6 results on '"Capozza, Paolo"'
Search Results
2. A longitudinal observational study in two cats naturally-infected with hepadnavirus.
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Capozza, Paolo, Lanave, Gianvito, Diakoudi, Georgia, Stasi, Fabio, Ghergo, Paola, Ricci, Dominga, Santo, Giacinto, Arena, Gianluca, Grillo, Isidoro, Delle Donne, Elisabetta, Di Lisio, Francesca, Zini, Eric, Callegari, Carolina, Valente, Luciana, Camero, Michele, Di Martino, Barbara, Beatty, Julia, Barrs, Vanessa R., Buonavoglia, Canio, and Martella, Vito
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ALANINE aminotransferase , *CATS , *HEPATITIS B virus , *LONGITUDINAL method , *SCIENTIFIC observation - Abstract
• A hepatitis B virus (HBV) analogue, DCH, was discovered in 2018 in domestic cats. • It is unclear if DCH may cause liver damage and chronic infections. • Upon re-testing of DCH-positive animals, two cats were persistently viremic. • A 9-year-old male cat was viremic from February 2019 through January 2020. • This pattern of infection mirrors what described with HBV in human patients. Hepatitis B virus (HBV) is a major cause of liver disease in humans including chronic hepatitis and hepatocellular carcinoma. Domestic cat hepadnavirus (DCH), a novel HBV-like hepadnavirus, was identified in domestic cats in 2018. From 6.5 %–10.8 % of pet cats are viremic for DCH and altered serological markers suggestive of liver damage have been identified in 50 % of DCH-infected cats. DCH DNA has been detected in association with characteristic lesions of chronic hepatitis and with hepatocellular carcinoma in cats, suggesting a possible association. In this study longitudinal molecular screening of cats infected with DCH was performed to determine if DCH can cause chronic infections in cats. Upon re-testing of sera from five DCH-positive animals, 2–10 months after the initial diagnosis, three cats tested negative for DCH on two consecutive occasions using quantitative PCR. Two other cats remained DCH-positive, including an 8-month-old female cat re-tested four months after the initial positive result, and a 9-year-old male cat, which tested positive for DCH on six occasions over an 11-month period. The latter had a history of chronic hepatopathy with jaundice, lethargy and elevated serum alanine transaminase levels (ALT). During the period of observation, DCH titers ranged between 1.64 × 105 and 2.09 × 106 DNA copies/mL and ALT was persistently elevated, suggesting chronic infection. DCH DNA was not detected in oral, conjunctival, preputial and rectal swabs from the two animals collected at several time points. Long-term (chronic) infection would be consistent with the relatively high number of viremic cats identified in epidemiological investigations, with the possible association of DCH with chronic hepatic pathologies and with what described with HBV in human patients. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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3. Identification of a novel parvovirus in domestic cats.
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Diakoudi, Georgia, Lanave, Gianvito, Capozza, Paolo, Di Profio, Federica, Melegari, Irene, Di Martino, Barbara, Pennisi, Maria Grazia, Elia, Gabriella, Cavalli, Alessandra, Tempesta, Maria, Camero, Michele, Buonavoglia, Canio, Bányai, Krisztián, and Martella, Vito
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PARVOVIRUS diseases , *CAT diseases , *VIRAL genetics , *RESPIRATORY diseases , *VETERINARY medicine - Abstract
Highlights • A novel protoparvovirus (bufavirus) was identified in cats. • The feline bufavirus was more common in respiratory samples of juvenile cats. • The feline bufavirus was highly similar to a canine bufavirus. • The carnivore bufaviruses were phylogenetically related to primate bufaviruses. • Carnivore bufaviruses are genetically distinct from feline/canine protoparvovirus-1. Abstract A novel protoparvovirus species was identified in domestic cats. The virus was distantly related to the well-known feline (feline panleukopenia virus) and canine (canine parvovirus type 2) parvoviruses, sharing low nucleotide identities in the capsid protein 2 (less than 43%). The virus was genetically similar (100% at the nucleotide level) to a newly identified canine protoparvovirus, genetically related to human bufaviruses. The feline bufavirus appeared as a common element of the feline virome, especially in juvenile cats, with an overall prevalence of 9.2%. The virus was more common in respiratory samples (9.5%–12.2%) than in enteric samples of cats (2.2%). The role of bufaviruses in the etiology of feline respiratory disease complex, either as a primary or a secondary agents, should be defined. [ABSTRACT FROM AUTHOR]
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- 2019
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4. Virucidal activity of ginger essential oil against caprine alphaherpesvirus-1.
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Camero, Michele, Lanave, Gianvito, Catella, Cristiana, Capozza, Paolo, Gentile, Arturo, Fracchiolla, Giuseppe, Britti, Domenico, Martella, Vito, Buonavoglia, Canio, and Tempesta, Maria
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HERPESVIRUS disease treatment , *GINGER , *THERAPEUTIC use of essential oils , *ANTIVIRAL agents , *VETERINARY virology - Abstract
Highlights • EOs function as antibacterial, antiviral, antifungal and insecticidal agents. • GEO proved to inhibit replication of HSV-2 in vitro halting plaque formation by 90%. • Anti-infective activity of GEO against CpHV-1 was assessed in MDBK cells. • GEO was not able to inhibit CpHV-1 adsorption and/or replication. • GEO was able to inactivate cell-free CpHV-1 up to 100%. Abstract The emergence of alphaherpesvirus strains resistant to commonly used antiviral drugs has prompted the research for alternative, biologically active anti-herpetic agents. Essential oils (EOs) have shown anti-infective properties against human herpes simplex viruses (HSV-1 and -2). Caprine alphaherpesvirus 1 (CpHV-1) induces genital lesions in its natural host and it is regarded as a useful homologous animal model for the study of HSV-2 infection, chiefly for the assessment of antiviral drugs in in vivo studies. In the present study we evaluated the activity in vitro of ginger EO (GEO) against CpHV-1. GEO was found to be effective as virucide on cell-free virus, inactivating CpHV-1 up to 100%. The virucidal activity of GEO is likely accounted for by disruption of herpesvirus envelope and its associated structures which are necessary for virus adsorption and entry into host cells. On the opposite, GEO was not able to inhibit virus adsorption and/or replication, as treatment of cells before and after infection did not abolish virus infectivity. GEO could be suggested for topical applications in in vivo experiments using CpHV-1/goat model, since the lipophilic nature of EOs favours their adsorption through the cutaneous/mucosal barrier, either alone or in conjunction with other molecules. These findings open several perspectives in terms of therapeutic possibilities for a number of human and animal alphaherpesviruses. [ABSTRACT FROM AUTHOR]
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- 2019
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5. Kosher slaughter paradigms: Evaluation of slaughterhouse inspection procedures.
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Bozzo, Giancarlo, Di Pinto, Angela, Bonerba, Elisabetta, Ceci, Edmondo, Mottola, Anna, Roma, Rocco, Capozza, Paolo, Samoilis, Giorgio, Tantillo, Giuseppina, and Celano, Gaetano Vitale
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KOSHER salt , *SLAUGHTERING , *RITES & ceremonies , *ESOPHAGUS , *BLOOD vessels - Abstract
Slaughter by Jewish religious rite is the killing of an animal by cutting the trachea and oesophagus and major blood vessels using a very sharp blade. This operation is subject to strict rules laid down by religious authorities that characterize its sacredness. The aim of the study was to evaluate the specific criteria inherent in the Jewish religious rite, by analysing reject rates during the different phases. In this study, 52.4% of the carcasses failed to quality as Kosher , with 22.9% being rejected due to pulmonary lesions and only 3% for miscuts. The study also revealed legal vacuums in the field of labelling rules. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs.
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Palombieri, Andrea, Di Profio, Federica, Lanave, Gianvito, Capozza, Paolo, Marsilio, Fulvio, Martella, Vito, and Di Martino, Barbara
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DOGS , *CANINE parvovirus , *ENTEROVIRUSES , *COVID-19 , *CARNIVOROUS animals , *DIROFILARIA immitis , *FELIDAE - Abstract
• Canine chaphamaparvovirus (CaChPV) is a novel parvovirus recently discovered in dogs; • Herein, stool samples from dogs with or without enteric signs were screened for CaChPV; • CaChPV DNA was found either in diarrhoeic (1.9 %) or asymptomatic (1.6 %) dogs; • The nearly complete genome sequences were determined for two strains; • The Italian CaChPV strains tightly clustered with the American reference viruses. Canine chaphamaparvovirus (CaChPV) is a newly recognised parvovirus discovered by metagenomic analysis during an outbreak of diarrhoea in dogs in Colorado, USA, in 2017 and more recently detected in diarrhoeic dogs in China. Whether the virus plays a role as canine pathogen and whether it is distributed elsewhere, in other geographical areas, is not known. We performed a case-control study to investigate the possible association of CaChPV with enteritis in dogs. CaChPV DNA was detected both in the stools of diarrhoeic dogs (1.9 %, 3/155) and of healthy animals (1.6 %, 2/120). All the CaChPV-infected dogs with diarrhea were mixed infected with other enteric viruses such as canine parvovirus (formerly CPV-2), canine bufavirus (CBuV) and canine coronavirus (CCoV), whilst none of the asymptomatic CaChPV positive animals resulted co-infected. The nearly full-length genome and the partial capsid protein (VP) gene of three canine strains, Te/36OVUD/19/ITA, Te/37OVUD/19/ITA and Te/70OVUD/19/ITA, were reconstructed. Upon phylogenetic analyses based on the NS1 and VP aa sequences, the Italian CaChPV strains tightly clustered with the American reference viruses. Distinctive residues could be mapped to the deduced variable regions of the VP of canine and feline chaphamaparvoviruses, considered as important markers of host range and pathogenicity for parvoviruses. [ABSTRACT FROM AUTHOR]
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- 2020
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