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A simple but effective near-infrared ratiometric fluorescent probe for hydrazine has been developed by a hybrid fluorophore of coumarin and benzopyrylium. The recognition mechanism involving spirocyclic ring-opening and −closing toward hydrazine is first reported. The probe showed high selectivity and sensitivity toward hydrazine with lower detection limit (1.5 ppb) under single wavelength excitation. Upon addition of trace amounts of hydrazine, the probe showed a remarkable ratiometric change in the absorption (Δλ = 167 nm) and emission (Δλ = 201 nm) spectra and a color change from green to yellow. Furthermore, the probe-loaded TLC plates were made to detect vapor of hydrazine by naked eyes. Moreover, this ratiometric probe was successfully used for the imaging of hydrazine in fetal bovine serum and living cells. [ABSTRACT FROM AUTHOR]

We have developed the first two-photon colorimetric and ratiometric fluorescent probe, BICO , for the detection of bisulfite (HSO 3 − ) in aqueous solution. The probe contains coumarin and benzimidazole moieties and can detect HSO 3 − based on the Michael addition reaction with a limit of detection 5.3 × 10 −8 M in phosphate-buffered saline solution. The probe was used to detect bisulfite in tap water, sugar and dry white wine. Moreover, test strips were made and used easily. We successfully applied the probe to image living cells, using one-photon fluorescence imaging. BICO overcomes the limitations in sensitivity of previously reported probes and the solvation effect of bisulfite, which demonstrates its excellent value in practical application. [ABSTRACT FROM AUTHOR]