Ronca, Roberto, Giacomini, Arianna, Di Salle, Emanuela, Coltrini, Daniela, Pagano, Katiuscia, Ragona, Laura, Matarazzo, Sara, Rezzola, Sara, Maiolo, Daniele, Torrella, Rubben, Moroni, Elisabetta, Mazzieri, Roberta, Escobar, Giulia, Mor, Marco, Colombo, Giorgio, and Presta, Marco
Summary The fibroblast growth factor (FGF)/FGF receptor (FGFR) system plays a crucial role in cancer by affecting tumor growth, angiogenesis, drug resistance, and escape from anti-angiogenic anti-vascular endothelial growth factor therapy. The soluble pattern recognition receptor long-pentraxin 3 (PTX3) acts as a multi-FGF antagonist. Here we demonstrate that human PTX3 overexpression in transgenic mice driven by the Tie2 promoter inhibits tumor growth, angiogenesis, and metastasis in heterotopic, orthotopic, and autochthonous FGF-dependent tumor models. Using pharmacophore modeling of the interaction of a minimal PTX3-derived FGF-binding pentapeptide with FGF2, we identified a small-molecule chemical (NSC12) that acts as an extracellular FGF trap with significant implications in cancer therapy. [ABSTRACT FROM AUTHOR]