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The spread of biogenic matrices for agricultural purposes can lead to plastic input into soils, raising a question on possible consequences for the environment. Nonetheless, the current knowledge concerning the presence of plastics in biogenic matrices is very poor. Therefore, the objective of the present study was a quali-quantitative characterization of plastics in different matrices reused in agriculture as manures, digestate, compost and sewage sludges. Plastics were quantified and characterized using a Fourier Transform Infrared Spectroscopy coupled with an optical microscope (μFT-IR) in Attenuated Total Reflectance mode. Our study showed the presence of plastics in all the investigated samples, albeit with differences in the content among the matrices. We measured a lower presence in animal matrices (0.06–0.08 plastics/g wet weight w.w.), while 3.14–5.07 plastics/g w.w. were measured in sewage sludges. Fibres were the prevalent shape and plastic debris were mostly in the micrometric size. The most abundant polymers were polyester (PEST), polypropylene (PP) and polyethylene (PE). The worst case was observed in the compost sample, where 986 plastics/g w.w. were detected. The majority of these plastics were compostable and biodegradable, with only 8% consisting of fragments of PEST and PE. Our results highlighted the need to thoroughly evaluate the contribution of reused matrices in agriculture to the plastic accumulation in the soil system. [Display omitted] • The spread of biogenic matrices can contribute to plastic accumulation in the agroecosystems. • Manures and digestate are less contaminated with plastics than sewage sludges and compost. • Human wastes can introduce 100 times more plastics in soil with respect to animal ones. • Remarkable amount of compostable and biodegradable plastics found in compost. [ABSTRACT FROM AUTHOR]

A growing number of studies have reported the toxic effects of nanoplastics (NPs) on organisms. However, the focus of these studies has almost exclusively been on the use of polystyrene (PS) nanospheres. Herein, we aim to evaluate the sublethal effects on Daphnia magna juveniles of three different NP polymers: PS-NPs with an average size of 200 nm, polyethylene [PE] NPs and polyvinyl chloride [PVC] NPs with a size distribution between 50 and 350 nm and a comparable mean size. For each polymer, five environmentally relevant concentrations were tested (from 2.5 to 250 μg/L) for an exposure time of 48 h. NP effects were assessed at the biochemical level by investigating the amount of reactive oxygen species (ROS) and the activity of the antioxidant enzyme catalase (CAT) and at the behavioral level by evaluating the swimming behavior (distance moved). Our results highlight that exposure to PVC-NPs can have sublethal effects on Daphnia magna at the biochemical and behavioral levels. The potential role of particle size on the measured effects cannot be excluded as PVC and PE showed a wider size range distribution than PS, with particles displaying sizes from 50 to 350 nm. However, we infer that the chemical structure of PVC, which differs from that of PE of the same range size, concurs to explain the observed effects. Consequently, as PS seems not to be the most hazardous polymer, we suggest that the use of data on PS toxicity alone can lead to an underestimation of NP hazards. [Display omitted] • Different nanoplastic polymers exhibit different toxicity. • The physical and chemical properties of nanoplastics influences their toxicity. • Polystyrene is not the most hazardous plastic polymer. • Polyinyl chloride nanoplastics induce sublethal effects to Daphnia magna. [ABSTRACT FROM AUTHOR]

This study aimed to compare the contamination from plastics and non -synthetic particles in the three freshwater bivalve mollusks Unio elongatulus, (native) and Corbicula fluminea and Dreissena polymorpha (invasive), collected in Lake Maggiore, the second greatest Italian lake. Organisms were collected from eight sites located throughout the lake, during three years (2019–2021). The quali-quantitative characterization of particles has been carried out using a Fourier Transform Infrared Microscope System (μFT-IR). Results showed that both plastics and non -synthetic particles released in the water are taken up by bivalves, even though low intake-up to 6 particles/individuals-were measured for all the three species. Microfibers of both synthetic (polyester, polyamide) and natural (cellulose) origin represented the particles mostly ingested by bivalves. A significant decrease of particle loads was observed in 2020 with respect to 2019 and 2021, significantly different for D. polymorpha and U. elongatulus , suggesting a transient reduction of the particle release in the lake in this year. Our findings highlight the need to improve the understanding of the mechanisms of uptake and clearance of these contaminants by filter feeding organisms, and their adverse consequences in realistic environmental conditions. [Display omitted] • Pollution by plastics and non-synthetic particles in freshwater bivalves. • Microfibers represent the anthropogenic particles mostly ingested by bivalves. • Low accumulation of particles for all the three species regardless the sampling site. • Transient reduction of particles accumulation during lock-down for SARS-covid19. [ABSTRACT FROM AUTHOR]

Introduction Despite encouraging phase I and II study results, vaccination of ovarian cancer patients with abagovomab – an anti-idiotypic mAb that mimics the ovarian cancer CA125 protein – failed to demonstrate efficacy in the phase III trial named MIMOSA (NCT00418574). We postulated that in this trial patients with a more robust immune system did respond to abagovomab but went undetected among a larger number of non-responders. We also postulated that assessment of the immune system status ahead of abagovomab administration might predict patients’ propensity to respond to abagovomab. Materials and methods The immune system status was assessed as percentage and absolute count of CD8 + T cells producing IFN-γ after stimulation with Staphylococcal Enterotoxin B (SEB) in 80 patients on abagovomab and 31 patients on placebo from the MIMOSA trial ahead of treatment. Optimal cutoffs of the two variables were calculated by the web application “Cutoff Finder” as the points with most significant (log-rank test) splits based on relapse-free survival (RFS). The Kaplan–Meier curves and log-rank test served to estimate and compare RFS in patients with percentage and absolute count of IFN-γ producing CD8 + T cells around the cutoffs. Results Patients on abagovomab with IFN-γ producing CD8 + T cell percentage above the cutoff had a better RFS (p = 0.042) than those with IFN-γ producing CD8 + T cell percentage below the cutoff. Patients on abagovomab with IFN-γ producing CD8 + T cell absolute count above the cutoff had a better RFS (p = 0.019) than those with IFN-γ producing CD8 + T cell absolute counts below the cutoff. Consistently, the RFS of patients on abagovomab with IFN-γ producing CD8 + T cell percentage and absolute counts values below the respective cutoffs was identical to that of patients on placebo. Neither the percentage nor the absolute count of IFN-γ producing CD8 + T cells correlated with RFS in patients on placebo. Conclusions A robust immune system is essential to obtain a clinical response in OC patients undergoing abagovomab immunotherapy whereas a robust immune system does not confer per se a survival advantage. Further work will clarify whether the results shown here apply only in the present setting or extend to other types of cancer and/or immunotherapeutic agents. [ABSTRACT FROM AUTHOR]

Abstract Understanding the dynamics of a single large water droplet is needed for accurate simulations of the in-flight icing phenomenon. Obtaining information on the ratio of ejected to deposited water and the post-impact droplet distribution should improve the numerical modeling of the bulk of impinging droplets. In this study, a weakly compressible multi-phase Smoothed Particle Hydrodynamics (SPH) method with shifting algorithm and surface tension model is presented to simulate the single droplet dynamics. The validity of the approach has been proved by modeling the classical problems of Rayleigh–Taylor instability, dam break, and droplet formation by comparing against other numerical and experimental data in the literature. Finally, droplet impingement on a liquid film and dry solid surface has been simulated and compared against the experimental data. The effect of impact angle and film thickness on the crown formation is studied to demonstrate the importance of modeling SLD impingement for in-flight icing conditions. [ABSTRACT FROM AUTHOR]

A mesoscale model for droplet dynamics based on a quasi-molecular approach is proposed. It considers the interaction between quasi-molecules within a single liquid droplet, each quasi-molecule representing an agglomeration of a large number of actual water molecules. The goal is to improve the understanding of the dynamics of large droplet collisions over dry or wet surfaces at velocities typical of aeronautical applications. This detailed analysis will eventually be used to refine the macroscopic Eulerian description of the water impingement process by providing numerical correlations for splashing and bouncing phenomena relevant for in-flight icing applications. Based on the Equipartition Theorem, approaches for extracting macroscopic quantities such as temperature and transport coefficients from the quasi-molecular method are discussed. A proper choice of the free parameters of the model that leads to accurate values of the macroscopic properties is also addressed. [ABSTRACT FROM AUTHOR]

β-thalassemia is a major health problem worldwide, and stem cell transplantation (SCT) is the only curative option. Oral Busulfan (Bu) based conditioning is widely used in this setting. Due to the variability of Bu systemic exposure, intravenous (i.v.) Bu has been proposed as a standard of care, with no need for drug monitoring and dose adjustment. Patients with β-thalassemia from countries with limited resources might be at higher risk of erratic Bu metabolism because of liver dysfunction, severe iron overload, and specific ethnic/genetic features. We studied Bu pharmacokinetics in 53 children with advanced β-thalassemia from Middle Eastern countries who underwent a total of 57 matched related donor SCTs. Forty-two percent of the children required dose adjustment because they did not achieve the therapeutic window after the first dose. With a Bu dose-adjustment policy, regimen-related toxicity was limited. At a median follow-up of 564 days, the probabilities of 2-year survival, current thalassemia-free survival, rejection, and treatment-related mortality were 96%, 88%, 21%, and 4%, respectively. Conditioning with i.v. Bu and dose adjustment is feasible and well tolerated, although recurrence of thalassemia remains an unsolved problem in children with advanced disease. [Copyright &y& Elsevier]