7 results on '"Chia, Suet Lin"'
Search Results
2. Synthesis and in vitro biological evaluations of novel tetrapeptide as therapeutic agent for wound treatment
- Author
-
Fadilah, Nur Izzah Md, Ahmad, Haslina, Abdul Rahman, Mohd Basyaruddin, Chia, Suet Lin, Ng, Shiow-Fern, and Leong, Sze Wei
- Published
- 2020
- Full Text
- View/download PDF
3. Asymmetrical meta-methoxylated diarylpentanoids: Rational design, synthesis and anti-cancer evaluation in-vitro.
- Author
-
Leong, Sze Wei, Chia, Suet Lin, Abas, Faridah, and Yusoff, Khatijah
- Subjects
- *
ANTINEOPLASTIC agents , *ORGANIC synthesis , *STRUCTURE-activity relationship in pharmacology , *CELL lines , *REACTIVE oxygen species - Abstract
Abstract In the present study, a series of forty-five asymmetrical meta -methoxylated diarylpentanoids have been synthesized, characterized and evaluated for their in-vitro anti-cancer potential. Among the forty-five analogs, three compounds (20 , 33 and 42) have been identified as lead compounds due to their excellent inhibition against five human cancer cell lines including SW620, A549, EJ28, HT1080 and MCF-7. Structure-activity relationship study on cytotoxicity of tested compounds suggested that the presence of meta -oxygenated phenyl ring played a critical role in enhancing their cytotoxic effects. Compounds 33 and 42 in particular, exhibited strongest cytotoxicity against tested cell lines with the IC 50 values ranging from 1.1 to 4.3 μM. Subsequent colony formation assay on SW620 cell line showed that both compounds 33 and 42 possessed strong anti-proliferative activity. In addition, flow cytometry based experiments revealed that these compounds could trigger intracellular ROS production thus inducing G2/M-phase cell arrest and apoptosis. All these results suggested that poly meta -oxygenated diarylpentnoid is a promising scaffold which deserved further modification and investigation in the development of natural product-based anti-cancer drug. Graphical abstract Image 1 Highlights • A series of asymmetrical meta -methoxylated diarylpentanoids were synthesized as anti-cancer agents. • Poly- meta -oxygenated analogs 33 and 42 showed potent cytotoxicity against SW620, A549, EJ28, HT1080 and MCF-7 cancer cells. • Analogs 33 and 42 showed anti-proliferative activity. • Analogs 33 and 42 could induce intracellular ROS-mediated G2/M cell arrest and cell apoptosis. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
4. Structural analysis of peptides that interact with Newcastle disease virus
- Author
-
Chia, Suet Lin, Tan, Wen Siang, Shaari, Khozirah, Abdul Rahman, Noorsaadah, Yusoff, Khatijah, and Satyanarayanajois, Seetharama D.
- Subjects
- *
NEWCASTLE disease virus , *PARAMYXOVIRUSES , *VIRUSES , *TISSUE culture - Abstract
Abstract: A peptide with the sequence CTLTTKLYC has previously been identified to inhibit the propagation of Newcastle disease virus (NDV) in embryonated chicken eggs and tissue culture. NDV has been classified into two main groups: the velogenic group, and mesogenic with lentogenic strains as the other group based on its dissociation constants. In this study the peptide, CTLTTKLYC, displayed on the pIII protein of a filamentous M13 phage was synthesized and mutated in order to identify the amino acid residues involved in the interactions with NDV. Mutations of C1 and K6 to A1 and A6 did not affect the binding significantly, but substitution of Y8 with A8 dramatically reduced the interaction. This suggests that Y8 plays an important role in the peptide–virus interaction. The three-dimensional structure of the peptide was determined using circular dichroism (CD), nuclear magnetic resonance (NMR), and molecular modeling. The peptide exhibited two possible conformers. One that consists of consecutive β-turns around T2–L3–T4–T5 and K6–L7–Y8–C9. The other conformer exhibited a β-hairpin bend type of structure with a bend around L3–T4–T5–K6. [Copyright &y& Elsevier]
- Published
- 2006
- Full Text
- View/download PDF
5. Synthesis and in-vitro anti-cancer evaluations of multi-methoxylated asymmetrical diarylpentanoids as intrinsic apoptosis inducer against colorectal cancer.
- Author
-
Leong, Sze Wei, Chia, Suet Lin, Abas, Faridah, and Yusoff, Khatijah
- Subjects
- *
COLORECTAL cancer , *CELL cycle , *CELL analysis , *BAX protein , *CANCER cells , *BCL-2 proteins - Abstract
In the present study, a series of nine stable 3,4,5-methoxylphenyl-containing asymmetrical diarylpentanoids, derivatives of curcuminoids, have been synthesized, characterized and evaluated for their in-vitro anti-cancer potential against a panel of BRAF- and KRAS-mutated colorectal cancer cell lines including T84, LoVo and SW620, HT29, RKO and NCI-H508, respectively. Structure-activity relationship study on cytotoxicity of tested compounds suggested that the presence of meta-hydroxyl and adjacent dimethoxyl groups are crucial for enhanced cytotoxicity of diarylpentanoids. Among the evaluated analogs, 8 has been identified as the lead compound due to its highest chemotherapeutic index of 9.9 and nano molar scale cytotoxicity against SW620 and RKO. Colonies formation and cell cycle analyses on 8 -treated RKO cells showed that 8 exhibits strong anti-proliferative activity by inducing G2/M-phase cell arrest. Subsequent flow cytometry based annexin-V and DCFHDA studies suggested that 8 could induce apoptosis through intracellular ROS-dependent pathway. Further Western blot studies confirmed that 8 has induced intrinsic apoptosis in RKO cells through the up-regulations of Bad and Bax pro-apoptotic proteins and down-regulations of Bcl-2 and Bcl-xL pro-survival proteins. In all, the present results suggest that 8 could be a potent lead which deserves further modification and investigation in the development of small molecule-based anti-colorectal cancer agents. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
6. Newcastle disease virus strain AF2240 as an oncolytic virus: A review.
- Author
-
Kalyanasundram, Jeevanathan, Hamid, Aini, Yusoff, Khatijah, and Chia, Suet Lin
- Subjects
- *
NEWCASTLE disease virus , *CANCER treatment , *APOPTOSIS , *BAX protein , *MACROPHAGES - Abstract
The discovery of tumour selective virus-mediated apoptosis marked the birth of an alternative cancer treatment in the form of oncolytic viruses. Even though, its oncolytic efficiency was demonstrated more than 50 years ago, safety concerns which resulted from mild to lethal side effects hampered the progress of oncolytic virus research. Since the classical oncolytic virus studies rely heavily on its natural oncolytic ability, virus manipulation was limited, thereby, restricted efforts to improve its safety. In order to circumvent such restriction, experiments involving non-human viruses such as the avian Newcastle disease virus (NDV) was conducted using cultured cells, animal models and human subjects. The corresponding reports on its significant tumour cytotoxicity along with impressive safety profile initiated immense research interest in the field of oncolytic NDV. The varying degree of oncolytic efficiency and virulency among NDV strains encouraged researchers from all around the world to experiment with their respective local NDV isolates in order to develop an oncolytic virus with desirable characteristics. Such desirable features include high tumour-killing ability, selectivity and low systemic cytotoxicity. The Malaysian field outbreak isolate, NDV strain AF2240, also currently, receives significant research attention. Apart from its high cytotoxicity against tumour cells, this strain also provided fundamental insight into NDV-mediated apoptosis mechanism which involves Bax protein recruitment as well as death receptor engagement. Studies on its ability to selectively induce apoptosis in tumour cells also resulted in a proposed p38 MAPK/NF-κB/IκBα pathway. The immunogenicity of AF2240 was also investigated through PBMC stimulation and macrophage infection. In addition, the enhanced oncolytic ability of this strain under hypoxic condition signifies its dynamic tumour tropism. This review is aimed to introduce and discuss the aforementioned details of the oncolytic AF2240 strain along with its current challenges which outlines the future research direction of this virus. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
7. Discovery of a novel dual functional phenylpyrazole-styryl hybrid that induces apoptotic and autophagic cell death in bladder cancer cells.
- Author
-
Leong, Sze Wei, Wang, JingJing, Okuda, Kazuhide Shaun, Su, Qi, Zhang, Yanmin, Abas, Faridah, Chia, Suet Lin, and Yusoff, Khatijah
- Subjects
- *
CELL death , *CANCER cells , *BLADDER cancer , *ANTINEOPLASTIC agents , *EPIDERMAL growth factor receptors , *CANCER cell culture - Abstract
Unpleasant side effects and resistance development remained the Achilles heel of chemotherapy. Since low tumor-selectivity and monotonous effect of chemotherapy are closely related to such bottleneck, targeting tumor-selective multi-functional anticancer agents may be an ideal strategy in the search of new safer drugs. Herein, we report the discovery of compound 21 , a nitro-substituted 1,5-diphenyl-3-styryl-1H-pyrazole that possesses dual functional characteristics. The 2D- and 3D-culture-based studies revealed that 21 not only could induce ROS-independent apoptotic and EGFR/AKT/mTOR-mediated autophagic cell deaths in EJ28 cells simultaneously but also has the ability in inducing cell death at both proliferating and quiescent zones of EJ28 spheroids. The molecular modelling analysis showed that 21 possesses EGFR targeting capability as it forms stable interactions in the EGFR active site. Together with its good safety profile in the zebrafish-based model, the present study showed that 21 is promising and may lead to the discovery of tumor-selective multi-functional anti-cancer agents. [Display omitted] • A series of phenylpyrazole-styryl hybrids were synthesized as anti-cancer agents. • 21 shows potent activity against EJ28 with excellent safety profile in zebrafish. • 21 induces apoptotic and autophagic cell death in EJ28 simultaneously. • 21 downregulates EGFR in-vitro and interacts stably with EGFR in-silico. • 21 induces cell death in proliferating and quiescent zones of EJ28 spheroids. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.