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Anthropogenic inputs of petroleum hydrocarbons into the marine environment can have long lasting impacts on benthic communities. Sponges form an abundant and diverse component of benthic habitats, contributing a variety of important functional roles; however, their responses to petroleum hydrocarbons are largely unknown. This study combined a traditional ecotoxicological experimental design and endpoint with global gene expression profiling and microbial indicator species analysis to examine the effects of a water accommodated fraction (WAF) of condensate oil on a common Indo-Pacific sponge, Phyllospongia foliascens. A no significant effect concentration (N(S)EC) of 2.1 % WAF was obtained for larval settlement, while gene-specific (N(S)EC) thresholds ranged from 3.4 % to 8.8 % WAF. Significant shifts in global gene expression were identified at WAF treatments ≥20 %, with larvae exposed to 100 % WAF most responsive. Results from this study provide an example on the incorporation of non-conventional molecular and microbiological responses into ecotoxicological studies on petroleum hydrocarbons. • Traditional and non-traditional responses to oil at similar threshold values • Larvae exposed to highest oil treatments show greatest gene expression changes • Genes potentially associated with hydrocarbon detoxification in larvae identified • Indicator microbes associated with sponge larvae identified in high oil treatments [ABSTRACT FROM AUTHOR]

High-intensity, impulsive sounds are used to locate oil and gas reserves during seismic exploration of the seafloor. The impacts of this noise pollution on the health and mortality of marine invertebrates are not well known, including the silverlip pearl oyster (Pinctada maxima), which comprises one of the world's last remaining significant wildstock pearl oyster fisheries, in northwestern Australia. We exposed ≈11,000 P. maxima to a four-day experimental seismic survey, plus one vessel-control day. After exposure, survival rates were monitored throughout a full two-year production cycle, and the number and quality of pearls produced at harvest were assessed. Oysters from two groups, on one sampling day, exhibited reduced survival and pearl productivity compared to controls, but 14 other groups receiving similar or higher exposure levels did not. We therefore found no conclusive evidence of an impact of the seismic source survey on oyster mortality or pearl production. [Display omitted] • Largescale experimental seismic survey exposes adult pearl oysters to 209 dB re 1 µPa2·s. • Seismic source survey alone very unlikely to cause mortality of Pinctada maxima • No correlation: little effect of sound levels on P. maxima cultured pearl productivity • Reduced survival/pearl retention on one sampling day unlikely driven by seismic exposure [ABSTRACT FROM AUTHOR]

Application of oil toxicity modelling for assessing the risk of spills to coral reefs remains uncertain due to a lack of data for key tropical species and environmental conditions. In this study, larvae of the coral Acropora millepora were exposed to six aromatic hydrocarbons individually to generate critical target lipid body burdens (CTLBBs). Larval metamorphosis was inhibited by all six aromatic hydrocarbons, while larval survival was only affected at concentrations >2000 μg L−1. The derived metamorphosis CTLBB of 9.7 μmol g−1 octanol indicates larvae are more sensitive than adult corals, and places A. millepora larvae among the most sensitive organisms in the target lipid model (TLM) databases. Larvae were also more sensitive to anthracene and pyrene when co-exposed to ecologically relevant levels of ultraviolet radiation. The results suggest that the application of the phototoxic TLM would be protective of A. millepora larvae, provided adequate chemical and light data are available. [Display omitted] • Effects of six aromatic hydrocarbons on coral larvae were assessed ±UV radiation. • The first species-specific sensitivity constant for coral larvae was derived. • Results allow the toxicity of any oil towards coral larvae to be predicted. • Coral larvae are among the most sensitive taxa investigated to date. • Phototoxic target lipid model predictions are likely protective of larvae. [ABSTRACT FROM AUTHOR]

Background: Regulatory decisions approving new coronary drug-eluting stent (DES) require mechanistic observations of angiographic late lumen loss (LLL). Patient safety and device approval times could be enhanced if angiographic follow-up data were found to be generalizable across jurisdictions and geographies. The objectives were to assess the comparability of in-segment LLL in Eastern and Western DES populations using the world's largest compilation of follow-up quantitative coronary angiography data.Methods: Data from 4 manufacturers involving 29 DES clinical trials in Eastern and Western hemispheres were compiled. "East" and "West" cohorts were defined by trial location. Independent core laboratories quantified in-segment LLL for all studies. East and West were compared before and after adjustment for clinical and anatomic covariates known to correlate with LLL via conditioning on propensity score quintiles. An international panel of experts and regulators prospectively established a clinically meaningful difference between East and West mean in-segment LLL of ±0.40 mm.Results: The data set comprised 2,047 East and 4,456 West patients. Unadjusted mean ± SD for West and East in-segment LLL (mm) was 0.25 ± 0.46 and 0.12 ± 0.42, respectively (difference 0.13 mm; 95% CI 0.11-0.16). Propensity score-adjusted in-segment LLL East and West least squares means were 0.11 and 0.26 mm, respectively (difference 0.15 mm; 95% CI 0.13-0.18).Conclusions: In the world's largest compilation of DES protocol 8- to 13-month angiographic follow-up data, clinically meaningful comparability of in-segment LLL by independent core laboratory quantitative coronary angiography in East and West cohorts was demonstrated in both unadjusted and adjusted comparisons. These findings suggest that DES LLL, once characterized, could be generalized across regulatory jurisdictions over the course of global registration efforts. [ABSTRACT FROM AUTHOR]

Purpose: To determine whether use of a ketogenic formula during the first month of the modified Atkins diet (MAD) in adults with drug-resistant epilepsy (DRE) improves seizure reduction and compliance compared to MAD alone.Methods: Eighty adults (age ≥18 years) with DRE and ≥4 reliably quantifiable seizures/month were enrolled. All participants were trained to follow a 20 g/day net carbohydrate limit MAD. Patients were randomized to receive one 8-ounce (237 mL) tetrapak of KetoCal®, a 4:1 ketogenic ratio formula, daily in combination with MAD during the first month (treatment arm) or second month (control/cross-over arm). Patients recorded urine ketones, weight, and seizure frequency and followed up at 1 and 2 months.Results: By 1 month, 84% of patients achieved ketosis (median of 4-4.5 days). At 1 month, the treatment arm had a significantly higher ketogenic ratio and more patients with a ≥1:1 ketogenic ratio compared to the control arm. There was no difference in median seizure frequency, proportion of responders (≥50% seizure reduction), or median seizure reduction from baseline between groups. However, patients treated with KetoCal® during the first month were significantly more likely to continue MAD for 6 months or more.Conclusion: Although supplementing MAD with a ketogenic formula in the first month did not increase the likelihood of reducing seizures compared to MAD alone, significantly more adults remained on MAD long-term with this approach. This suggests a potential strategy for encouraging compliance with MAD in adults with DRE. [ABSTRACT FROM AUTHOR]

Changes in turbidity, sedimentation and light over a two year large scale capital dredging program at Onslow, northwestern Australia, were quantified to assess their effects on filter feeder communities, in particular sponges. Community functional morphological composition was quantified using towed video surveys, while dive surveys allowed for assessments of species composition and chlorophyll content. Onslow is relatively diverse recording 150 sponge species. The area was naturally turbid (1.1 mean P 80 NTU), with inshore sites recording 6.5 × higher turbidity than offshore localities, likely influenced by the Ashburton River discharge. Turbidity and sedimentation increased by up to 146% and 240% through dredging respectively, with corresponding decreases in light levels. The effects of dredging was variable, and despite existing caveats (i.e. bleaching event and passing of a cyclone), the persistence of sponges and the absence of a pronounced response post-dredging suggest environmental filtering or passive adaptation acquired pre-dredging may have benefited these communities. [ABSTRACT FROM AUTHOR]

Some coral species of the genus Porites can produce thick mucous sheets that partially or completely envelope the colony's surface. This phenomenon has been reported many times, but the cause and ecological significance remains unclear. In this study, sheet production was examined in response to elevated suspended sediment concentrations associated with a large-scale, extended dredging project on a coral reef. Approximately 400 corals at 16 locations situated from 0.2-33 km from the excavation area were examined at fortnightly intervals over the 1.5 year dredging campaign. Mucous sheets were observed on 447 occasions (from 10,600 observations), with average mucous prevalence ranging from 0-10%. Overall 74 ± 5% of the colonies <1.5 km from the dredging produced one or more sheets. High levels of mucous coverage (≥95% of the colony surface) was observed on 68 occasions, and 82% of these occurred at sites close to the dredging. Approximately 50% of colonies produced ≥3 sheets over the monitoring period, and 90% of these were located close to the dredging. In contrast, at distantly located reference sites (>20 km away), mean mucous sheet prevalence was very low (0.2% ± 0.1), no colonies produced more than 1 sheet, and only 1 colony was observed with high mucous coverage. In a laboratory-based experiment, explants of Porites spp. exposed to fine silt also produced mucous sheets (105 sheets recorded in 1100 observations), with nearly 30% of the fragments exposed to repeated sediment deposition events of 10 and 20 mg cm-2 d-1 producing 2 new sheets over the 28 day exposure period. These multiple lines of evidence suggest a close association between mucous sheet formation and sediment load, and that sheet formation and sloughing are an additional mechanism used by massive Porites spp. to clear their surfaces when sediment loads become too high. These results suggest that mucous sheet formation is an effective bioindicator of sediment exposure. [ABSTRACT FROM AUTHOR]

As part of an investigation of the effects of water quality from dredging/natural resuspension on reefs, the effects of suspended sediment concentrations (SSCs) (0, 30, 100 mg L − 1 ) and light (~ 0, 1.1, 8.6 mol photons m − 2 d − 1 ) were examined alone and in combination, on the corals Acropora millepora , Montipora capricornis and Porites spp. over an extended (28 d) period. No effects were observed at any sediment concentrations when applied alone. All corals in the lowest light treatments lost chlorophyll a and discoloured (bleached) after a week. Coral mortality only occurred in the two lowest light treatments and was higher when simultaneously exposed to elevated SSCs. Compared to water quality data collected during large dredging programs and natural resuspension events (and in the absence of sediment deposition as a cause-effect pathway) these data suggest the light reduction associated with turbidity poses a proportionally greater risk than effects of elevated SSCs alone. [ABSTRACT FROM AUTHOR]

Background: In the stroke prevention trials of left atrial appendage closure with the Watchman device (Boston Scientific), a postimplantation antithrombotic regimen of 6 weeks of warfarin was used.Objective: Given the clinical complexity of warfarin use, the purpose of this study was to study the relative feasibility and safety of using non-warfarin oral anticoagulants (NOACs) instead of warfarin during the peri- and initial postimplantation periods after Watchman implantation.Methods: This was a retrospective multicenter study of consecutive patients undergoing Watchman implantation and receiving peri- and postprocedural NOACs or warfarin. Transesophageal echocardiography or chest computed tomography was performed between 6 weeks and 4 months postimplant to assess for device-related thrombosis. Bleeding and thromboembolic events also were evaluated at the time of follow-up.Results: In 5 centers, 214 patients received NOACs (46% apixaban, 46% rivaroxaban, 7% dabigatran, and 1% edoxaban) in either an uninterrupted (82%) or a single-held-dose (16%) fashion. Compared to a control group receiving uninterrupted warfarin (n = 212), the rates of periprocedural complications, including bleeding events, were similar (2.8% vs 2.4%, P = 1). At follow-up, the rates of device-related thrombosis (0.9% vs 0.5%, P = 1), composite of thromboembolism or device-related thrombosis (1.4% vs 0.9%, P = 1), and postprocedure bleeding events (0.5% vs 0.9%, P = .6) also were comparable between the NOAC and warfarin groups.Conclusion: NOACs proved to be a feasible peri- and postprocedural alternative regimen to warfarin for preventing device-related thrombosis and thromboembolic complications expected early after appendage closure with the Watchman device, without increasing the risk of bleeding. [ABSTRACT FROM AUTHOR]

Background: The contemporary evaluation of novel drug-eluting stents (DES) includes mechanistic observations that characterize postdeployment stent behavior. Quantification of late lumen loss due to neointimal hyperplasia 8-13 months after stent implantation, via quantitative coronary angiography (QCA), constitutes such an observation and is required by most regulatory authorities. Late lumen loss, as determined by QCA, has been validated as a surrogate for clinical endpoints such as target vessel revascularization. The mechanistic response to DES has not been directly compared across predominantly Asian or Western populations, whereas understanding their comparability across geographic populations could enhance global DES evaluation.Objective: The East-West late lumen loss study is designed to demonstrate whether the residual differences in late lumen loss, as assessed by QCA, is different between Eastern and Western DES recipients from studies with protocol angiography at 8-13 months of follow-up.Methods: Data from independent core laboratories that have characterized angiographic late lumen loss in DES clinical trials with protocol follow-up angiography will be compiled and dichotomized into Eastern and Western populations. A prospectively developed propensity score model incorporating clinical and anatomic variables affecting late lumen loss will be used to adjust comparisons of QCA measurements.Conclusion: Documentation of whether there are clinically meaningful differences in mechanistic response to DES implantation across genetically unique geographies could facilitate both the quality and efficiency of global device evaluation requiring invasive follow-up for novel stent designs. [ABSTRACT FROM AUTHOR]

There is a need to developwater quality thresholds for dredging near coral reefs that can relate physical pressures to biological responses and define exposure conditions abovewhich effects could occur.Water quality characteristics during dredging have, however, not been well described. Using information from several major dredging projects, we describe sediment particle sizes in the water column/seabed, suspended sediment concentrations at different temporal scales during natural and dredging-related turbidity events, and changes in light quantity/ quality underneath plumes. These conditions differ considerably from those used in past laboratory studies of the effects of sediments on corals. The reviewalso discusses other problems associatedwith using information from past studies for developing thresholds such as the existence of multiple different and inter-connected cause-effect pathways (which can confuse/confound interpretations), the use of sediment proxies, and the reliance on information from sediment traps to justify exposure regimes in sedimentation experiments. [ABSTRACT FROM AUTHOR]

Highlights: [•] Landscape management may influence resource availability for threatened species. [•] Food resources for an endangered bird were influenced by time since fire. [•] A high proportion of habitat was outside the estimated optimum time since fire. [•] Manipulating burning patterns may enhance resources for threatened species. [•] Successful species conservation will require complex management trade-offs. [ABSTRACT FROM AUTHOR]

Significant metacognitive impairments are observed in chronic psychosis samples but metacognition is less understood in first episode psychosis (FEP). The current study explored correlations between metacognition, symptoms and premorbid functioning in an FEP sample. In a cross-sectional cohort study, individuals in the first 12 months of treatment metacognition were assessed with the Metacognition Assessment Scale-Revised version (MAS-R). Psychotic symptomatology, premorbid adjustment, and clinician rated service engagement were also measured. Lower scores for metacognitive understanding of other's minds were significantly correlated with greater negative symptoms, poorer early adolescent social adjustment and poorer clinician rated help-seeking. Our findings suggest that FEP individuals with difficulties in understanding other's minds have more social deficits and may be less able to make effective use of treatment. [ABSTRACT FROM AUTHOR]

Abstract: There is overwhelming evidence that many local-scale human activities (e.g. fishing) have a deleterious effect on coral reef fish assemblages. Our understanding of how broad social phenomena (e.g. socioeconomic development) affect the diversity and function of coral reef fish assemblages however, is still poor. Here, we use structural equation models to reveal how human population density, socioeconomic development, and market access affect fishing pressure and coral cover to, in turn, explain the diversity and biomass of key functional groups of reef fish assemblages within Solomon Islands. Fishing pressure is predominantly driven by both market access and local population density, and has a clear negative effect on the diversity and function of coral reef fishes. The strong positive effect of market access on fishing pressure makes clear the importance of understanding social-ecological linkages in the context of increasingly connected societies. This study highlights the need to address broad social phenomena rather than focusing on proximate threats such as fishing pressure, to ensure the continued flow of coral reef goods and services in this time of rapid global social and environmental change. [Copyright &y& Elsevier]

Abstract: Elicitation of expert knowledge has proven to be useful in a variety of disciplines including ecology, conservation management and policy. Here we report the development of a protocol and software tool that aids elicitation of expert knowledge of complex systems of count data, focusing on a case study of elicitation of species richness estimates for coral reefs. The software uses newly developed elicitation procedures to elicit probability distributions of counts in a structured and ordered protocol. We present a novel tool that has considerable advantage over more classical “survey” type methods for canvassing expert opinion, including the ability to produce rapid feedback based on fitted statistical distributions (thereby ensuring that expert''s opinions are captured with accuracy) and a means of estimating credible bounds for estimates (essential when few experts are available). It is user friendly, based on open source software (R and tcl/tk), cross platform (Windows and Mac OSX) and the code can be easily modified to tailor the software to a range of applications. [Copyright &y& Elsevier]

Toxicity thresholds for dissolved oil applied in tropical ocean risk assessments are largely based on the sensitivities of temperate and/or freshwater species. To explore the suitability of these thresholds for tropical habitats we experimentally determined toxicity thresholds for eight tropical species for a partially weathered gas condensate, applied the target lipid model (TLM) to predict toxicity of fresh and weathered condensates and compared sensitivities of the tropical species with model predictions. The experimental condensate-specific hazard concentration (HC5) was 167 μg L−1 total aromatic hydrocarbons (TAH), with the TLM-modelled HC5 (78 μg L−1 TAH) being more conservative, supporting TLM-modelled thresholds for tropical application. Putative species-specific critical target lipid body burdens (CTLBBs) indicated that several of the species tested were among the more sensitive species in the TLM database ranging from 5.1 (coral larvae) to 97 (sponge larvae) μmol g−1 octanol and can be applied in modelling risk for tropical marine ecosystems. [Display omitted] • Toxicity thresholds used for oil spill risk assessments primarily based on toxicity data for non-tropical species • Experimental toxicity data for tropical species compared with target lipid model predictions • Experimental and modelled toxicity thresholds for condensate oil agreed within factor of two • Toxicity modelling found suitable for deriving screening toxicity thresholds for tropical oceans • Further toxicity data and modelling for tropical species that include ultraviolet light exposures needed [ABSTRACT FROM AUTHOR]

The reduction in benthic light from natural sediment resuspension events, dredging activities and clouds was quantified over multiple time periods (days to weeks) from a 3-year in-situ field study in the inshore turbid-zone coral communities of the Great Barrier Reef. The results were then used to examine the tolerance levels of three coral species and a sponge to light reduction and associated changes in spectral light quality (in conjunction with elevated sediment concentrations) in a 28-day laboratory-based study. All species survived the exposures but sub-lethal responses involving changes in pigmentation, lipids and lipid ratios were observed. A pocilloporid coral was the most sensitive taxon, with a 28-d EC10 value for bleaching (dissociation of the symbiosis) of 2.7 mol photons m2 d−1. The possibility of such light reduction levels occurring naturally and/or during maintenance dredging activities was then examined using the 3-year in-situ field study as part of a risk assessment. • Inshore corals naturally experience periods of low light impacting their physiology. • Sensitive pocilloporid corals could potentially be used as an indicator of low light effects. • Dredging programs should include measurements of light availability as a key pressure-parameter. [ABSTRACT FROM AUTHOR]

The Great Barrier Reef (GBR) is threatened by climate change and local pressures, including contaminants in nearshore habitats. This study investigated the combined effects of a GBR-relevant contaminant, the herbicide diuron, under current and two future climate scenarios on the coral Acropora millepora. All physiological responses tested (effective quantum yield (ΔF/Fm′), photosynthesis, calcification rate) were negatively affected with increasing concentrations of diuron. Interactive effects between diuron and climate were observed for all responses; however, climate had no significant effect on ΔF/Fm′ or calcification rates. Photosynthesis was negatively affected as the climate scenarios were adjusted from ambient (28.1 °C, p CO 2 = 397 ppm) to RCP8.5 2050 (29.1 °C, p CO 2 = 680 ppm) and 2100 (30.2 °C, p CO 2 = 858 ppm) with EC50 values declining from 19.4 to 10.6 and 2.6 μg L−1 diuron in turn. These results highlight the likelihood that water quality guideline values may need to be adjusted as the climate changes. [ABSTRACT FROM AUTHOR]

The difference between expert level (L3) reader and artificial intelligence (AI) performance for quantifying coronary plaque and plaque components is unknown. This study evaluates the interobserver variability among expert readers for quantifying the volume of coronary plaque and plaque components on coronary computed tomographic angiography (CCTA) using an artificial intelligence enabled quantitative CCTA analysis software as a reference (AI-QCT). This study uses CCTA imaging obtained from 232 patients enrolled in the CLARIFY (CT EvaLuation by ARtificial Intelligence For Atherosclerosis, Stenosis and Vascular MorphologY) study. Readers quantified overall plaque volume and the % breakdown of noncalcified plaque (NCP) and calcified plaque (CP) on a per vessel basis. Readers categorized high risk plaque (HRP) based on the presence of low-attenuation-noncalcified plaque (LA-NCP) and positive remodeling (PR; ≥1.10). All CCTAs were analyzed by an FDA-cleared software service that performs AI-driven plaque characterization and quantification (AI-QCT) for comparison to L3 readers. Reader generated analyses were compared among readers and to AI-QCT generated analyses. When evaluating plaque volume on a per vessel basis, expert readers achieved moderate to high interobserver consistency with an intra-class correlation coefficient of 0.78 for a single reader score and 0.91 for mean scores. There was a moderate trend between readers 1, 2, and 3 and AI with spearman coefficients of 0.70, 0.68 and 0.74, respectively. There was high discordance between readers and AI plaque component analyses. When quantifying %NCP v. %CP, readers 1, 2, and 3 achieved a weighted kappa coefficient of 0.23, 0.34 and 0.24, respectively, compared to AI with a spearman coefficient of 0.38, 0.51, and 0.60, respectively. The intra-class correlation coefficient among readers for plaque composition assessment was 0.68. With respect to HRP, readers 1, 2, and 3 achieved a weighted kappa coefficient of 0.22, 0.26, and 0.17, respectively, and a spearman coefficient of 0.36, 0.35, and 0.44, respectively. Expert readers performed moderately well quantifying total plaque volumes with high consistency. However, there was both significant interobserver variability and high discordance with AI-QCT when quantifying plaque composition. • There is significant interobserver variability among expert readers when quantifying plaque composition • Interobserver variability increases among expert readers when attempting to identify high risk plaque features • There is high discordance between expert readers and AI guided QCT when characterizing plaque components, especially high risk plaque features [ABSTRACT FROM AUTHOR]

Metastatic malignant melanoma with occult primary, presented as breast mass - a case report and literature review B Background: b Malignant melanoma (MM), one of the fastest growing cancers today, is a malignant neoplasm of melanocytes which is a cell found in the basal layer of the epidermis and produces the pigment melanin. While metastases to the breast from extramammary cancers are very rare, MM is one of the malignancies that can metastasize to the breast. [Extracted from the article]

B Background: b Adenoid cystic carcinoma (ACC) is a rare diagnosis affecting mainly the salivary glands, but can also be found in the breast, lung, brain and head & neck with very similar genomic structure irrespective of the disease origin. Breast adenoid cystic carcinoma (BACC) is a rare subtype of epithelial breast malignancy, usually presenting as a painful breast lump in the fifth to sixth decade of life, primarily in females, however, is reported in men also. Unlike other TNBC with basal-like tumour features, BACC it is usually known to have low-grade histological differentiation, rare lymph node and distant metastasis, mild disease course and its prognosis is better than the salivary gland ACC and also than the other phenotypes of breast cancer. [Extracted from the article]

Introduction: The U.S. has a higher adolescent pregnancy rate than other industrialized countries. School-based health centers can improve access to contraceptives among youth, which can prevent unplanned pregnancies. This cross-sectional study examines the characteristics and predictors of contraceptive provision at school-based health centers in 2016-2017 and changes in and barriers to provision between 2001 and 2017.Methods: In 2020-2021, the authors conducted analyses of the National School-Based Health Care Census data collected from 2001 to 2017. The primary outcome of interest was whether adolescent-serving school-based health centers dispense contraceptives, and a secondary outcome of interest was the policies that prohibit school-based health centers from dispensing contraceptives. A multivariate regression analysis examined the associations between contraceptive provision and various covariates, including geographic region, years of operation, and provider team composition.Results: Less than half of adolescent-serving school-based health centers reported providing contraceptives on site. Those that provided contraceptives were more likely located in the Western and Northeastern regions of the U.S., older in terms of years of operation, and staffed by a wide variety of health provider types. Among school-based health centers that experienced policy barriers to providing access to contraceptive methods, most attributed the source to the school or school district where the school-based health center was located.Conclusions: School-based health centers are an evidence-based model for providing contraceptives to adolescents but not enough are providing direct access. Understanding the predictors, characteristics, and barriers influencing the provision of contraceptives at school-based health centers may help to expand the number doing so. [ABSTRACT FROM AUTHOR]

• Closed landfill sites emissions were more enriched in δ13C CH4 than active sites. • The δ13C signature of fugitive methane did not show a seasonal trend. • An active landfill cell closure caused a significant decrease in CH 4 mole fraction. • Closed landfill sites have a higher oxidised proportion than active sites. • Oxidised proportion is highly site specific. Biological oxidation of methane in landfill cover material can be calculated from the carbon isotopic signature (δ13C CH4) of emitted CH 4. Enhanced microbial consumption of methane in the aerobic portion of the landfill cover is indicated by a shift to heavier (less depleted) isotopic values in the residual methane emitted to air. This study was conducted at four landfill sites in southwest England. Measurement of CH 4 using a mobile vehicle mounted instrument at the four sites was coupled with Flexfoil bag sampling of ambient air for high-precision isotope analysis. Gas well collection systems were sampled to estimate landfill oxidised proportion. Closed or active status, seasonal variation, cap stripping and site closure impact on landfill isotopic signature were also assessed. The δ13C CH4 values ranged from −60 to −54‰, with an average value of −57 ± 2‰. Methane emissions from active cells are more depleted in 13C than closed sites. Methane oxidation, estimated from the isotope fractionation, ranged from 2.6 to 38.2%, with mean values of 9.5% for active and 16.2% for closed landfills, indicating that oxidised proportion is highly site specific. [ABSTRACT FROM AUTHOR]

• 10 UK biogas plant methane emission rates were estimated. • Total methane emissions were in range of 0.1 to 58.7 kg hr -1. • The percentage of losses relative to the calculated production rate ranged from 0.02 to 8.1%. • Biogas plants may averagely account for up to 1.9% of total methane emissions in the UK. The rising number of operational biogas plants in the UK brings a new emissions category to consider for methane monitoring, quantification and reduction. Minimising methane losses from biogas plants to the atmosphere is critical not only because of their contribution of methane to global warming but also with respect to the sustainability of renewable energy production. Mobile greenhouse gas surveys were conducted to detect plumes of methane emissions from the biogas plants in southern England that varied in their size, waste feed input materials and biogas utilization. Gaussian plume modelling was used to estimate total emissions of methane from ten biogas plants based on repeat passes through the plumes. Methane emission rates ranged from 0.1 to 58.7 kg CH 4 hr-1, and the percentage of losses relative to the calculated production rate varied between 0.02 and 8.1%. The average emission rate was 15.9 kg CH 4 hr-1, and the average loss was 3.7%. In general, methane emission rates from smaller farm biogas plants were higher than from larger food waste biogas plants. We also suggest that biogas methane emissions may account for between 0.4 and 3.8%, with an average being 1.9% of the total methane emissions in the UK excluding the sewage sludge biogas plants. [ABSTRACT FROM AUTHOR]

We demonstrate that after more than six decades, estimates of global species richness have failed to converge, remain highly uncertain, and in many cases, are logically inconsistent. Convergence in these estimates could be accelerated by adaptive learning methods where the estimation of uncertainty is prioritised and used to guide future research. [Copyright &y& Elsevier]

This study aimed to characterize the carbon isotopic signatures (δ 13C-CH 4) of several methane waste sources, predominantly in the UK, and during field campaigns in the Netherlands and Turkey. CH 4 plumes emitted from waste sources were detected during mobile surveys using a cavity ring-down spectroscopy (CRDS) analyser. Air samples were collected in the plumes for subsequent isotope analysis by gas chromatography isotope ratio mass spectrometry (GC-IRMS) to characterize δ 13C-CH 4. The isotopic signatures were determined through a Keeling plot approach and the bivariate correlated errors and intrinsic scatter (BCES) fitting method. The δ 13C-CH 4 and δ 2H-CH 4 signatures were identified from biogas plants (−54.6 ± 5.6‰, n = 34; −314.4 ± 23‰ n = 3), landfills (−56.8 ± 2.3‰, n = 43; −268.2 ± 2.1‰, n = 2), sewage treatment plants (−51.6 ± 2.2‰, n = 15; −303.9 ± 22‰, n = 6), composting facilities (−54.7 ± 3.9‰, n = 6), a landfill leachate treatment plant (−57.1 ± 1.8‰, n = 2), one water treatment plant (−53.7 ± 0.1‰) and a waste recycling facility (−53.2 ± 0.2‰). The overall signature of 71 waste sources ranged from −64.4 to −44.3‰, with an average of −55.1 ± 4.1‰ (n = 102) for δ 13C, −341 to −267‰, with an average of −300.3 ± 25‰ (n = 11) for δ 2H, which can be distinguished from other source types in the UK such as gas leaks and ruminants. The study also demonstrates that δ 2H-CH 4 signatures, in addition to δ 13C-CH 4 , can aid in better waste source apportionment and increase the granularity of isotope data required to improve regional modelling. • Isotopic 13C signatures from 71 waste sources in UK, NL and TR determined. • Feedstock types have an impact on carbon isotopic signature of generated biogas. • Open and active landfill status has a great impact on carbon isotopic signature. • Sewage treatment is more enriched in 13C than from other waste sources. • Average δ13C isotopic signature of waste sources is −55.1 ± 4.1‰. [ABSTRACT FROM AUTHOR]

We describe real-world estimates of JYNNEOS vaccine effectiveness (VE) against symptomatic mpox in Los Angeles County (LAC). We conducted a retrospective cohort study of men aged ≥18 years residing in LAC who were at risk for mpox and eligible for the JYNNEOS vaccine from 5/19/2022 to 1/1/2023. Case demographics and route of JYNNEOS administration were obtained through vaccine administration data systems. HIV and sexually transmitted infection (STI) status was obtained through disease reporting systems for HIV and STI diagnoses in LAC. To estimate VE, we calculated weekly incidence of confirmed mpox for unvaccinated, partially vaccinated (episode date ≥14 days after first dose), and fully vaccinated (episode date ≥14 days after second dose) cohorts starting on 8/29/2022, when fully vaccinated coverage exceeded 3 %, and ending on 1/1/2023. Overall, 2,171 men had confirmed mpox, and 1,002 (46 %) of those were persons living with diagnosed HIV (PLWDH). 2,019 (93 %) mpox cases were unvaccinated, 114 (5 %) were partially vaccinated and 38 (2 %) were fully vaccinated. VE was 69 % (95 % CI 59–77) for partially vaccinated and 84 % (95 % CI 80–87) for fully vaccinated individuals. Among PLWDH, VE was 72 % (95 % CI 57–82) for fully vaccinated and 28 % (95 % CI −96 to 73) VE for partially vaccinated individuals. Among persons not living with diagnosed HIV, VE was 88 % (95 % CI 86–90) for fully vaccinated and 80 % (95 % CI 76–83) for partially vaccinated individuals. Of 111 individuals hospitalized with mpox, one was partially vaccinated, and the remaining were unvaccinated. Our results align with other published studies that reported that two doses of the JYNNEOS vaccine provided significant protection against symptomatic mpox. [ABSTRACT FROM AUTHOR]

Abstract: Habitat degradation and fishing are major drivers of temporal and spatial changes in fish communities. The independent effects of these drivers are well documented, but the relative importance and interaction between fishing and habitat shifts is poorly understood, particularly in complex systems such as coral reefs. To assess the combined and relative effects of fishing and habitat we examined the composition of fish communities on patch reefs across a gradient of high to low structural complexity in fished and unfished areas of the Ningaloo Marine Park, Western Australia. Biomass and species richness of fish were positively correlated with structural complexity of reefs and negatively related to macroalgal cover. Total abundance of fish was also positively related to structural complexity, however this relationship was stronger on fished reefs than those where fishing is prohibited. The interaction between habitat condition and fishing pressure is primarily due to the high abundance of small bodied planktivorous fish on fished reefs. However, the influence of management zones on the abundance and biomass of predators and target species is small, implying spatial differences in fishing pressure are low and unlikely to be driving this interaction. Our results emphasise the importance of habitat in structuring reef fish communities on coral reefs especially when gradients in fishing pressure are low. The influence of fishing effort on this relationship may however become more important as fishing pressure increases. [Copyright &y& Elsevier]

We present one year of data from the first continuous measurements of carbon dioxide mixing ratio in central London. Measurements were made at an 87m tower site using the new Vaisala CARBOCAP GMP343 instrument, which was found to provide a compact and inexpensive method for mixing ratio monitoring, in an environment where conventional CO2 sensors could not be accommodated. Measurements were compared with a monitoring site outside London, showing that the city''s CO2 “dome” was an order of magnitude smaller than measured at lower levels in other cities. During the night time in the summer, the mixing ratio in central London was found to be significantly lower than at the rural site. This was thought to be explained by the proximity of biogenic sources to the rural sensor, differences between urban and rural mixing heights and/or the interception of a vertical mixing ratio gradient at two different measurement heights. Estimation of the CO2 loading of the air entering the city therefore proved problematic during the summer. As a result, we propose that monitoring of CO2 emission rate using this type of measurement may only be possible in the winter when the influence of these factors is minimal. [Copyright &y& Elsevier]

[Display omitted] Soft robots built with active soft materials have been increasingly attractive. Despite tremendous efforts in soft sensors and actuators, it remains extremely challenging to construct intelligent soft materials that simultaneously actuate and sense their own motions, resembling living organisms' neuromuscular behaviors. This work presents a soft robotic strategy that couples actuation and strain-sensing into a single homogeneous material, composed of an interpenetrating double-network of a nanostructured thermo-responsive hydrogel poly(N-isopropylacrylamide) (PNIPAAm) and a light-absorbing, electrically conductive polymer polypyrrole (PPy). This design grants the material both photo/thermal-responsiveness and piezoresistive-responsiveness, enabling remotely-triggered actuation and local strain-sensing. This self-sensing actuating soft material demonstrated ultra-high stretchability (210%) and large volume shrinkage (70%) rapidly upon irradiation or heating (13%/°C, 6-time faster than conventional PNIPAAm). The significant deswelling of the hydrogel network induces densification of percolation in the PPy network, leading to a drastic conductivity change upon locomotion with a gauge factor of 1.0. The material demonstrated a variety of precise and remotely-driven photo-responsive locomotion such as signal-tracking, bending, weightlifting, object grasping and transporting, while simultaneously monitoring these motions itself via real-time resistance change. The multifunctional sensory actuatable materials may lead to the next-generation soft robots of higher levels of autonomy and complexity with self-diagnostic feedback control. [ABSTRACT FROM AUTHOR]