-strand DNA (ssDNA), which was hybridized with the complementary aptamer (cApt) probes of miRNA 155 and miRNA 122. AgNCs@AuNPs and Cu 2 O@AuNPs modified with cApts show separated differential pulse voltammetry (DPV) peaks at 0.08 and −0.1 V, respectively. The signal labels immobilized with cApts were released from the hybridized DNA complex and bound to their corresponding targets when contacting miRNAs. This phenomenon results in the substantial decline of the DPV peak current density of the signal labels. The developed TBAPy-MA–COF–COOH-based aptasensor has superior performance for sensing miRNA 155 and miRNA 122 simultaneously, with ultrasensitive low detection limits of 6.7 and 1.5 fM (S/N = 3), respectively, a wide linear range of 0.01–1000 pM, and high selectivity and applicability for serum samples. The proposed TBAPy-MA-based aptasensor demonstrates potential for simultaneous detection of multiple cancer biomarkers by replacing other ssDNA and aptamer strands. A new class multiple electrochemical aptasensor based on a novel covalent organic framework (COF) coupling with the shell-encoded gold nanoparticles (Au NPs) as signal labels was developed for sensitively detecting miRNA 155 and miRNA 122. The construction COF-based aptasensor exhibited low cross-reactivity, high sensitivity, good selectivity, and acceptable applicability in serum samples. Image 1 • TBAPy-MA-COF nanowires synthesized by a condensation polymerization of 1,3,6,8-tetra(4-carboxylphenyl)pyrene and melamine. • TBAPy-MA-COF-based aptasensor for the multiple miRNA 155 and miRNA 122 detection. • Low cross-reactivity, high sensitivity and selectivity, and acceptable recyclability of TBAPy-MA-COF-based aptasensor. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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