Search

Research is needed to look for plants or lines that can thrive on soils affected by water deficit or other constraints. The present work investigated the potentiality of Saccharum spontaneum L. spp. aegyptiacum (Willd.) Hack., a lignocellulosic, perennial, rhizomatous, no-food crop in semi-arid Mediterranean area. A 3-year field trial was carried out with the aim to evaluate its physiology, biomass yield, water use efficiency, and biomass quality under different soil water availability. During 2011/2012, 2012/2013, and 2013/2014 growing seasons (hereinafter referred as 2011, 2012, and 2013, respectively), three levels of maximum evapotranspiration restitution (ETm) were compared: I 100 (100% ETm restitution), I 50 (50% ETm restitution), and I 0 (rainfed condition). Net photosynthesis, stomatal conductance, and transpiration rate were strictly related to the available soil water content (ASWC), with maximum gas exchange at field capacity. However, gas exchange between plants and atmosphere was also measured when ASWC was close to wilting point. Biomass dry matter (DM) yield and main morpho-biometric traits (e.g., stem height and basal stem diameter) were significantly affected by ASWC. A non-linear model showed that maximum DM yield, 37.86 Mg ha −1 , can be achieved when 1150 mm of water were used by the crop. Water use efficiency (WUE) was always highest in rainfed condition ( I 0 ), however, only in 2013 I 0 was significantly higher than I 50 and I 100 (5.89 g L −1 vs 4.26 and 3.33 g L −1 ). Within the variables employed, a linear model showed that WUE was negatively related to crop water use (CWU). The fiber content was not affected by the irrigation treatments. In the average, hemicellulose, cellulose, and lignin accounted for 23.9 (% w/w), 35.0 (% w/w), and 19.9 (% w/w), respectively. On the other hand, moisture and ash content, bulk density, low heating value, and ash melting point resulted significantly affected by the treatment. Present results suggested that S. spontaneum spp. aegyptiacum possesses a range of agronomically desirable traits of biomass crop: C4 plant, high biomass yield, active assimilation rates during drought–stress periods, able to use water efficiently, and satisfactory biomass quality. [ABSTRACT FROM AUTHOR]

Abstract: The research of alternative crops for biomass production for energy indicates giant reed (Arundo donax L.), widespread spontaneous plant in Mediterranean regions, among the species at high aptitude for accumulation of biomass. Within the activity of an E.U. programme (CEE FAIR CT 97-2028 “Giant reed (A. donax L.) Network. Improvement, productivity and biomass quality”, germplasm of giant reed were collected to evaluate potential production and the phenotypic and genotypic variability, the heritability in order to selecting the best genotypes. In 1997 and 1998, trials were carried out in Primosole site (Piana of Catania, sea level, 37°25′N latitude; 15°30′E longitude), utilizing rhizomes of 39 clones collected in Sicily and Calabria. The rhizomes were transplanted in springtime. Phenological (date of flowering), biometrical (stem density, stem height, number of nodes per stem, diameter and thickness of stems, weight of fresh and dry biomass of leaves, stems and inflorescence) and productive (yield) data were measured. Harvest were carried out in February 1988 and 1989. Yield of 39 clones studied was, in the average, 10.6tha−1 of dry matter in the first year and 22.1tha−1 in the second one. The clone no. 4 (Piazza Armerina) and the clone no. 20 (Capo d’Orlando) maintained their high productive aptitude in both years; they yielded respectively, 13.1 and 14.1tha−1 in the first year and 34.2 and 26.9tha−1 in the second one. The yield results positively correlated to stem density, stem weight and plant height. Four characters: biomass yield, stem weight, stem density and stem height showed a significant variance among clones without significant interaction with year. Among the eleven characters measured only yield, stem weight, stem density and stem height had moderate heritability (h 2), comprised between 23 and 48% showing promise for genetic improvement. [Copyright &y& Elsevier]

Sustainable biomass production mostly relies on cultivation practices employing low external input supply. Wild germplasm might be suited for low-input techniques while providing enough output. The present study investigated four native Mediterranean perennial grasses ( Oryzopsis miliacea , Cymbopogon hirtus , Sorghum halepense , and Saccharum spontaneum L. ssp. aegyptiacum ), with an autumn and winter harvest regime in a 4-year field trial in Sicily (south of Italy). Species, cultivation year and harvest time had significant effects on whole season crop water use efficiency, energy efficiency and biomass quality. Species and cultivation year also significantly affected aboveground biomass yield and net energy yield. The total accumulated harvested yield over the 4-year experiment was the highest in Saccharum in both autumn and winter harvests (79.5 and 79.2 Mg DM ha −1 ), and the lowest in Cymbopogon (13.7 and 14.9 Mg DM ha −1 ). In both harvests, less than 20% of this total biomass was collected for all species at the first year (9.5–11.3% in Sorghum and 15.7–18.1% in Oryzopsis ). Peak biomass was reached at the third ( Saccharum and Sorghum ) or at the fourth year ( Orzyzopsis and Cymbopogon ) in autumn, and at the third year in winter harvest (all species). Water use efficiency was higher in the autumn than in the winter harvest (2.06 and 1.75 g L −1 , respectively), whilst the opposite was observed for energy efficiency (35.7 and 38.6 GJ ha −1 , respectively). Biomass structural compounds (hemicellulose, cellulose and acid detergent lignin) were higher in winter than autumn, while protein, lipid and ash contents were higher in autumn than winter. [ABSTRACT FROM AUTHOR]

Highlights • Propagation by single-node stem cutting of giant reed (Arundo donax) was attempted. • Nutrients availability on the node plays a key role in rooting development. • In open field, the best period for node excision is July followed by May. • Nodes whose diameter exceed 2 cm showed higher rooting and plantlets development. • IBA resulted effective in promoting rooting in the worst excision dates. Abstract Giant reed (Arundo donax L.) is a warm-season, fast growing lignocellulosic crop suitable to Mediterranean and semi-arid environments. However, its sterility limits a cost-effective propagation along with environmental impacts associated with rhizome processing. In the present study, the effects of intrinsic (node size and node chemical composition) and extrinsic factors (node pretreatment with growth regulators) on root and shoot development were investigated under different excision time of node-cuttings in a controlled and open field conditions. Results indicated that nutrients availability of the propagation material plays a key role in rooting development. To enhance the success of giant reed propagation by single node stem-cuttings, the best period for node excision is July in open field trial, followed by the late spring (May). Nodes whose diameter exceed 2 cm resulted in higher rooting and plantlets development even in the less favorable excision dates. However, when big nodes are adopted in controlled environments even winter periods are eligible, being particularly useful for spring open field transplants of rooted plantlets. Among the growth regulators used in the present study, indole-3-butyric acid - IBA (in controlled environment) resulted effective in promoting rooting particularly in the worse excision date (April); in open field, IBA significantly improved rooting in the excision dates of February and May in the big nodes, and only in February in the small ones. NAA (1-naphthaleneacetic acid) was almost ineffective in enhancing rooting as compared to the control. The present study might open a new perspective to overcome one of the most significant drawbacks to establish giant reed, namely its agamic propagation. [ABSTRACT FROM AUTHOR]

Within a framework of renewed interest in crambe ( Crambe abyssinica Hochst ex R.E. Fries) sourcing raw materials for the bio-based industry, the adaptability and productive performances of this oil crop have been evaluated under contrasting Mediterranean environments (i.e., a fertile site in the northern part of Po valley vs. a semi-arid site of southern Sicily) during two consecutive growing seasons, aiming at its possible stable introduction in this area. The trial set in northern Italy compared three commercial varieties of crambe (Galactica, Nebula and Mario) in spring sowing, while in southern Italy only the var. Mario was tested with autumn sowing. Regardless of location and variety, thermal time for maturity was quite stable (1200–1400 °C), and the crop provided satisfactory seed yields (grand mean 2.29 Mg hulled seeds ha −1 ), with average oil content of ∼400 g kg −1 (on dehulled seeds) and ∼52% of erucic acid. Significantly higher seed and oil yields were reached in northern than in southern Italy. Furthermore, crambe thermal use efficiency (THUE) was also higher in the north than in the south, possibly due to better environmental adaptability of the crop. The limited intraspecific variability within crambe was confirmed, with better productive performances showed by the domestic selection Mario. Promising traits were revealed in Nebula, showing greater seed weight, root length density and area, and thinner roots, although the root growth of crambe was generally modest compared with modern high erucic acid rapeseed hybrids. Available crambe varieties could be efficiently included in crop rotations across a wide range of environments within the Mediterranean basin. The short growth cycle represents an outstanding added value for this species, allowing the avoidance of prolonged drought and heat stress typical of late spring/early summer months under the Mediterranean climate. However, increased yields are needed to meet the market requests; nonetheless, the little genetic variability suggests that there is large scope for future breeding improvements, maybe exploiting advanced techniques to improve the existing genetic resources. [ABSTRACT FROM AUTHOR]

The aim of the present work was to evaluate the effect of soil water availability and nitrogen fertilization on yield, water use efficiency and agronomic nitrogen use efficiency of giant reed ( Arundo donax L.) over four-year field experiment. After the year of establishment, three levels for each factor were studied in the following three years: I 0 (irrigation only during the year of establishment), I 1 (50% ETm restitution) and I 2 (100% ETm restitution); N 0 (0 kg N ha −1 ), N 1 (60 kg N ha −1 ) and N 2 (120 kg N ha −1 ). Irrigation and nitrogen effects resulted significant for stem height and leaf area index (LAI) before senescence, while no differences were observed for stem density and LAI at harvest. Aboveground biomass dry matter (DM) yield increased following the year of establishment in all irrigation and N fertilization treatments. It was always the highest in I 2 N 2 (18.3, 28.8 and 28.9 t DM ha −1 at second, third and fourth year growing season, respectively). The lowest values were observed in I 0 N 0 (11.0, 13.4 and 12.9 t DM ha −1 , respectively). Water use efficiency (WUE) was significantly higher in the most stressed irrigation treatment (I 0 ), decreasing in the intermediate (I 1 ) and further in the highest irrigation treatment (I 2 ). N fertilization lead to greater values of WUE in all irrigation treatment. The effect of N fertilization on agronomic nitrogen use efficiency (NUE) was significant only at the first and second growing season. Giant reed was able to uptake water at 160–180 cm soil depth when irrigation was applied, while up to 140–160 cm under water stress condition. Giant reed appeared to be particularly suited to semi-arid Mediterranean environments, showing high yields even in absence of agro-input supply. [ABSTRACT FROM AUTHOR]

Tissue culture methods offer the potential for large-scale propagation of giant reed ( Arundo donax L.), a promising crop for energy biomass. In previous trials, giant reed resulted particularly suitable to in vitro culture. In this paper, with the final goal of enhancing the efficiency of in vitro production process and reducing costs, the influence of four different culture media (agar or gellan-gum solidified medium, liquid medium into a temporary immersion system-RITA ® or in a stationary state) on in vitro shoot proliferation of giant reed was evaluated. Giant reed exhibited a particular sensitivity to gelling agents during the phase of secondary shoot formation. Gellan gum, as compared to agar, improved the efficiency of in vitro culture giving more shoots with higher mean fresh and dry weight. Moreover, the cultivation of this species into a liquid medium under temporary immersion conditions or in a stationary state, was comparatively as effective as and cheaper than that into a gellan gum medium. Increasing 6-benzylaminopurine (BA) up to 4 mg l −1 also resulted in a further enhancement of secondary shoot proliferation. The good adaptability of this species to liquid medium and the high multiplication rates observed indicate the possibility to obtain from a single node at least 1200 plantlets every six multiplication cycles (about 6 months), a number 100 fold higher than that obtained yearly per plant by the conventional methods of vegetative multiplication. In open field, micropropagated plantlets guaranteed a higher number of survived plants, secondary stems and above ground biomass as compared to rhizome ones. [ABSTRACT FROM AUTHOR]

Highlights: [•] Monoecious and dioecious hemp were studied in Mediterranean semi-arid environment. [•] Later dioecious showed the highest aboveground and stem DM yield (tha−1) [•] WUE ranged between 2.73gl−1 in good water and 3.45gl−1 in water stress conditions. [•] Optimum of photosynthetic rate was achieved at 24°C. At 43°C hemp growth ceased. [•] As soil water availability increased, RUE increased and light extinction coefficient (k) decreased. [ABSTRACT FROM AUTHOR]

Abstract: Fibre hemp can be grown under a wide range of agro-ecological conditions, but it requires special attention for several physiological features and crop management. A management strategy in order to reduce inputs and thus achieve acceptable yield could be achieved optimizing sowing time. With this respect, the effects of sowing date on hemp biology and yield was studied, using two monoecious and two dioecious genotypes. Field experiments were carried out in two subsequent years (2003–2004) in South of Italy, using drip irrigation system. Sowing time, in the two year period, ranged between March 10th and July 22nd. Optimal sowing time was observed between the end of April and the first three weeks of May; in that range, the dioecious Fibranova yielded the most in terms of aboveground biomass and stem dry yield, followed by Tiborszallasi, while the two monoecious showed the lowest yield. On the contrary, before and after that period, the shorter day length caused an early floral induction that strongly reduced stem and fibre elongation, and thus aboveground dry biomass and consequently stem yields. Based on this study a simulation model focusing on flowering prediction in Mediterranean environment was developed. [Copyright &y& Elsevier]

Abstract: Miscanthus × giganteus is one of the most promising biomass crops for non-food utilisation. Taking into account its area of origin (Far East), its temperature and rainfall requirements are not well satisfied in Mediterranean climate. For this purpose, a research was carried out with the aim of studying the adaptation of the species to the Mediterranean environment, and at analysing its ecophysiological and productive response to different soil water and nitrogen conditions. A split plot experimental design with three levels of irrigation (I1, I2 and I3 at 25%, 50% and 100% of maximum evapotranspiration (ETm), respectively) and three levels of nitrogen fertilisation (0kgha−1: N0, 60kgha−1: N1 and 120kgha−1: N2 of nitrogen) were studied. The crop showed a high yield potential under well-watered conditions (up to 27tha−1 of dry matter). M. × giganteus, in Mediterranean environment showed a high yield potential even in very limited water availability conditions (more than 14tha−1 with a 25% ETm restoration). A responsiveness to nitrogen supply, with great yield increases when water was not limiting, was exhibited. Water use efficiency (WUE) achieved the highest values in limited soil water availability (between 4.51 and 4.83gl−1), whilst in non-limiting water conditions it decreased down to 2.56 and 3.49gl−1 (in the second and third year of experiment, respectively). Nitrogen use efficiency (NUE) decreased with the increase of water distributed (from 190.5gg−1 of I0 to 173.2gg−1 of I2); in relation to N fertilisation it did not change between the N fertilised treatments (N1 and N2), being much higher in the unfertilised control (177.1gg−1). Radiation use efficiency (NUE) progressively declined with the reduction of the N fertiliser level (1.05, 0.96 and 0.86gd.m.MJ−1, in 1994, and 0.92, 0.91 and 0.69gd.m.MJ−1, in 1995, for N2, N1 and N0, respectively). [Copyright &y& Elsevier]