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Abstract: The experiment was conducted to investigate the effects of two homofermentative lactic acid bacteria (LAB) (two strains of Lactobacillus plantarum and multi-strain of homofermentative LAB+enzyme mixture) and a heterofermentative LAB (two strains of Lactobacillus buchneri) on conservation characteristics of baled triticale–Hungarian vetch silage and lamb performance. Triticale–Hungarian vetch mixture was mowed at milk stage of maturity of triticale. The herbage was wilted to 473g/kg dry matter (DM), treated with LAB additives at 1.0×106 cfu/g and baled. Bales were wrapped with six layers of plastic white stretch-film and stored outdoor for 3 months. The control and inoculated silages were preserved well at pH values of between 4.5 and 4.6 with low concentration of butyric acid, moderately low concentration of ammonia-N and low count of yeast and mould. The homofermentative LAB either used alone or in combination with enzyme mixture had little effect on preservation characteristics of baled silages. However, L. buchneri had a more profound effect on the final fermentation products of baled silage and improved the standard of preservation with the apparently difficult-to-preserve herbage used in this experiment. Inoculation with L. buchneri resulted in silage with higher (P<0.05) concentration of acetic and propionic acid and lower (P<0.05) concentration of lactic acid and water soluble carbohydrates (WSC). Aerobic stability was the longest (P<0.05) in silage treated with L. buchneri, but it was the shortest (P<0.05) in silages treated with L. plantarum. Lambs fed with the silages treated with L. buchneri had higher (P<0.01) silage and total intake. There was no (P>0.05) treatment effect on any variables measured on rumen fluid. It can be concluded that L. buchneri can offer to make bale conditions more inhibitory to activities of undesirable microorganism and may positively affect the feeding value of silages. [Copyright &y& Elsevier]

Cobalt chloride (CoCl2) is one of the most reactive catalysts in the hydrolysis of sodium borohydride (NaBH4). The present study reports hydrogen release results for NaBH4 一CoCl2 systems, both solids having been mixed together via a ‘solvent’ route. Actually, the ‘solvent’ route using tetrahydrofuran (THF), compared to the mechanical mixing (‘mortar’ route), improves the CoCl2 reactivity in terms of catalyst activation time, hydrogen generation rate (HGR) and total conversion. SEM and XRD observations suggest that the ‘THF’ route favors the ex-situ reduction of Co2+ to an active cobalt phase. Other metal (cobalt or ruthenium) salts are also reported. [Copyright &y& Elsevier]

Intestinal transplantation is the most effective treatment for patients with short bowel syndrome and small bowel insufficiencies. We evaluated epithelial chimerism after infusion of autologous bone marrow mesenchymal stromal cells (BMSCs) in patients undergoing cadaveric donor isolated intestinal transplantation (I-ITx). BMSCs were isolated from patients' bone marrow via iliac puncture and expanded in vitro prior to infusion. Two out of the 3 patients were infused with autologous BMSCs, and small intestine tissue biopsies collected post-operatively were analyzed for epithelial chimerism using XY fluorescent in situ hybridization and short tandem repeat polymerase chain reaction. We observed epithelial chimeric effect in conditions both with and without BMSC infusion. Although our results suggest a higher epithelial chimerism effect with autologous BMSC infusion in I-ITx, the measurements in multiple biopsies at different time points that demonstrate the reproducibility of this finding and its stability or changes in the level over time would be beneficial. These approaches may have potential implications for improved graft survival, lower immunosuppressant doses, superior engraftment of the transplanted tissue, and higher success rates in I-ITx. [ABSTRACT FROM AUTHOR]